Plants used against cancer – an extension of the work of Jonathan Hartwell

Graham, James G.; Quinn, Matthew; Fabricant, Daniel S.; Farnsworth, Norman R.

doi:10.1016/s0378-8741(00)00341-x

Cited by 380 publications

(247 citation statements)

References 194 publications

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“…1,2) Plant products are widely used in testing because of their low toxicity and great medicinal value. Much research has concentrated on different plant extracts' abilities to induce apoptosis of cancer cells.…”

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confidence: 99%

Apoptosis Induced by Dioscin in Hela Cells.

Cai

Liu

Zhao

et al. 2002

Biological & Pharmaceutical Bulletin

102

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Dioscin, a saponin extracted from the root of Polygonatum Zanlanscianense Pamp, markedly inhibited proliferation of Hela cells. The results indicated that Hela cells underwent apoptosis in dose-and time-dependent manners when treated with Dioscin. Caspase-3, -8 and -9 activities were also detected. The low enzymatic activity of caspase-8 and high activity of caspase-9 showed that the mitochondrial pathway was activated in apoptosis. The reduced expression of the survival protein Bcl-2 also confirmed this result. These studies may be significant in finding a new drug to treat human cervical cancer.

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mentioning

confidence: 99%

Apoptosis Induced by Dioscin in Hela Cells.

Cai

Liu

Zhao

et al. 2002

Biological & Pharmaceutical Bulletin

102

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“…Plants are excellent sources of raw material when searching for new drugs, and they are commonly used in the treatment of cancer (GRAHAM et al, 2000). Moreover, over 60% of currently used anti-cancer agents are derived from natural sources including plants, marine organisms, and micro-organisms (NEWMAN et al, 2003;NEWMAN, 2005).…”

Section: Introductionmentioning

confidence: 99%

Toxicity effect of Auxemma oncocalyx fraction and its active principle oncocalyxone A on in vitro culture of caprine secondary follicles and in vitro oocyte maturation

Leiva-Revilla

Vieira

Campello

et al. 2017

SCA

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The extract of Auxemma oncocalyx (A. oncocalyx) and its main component, oncocalyxone A (onco A), possess anti-tumoral activity that may affect fertility. There is limited literature on the action of these substances regarding caprine folliculogenesis. In this study, we evaluated the effect of A. oncocaly and onco A on the in vitro culture of isolated secondary follicles (Experiment 1) and on the in vitro maturation (IVM) of oocytes from caprine antral follicles grown in vivo (Experiment 2). Isolated secondary follicles were distributed in six groups: the non-cultured control was immediately fixed in 4% paraformaldehyde. The remaining follicles were cultured for 7 days in α-minimal essential medium (α-MEM + ) alone (control) or with dimethyl sulfoxide (DMSO), doxorubicin (DXR), A. oncocalyx, or onco A. After culture, the follicles were evaluated for antrum formation, growth rate, apoptosis (TUNEL), cellular proliferation (PCNA), as well as the expression of BCL2 and BAX. In addition, cumulus oocyte complexes (COCs) were aspirated and allocated into five treatments for IVM: control, cultured only in maturation base medium (TCM 199 + ); or supplemented with DMSO; DXR; A. oncocalyx or onco A. After IVM, oocyte chromatin configuration and viability were assessed. After 7 days of culture, a reduction was noted in the percent of morphologically intact follicles, antrum formation, growth rate, and numbers of PCNApositive granulosa cells (P < 0.05). After culture, the DXR treatment showed a higher percent of TUNELpositive follicles and relative BAX:BCL2 mRNA ratio's (P < 0.05). After IVM of the COCs, DXR, A. oncocalyx, and onco A treatments showed a greater percent (P < 0.05) of abnormal oocytes and a lower percent of viable oocytes as compared with the control group (P < 0.05). However, only DXR and onco A treatments increased the percent of alive oocytes with abnormal chromatin configuration (P < 0.05). There were no differences in the maturation rates among the control group and DXR, A. oncocalyx, and onco A treatments. In conclusion, under our culture conditions, A. oncocalyx and onco A did not exhibit toxic effect on isolated secondary follicles and on maturation rates of COCs recovered from antral follicles. However, these substances negatively affected the oocyte viability. Thus, culture methods including in vitro secondary follicle culture and in vitro oocyte maturation toxicity test are appropriate methods to evaluate the possible effects of drugs on folliculogenesis. ResumoO extrato da Auxemma oncocalyx (A. oncocalyx) e seu componente, Oncocalyxona A (onco A), possui atividade antitumoral, podendo afetar a fertilidade. Entretanto, estudos sobre a ação dessas substâncias em relação à foliculogênese caprina são desconhecidos. O objetivo desse estudo foi avaliar o efeito da A. oncocalyx e onco A no cultivo in vitro de folículos secundários isolados (Experimento 1) e na maturação in vitro (MIV) de oócitos de folículos antrais caprinos crescidos in vivo (Experimento 2). Folículos secundários isolados foram ...

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“…During the late 1960s, Hartwell published a series of articles for a period of over five years in journal Lloydia. This embarked the first series of articles which included information over 3000 plant species which had the potential to act as natural anticancer agents (8)(9)(10). Malaysia is a tropical country that harbours a rich variety of medicinal plants.…”

Section: Introductionmentioning

confidence: 99%