“Nature education” has become a striking trend in the field of environmental and sustainable development education in mainland China in recent years, which is considered to be a powerful force to realize the 11th goal of the 2030 Agenda for Sustainable Development, namely, “Sustainable Cities and Communities”. Chinese urban residents’ perceptions of “nature education” are fundamental to the success of “nature education” but we still know very little about it. This study aims to gather insights on the awareness, attitudes, and behaviors towards nature and nature education. In this paper, online questionnaires and random sampling methods were used. An online questionnaire survey was conducted among urban residents in eight representative cities in China, namely, Beijing (n = 313), Shanghai (n = 314), Guangzhou (n = 307), Shenzhen (n = 308), Chengdu (n = 206), Xiamen (n = 207), Hangzhou (n = 203), and Wuhan (n = 206). This study implied that Chinese urban residents are willing to accept nature education. There is a strong sense among the Chinese public in cities that living in harmony with nature, improving wellbeing through healthy and sustainable actions, and enjoying and protecting nature are desirable actions. However, respondents were slightly less likely to consider the emotional benefits and the development of social and functional (technical) skills as important elements of participating in nature education. The results also confirm that Chinese urban residents’ perceptions of nature education can be positively predicted by the individual’s relationship with nature and the individual’s experience related to nature education. These are important key messages for nature education organizations to communicate. The results of the study put forward some questions worthy of in-depth consideration for the development of China’s nature education, which can provide a foundation and direction for the further development of nature education in China.