Plaque-Forming-Cell Response to H-2 Antigens of Mice

Studies on Surface Antigens of Mammalian Cells

“…By means of this procedure we studied the kinetics of formation of IgM antibodies to murine 0 [26] and H2 alloantigens [31]. This proce dure was also employed for studies of genetic control of antibody re sponse to murine 0-AKR antigen.…”

“…We developed a cytolytic plaque as say in which cells producing antibodies to surface antigens of nucleated cells were enumerated as plaque forming cells (PFC) [26,[30][31][32], The principle of this procedure is very similar to the hemolytic plaque assay of J erne and N ordin [33]. However, in our method, nucleated cells rather than erythrocytes are employed.…”

Studies of Formation of Transplantation Antibodies in Mouse Parabiosis

“…In most, but not all, experiments both partners were of the same sex. The number of cells producing anti bodies against transplantation antigens in the spleens of parabionts was determined using previously described methods (6)(7)(8). The principle of the method is that antibody producing cells incorporated into the agar along with appropriate target cells cause a localized, complement dependent lysis of tar- …”

“…Cytolytic antibodies in sera of experimental animals were detected by means of the "spot test" described in a previous communication (7). Briefly, droplets containing approximately 20 pl of serum at twofold serial dilutions were placed on the agar layer containing appropriate target cells.…”

“…Coelomic parabiosis was performed between mice [8][9][10] weeks old. In most, but not all, experiments both partners were of the same sex.…”