<p><strong>Abstract.</strong> Application of compound-specific isotope analysis (CSIA) in sediment fingerprinting source apportionment studies is becoming more frequent, as it can potentially provide robust land-use based source attribution of suspended sediments in a freshwater system. Isotopic tracers such as &#948;<sup>13</sup>C values of vegetation-derived organic compounds are considered to be suitable for CSIA based fingerprinting method. However, a rigorous evaluation of tracer conservativeness in terms of the stability of isotopic signature during detachment and transport of soil during erosion process is essential for the suitability of the method. With the aim to identify potential fractionation and shifts in tracer signature during early degradation of organic matter in surface soils, we measured concentrations and &#948;<sup>13</sup>C values of long-chain fatty acids and <i>n</i>-alkanes from fresh plant biomass (as vegetation is a direct source of these compounds to the soils), degraded organic horizon (O horizon) as well as mineral soil (A horizon) from various forest types with different humus forms (five sites). The bulk &#948;<sup>13</sup>C values showed continuous <sup>13</sup>C enrichment through the degradation stages from fresh plant material to the O and A horizon, ranging between 3.5 and 5.6&#8201;&#8240;. Compound-specific &#948;<sup>13</sup>C values showed a general <sup>13</sup>C enrichment for both, long-chain fatty acids (up to 5&#8201;&#8240;) as well as <i>n</i>-alkanes (up to 3.9&#8201;&#8240;) from fresh plant biomass to the O horizon overlying the A horizon. However, only slight or no further changes occurred from the O to the A horizon. We also compared compound-specific &#948;<sup>13</sup>C values between two soil particle-size classes (<&#8201;2&#8201;mm and <&#8201;63&#8201;&#181;m) from four sites and found no significant differences of tracer values between them, with even less fractionation for the long-chain <i>n</i>-alkanes within the soil particle fractions, which points to the conclusion that sampling and analysing bulk soil material might be valid for the isotopic tracer applications. We further conclude, that our results support the suitability of studied isotopic tracers as representative source soil signature in CSIA based sediment source attribution, as they demonstrated necessary stability in plant-soil system during organic matter degradation.</p>