Plantlet multiplication from white pine (Pinus strobus L.) embryos in vitro: bud induction and rooting

Abstract

White pine embryos were grown on 4 different media with 6 different benzyladenine (BA) concentrations. Maximum adventitious shoot initiation and growth were obtained on a modified Lepoivre medium with 20/zM BA. Modified Schenk and Hildebrandt, Murashige and Skoog, and Gresshoff and Doy media were also tested. Shoot elongation was achieved on half-strength basal medium lacking growth regulators. Three rooting experiments involving indole-3-butyric acid (IBA), sucrose concentration, shoot orientation, IBA pulse length, and light or dark were carried out. Treatment of shoots in an upright position with 50/xM IBA for eight days followed by culture in a medium with 3% sucrose in the light produced the most rooting (50% at 3 months). Rooted shoots were transplanted to the greenhouse for further growth.

Exogenous Spermidine Promotes Somatic Embryogenesis of Cunninghamia lanceolata by Altering the Endogenous Phytohormone Content

“…The most suitable explants for induction of somatic embryogenesis may differ for each species. Previous studies have shown that in Pinus, somatic embryos can efficiently be induced from immature embryos at the early cotyledon stage, while for Picea mariana, slighty older embryos at the cotyledon stage are more effective [5]. Based on morphological observation and physiological research the embryonic development of conifers is divided into four stages: embryogenesis suspensor mass (ESM), early suspensor proembryos (ESP), late suspensor proembryos (LSP) and cotyledon embryos [6].…”

Plantlet regeneration from mature zygotic embryos and embryonic explants of masson pine (Pinus massoniana Lamb.)

“…For conifer propagation, it is known that the most critical factors are kinds of explants, media components and cytokinin treatment [1,2]. To date, the best results have been obtained using mature zygotic embryos and embryonic explants, and BA alone or in combination with NAA has been shown very effective in promoting bud differentiation in most of the Pinus species [2][3][4][5].…”

Análise do desenvolvimento de gemas adventícias de >i<Pinus Elliottii>/i< Engelm. var. >i<elliottii in vitro>/i<

“…Outros trabalhos como os de David et al (1982), Abdullah et al (1985), Bermudez & Sommer (1987) Bionde & Thorpe (1982) obtiveram estatisticamente melhores resultados com BAP na indução de gemas em cotilédones a 5x 10-5 M (11,265 mgll), mesmo resultado obtido por Schwarz et alo (1988). Comparando-se com o trabalho Chesick et al (1991) e Saravitz et alo (1991), nota-se que estas concentrações estão altas, em relação as usadas neste trabalho, ou seja 0,5 mgll e 1,0 mgll, para a parte superior dos embriões. Tabela 26 -Valores médios para NG de Pinus elliottU varo elliottii, mantidos em duas diferentes concentrações (2,5 e 5,0 mgll de BAP) nas fases de Ind.1 e Ind.2.…”