Plaque formation of Quesland V4 lentogenic strain of Newcastle disease virus adapted in chick embryo fibroblast cells

Mehrabanpour, MJ

doi:10.1007/s00580-016-2355-5

Cited by 1 publication

(3 citation statements)

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“…While these studies have been demonstrated that the titer of the DHAV was a relatively low compared with the titer of the propagated DHAV in the GEE cell line. The established GEE cell line was also tested for the NDV infection and we found that the GEE cells can strongly support replication of the NDV as reported previously [ 8 , 10 ]. While our established GEE cell line exhibited a strong susceptibility to NDV with a viral titer of 10 8.75 TCID50/ml after 72 hpi and 10 8.5 ELD 50 /1 ml.…”

Section: Discussionsupporting

confidence: 69%

“…Our finding that the GEE cell line can be supported replication of the GPV was not surprising, as previous data have shown that other cell lines can also be facilitated propagation of the GPV [ 7 ]. However, we were surprised by the ability of the established GEE cell line to assist replication of other avian viruses, such as DHAV and NDV with high infectivity titers compared to other cell lines [ 6 , 8 , 10 , 14 ]. This indicated that the established GEE cell line is very sensitive to avian viral infection.…”

Section: Discussionmentioning

confidence: 99%

“…Establishment and characterization of new cell lines might also provide an alternative tool to study (i) mechanism of viral pathogenesis, and (ii) immunological responses and associated gene expression in the field of host-virus interactions that will be subsequently essential for vaccine development. Development of new fibroblast cell lines that support isolation and propagation of avian viruses, such as goose parvo virus (GPV), duck hepatitis virus (DHV), and Newcastle disease virus (NDV) have already been characterized previously [ 6 – 10 ]. However, fibroblast cells show characteristic morphological changes of senescence after a few passages of the established cell lines.…”

Section: Introductionmentioning

confidence: 99%

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Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses

et al. 2018

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In this study, we report the establishment and characterization of a new epithelial cell line, goose embryonated epithelial cell line (GEE), derived from embryonic goose tissue. The purified GEE cell line can efficiently grow over 65 passages in the M199 medium supplemented with 10% fetal bovine serum at 37°C. Immunofluorescence assay was used to identify purified GEE cells as epithelial cell line by detecting expression of the Keratin-18 and -19. Further characterizations demonstrated that the GEE cell line can be continuously subcultured with (i) a high capacity to replicate for over 65 passages, (ii) a spontaneous epithelial-like morphology, (iii) constant chromosomal features and (iv) without an evidence of converting to tumorigenic cells either in vitro or in vivo study. Moreover, the GEE cell line can be effectively transfected with plasmids expressing reporter genes of different avian viruses, such as VP3, VP1 and F of goose parvo virus (GPV), duck hepatitis virus (DHV), and Newcastle disease virus (NDV), respectively. Finally, the established GEE cell line was evaluated for avian viruses infection susceptibility. Our results showed that the tested GPV, DHAV and NDV were capable to replicate in the new cell line with titers a comparatively higher to the ones detected in the traditional culture system. Accordingly, our established GEE cell line is apparently a suitable in vitro model for transgenic, and infection manipulation studies.

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Section: Discussionsupporting

confidence: 69%