Planteose as a storage carbohydrate required for early stage of germination of Orobanche minor and its metabolism as a possible target for selective control

Wakabayashi, Takatoshi; Joseph, Benesh; Yasumoto, Shuhei; Akashi, Tomoyoshi; Aoki, Takeshi; Harada, Kaoru; Muranaka, Satoru; Bamba, Takeshi; Fukusaki, Eiichiro; Takeuchi, Yoshinori; Yoneyama, Koichi; Muranaka, Toshiya; Sugimoto, Yukihiro; Okazawa, Atsushi

doi:10.1093/jxb/erv116

Cited by 32 publications

(67 citation statements)

References 54 publications

(55 reference statements)

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“…It also showed that removing soluble starch from the growth medium did not affect NJ production. Table 1 7) If S. ficellus cultures could be stored for a long duration without any treatment, such as sulfite adduction, and still exhibit inhibitory activity, costs might be further reduced. This study, therefore, also evaluated NJ stability and inhibitory activity against the seed germination of parasitic weeds.…”

Section: Discussionmentioning

confidence: 99%

“…The inhibition of radicle elongation was also observed upon treatment with an NJ standard. 7) Parasitic weeds cannot survive unless they parasitize host plants immediately after germination; thus, the inhibition of radicle elongation represents a promising method of controlling parasitic weeds. The components in CMB except for NJ would not contribute to these inhibitory activities.…”

Section: Discussionmentioning

confidence: 99%

“…7,16) Seeds of parasitic weeds were surface sterilized with a solution containing 1% (v/v) sodium hypochlorite and 0.1% (v/v) Tween ® 20 for 2 min, rinsed with distilled water, and dried under vacuum. For O. minor, approximately 50 surface-sterilized seeds were conditioned on a filter disk (10 mm, Whatman GF/D; GE Healthcare, Uppsala, Sweden) that was placed on another filter paper (47 mm, Whatman GF/D) in a Petri dish (50 mm) with 1.5 mL distilled water and kept in the dark at 23°C for one week.…”

Section: Bioactivity Assay For Parasitic Weed Germinationmentioning

confidence: 99%

“…7) NJ constitutes an antibiotic that is classified as an azasugar, which primarily inhibits β-glycosidase. [8][9][10][11] NJ was originally discovered in a Streptomyces species culture isolated from soil collected in Hokkaido, Japan.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced production of nojirimycin via Streptomyces ficellus cultivation using marine broth and inhibitory activity of the culture for seeds of parasitic weeds

et al. 2017

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Root parasitic weeds, such as Orobanche spp. and Striga spp., cause serious damage to crops. Recently, it was demonstrated that nojirimycin (NJ) selectively inhibits seed germination in these weeds. In this study, we modified the medium for Streptomyces ficellus to increase its production of NJ and evaluated the culture as an antiparasitic weed agent. We screened alternatives to Pharmamedia ™ , an additive in the original medium, and found that marine broth stimulated NJ production. Moreover, soluble starch-depleted medium could maintain S. ficellus NJ production. The NJ concentration reached 710 mg/L after four-day batch culture in starch-depleted marine broth medium, which was 17-fold higher than that in the Pharmamedia ™ medium. The culture in the marine broth medium inhibited seed germination of Orobanche spp. and Striga spp. as effectively as a standard solution of NJ.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Bioactivity Assay For Parasitic Weed Germinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced production of nojirimycin via Streptomyces ficellus cultivation using marine broth and inhibitory activity of the culture for seeds of parasitic weeds

et al. 2017

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“…At the same time, a downstream product of sucrose, α-D-glucose-1P, was hydrolysed to α-D-glucose-6P under the action of two upregulated genes, namely, PGM2 (TRINITY_DN79669_c0_g2) and PGM (TRINITY_DN72581_c0_g6), in hulled seeds, but the expression of these genes was very low expression in de-hulled seeds; these genes encode phosphoglucomutase and are further involved in the glycolysis pathway. It has been reported that sucrose may be an intermediate of plant metabolism, and the decrease in sucrose decomposition results in low levels of glucose and fructose and inhibits seed germination [32].…”

Section: Genes and Metabolites In Starch And Sucrose Metabolic Pathwamentioning

confidence: 99%

A Combined Analysis of the Transcriptome and Metabolome Revealed the Molecular Mechanism by which GA3 Disrupts Dormancy in Leymus Chinensis Seeds

Bing¹,

Zhang²,

Wang³

et al. 2020

Preprint

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Background: Leymus chinensis is a perennial forage grass that has good palatability, high yield and high feed value, but seed dormancy is a major problem limiting the widespread cultivation of L. chinensis. The aim of this study was to investigate the underlying molecular mechanism and identify candidate genes associated with dormancy disruption by gibberellic acid (GA3) through transcriptomic and metabolomic analysis.Results: The germination test revealed that the optimum concentration of GA3 for disruption of L. chinensis seed dormancy was 200 μg/L. Compared with seeds soaked in sterile water, a total of 4,327 and 11,919 differentially expressed genes (DEGs) and 871 and 650 differentially abundant metabolites were identified in de-hulled and hulled seeds treated with GA3, respectively. Most of the DEGs were associated with starch and sucrose metabolism, protein processing in the endoplasmic reticulum, endocytosis and ribosomes. Furthermore, isoquinoline alkaloid biosynthesis, tyrosine metabolism, starch and sucrose metabolism, arginine and proline metabolism, and amino sugar and nucleotide sugar metabolism were significantly enriched pathways. Integrative analysis of the transcriptomic and metabolomic data revealed that starch and sucrose metabolism is one of the most important pathways that may play a key role in the energy supply for the transition of L. chinensis seeds from a dormant state to germination by suppressing the expression of Cel61a, egID, cel1, tpsA, SPAC2E11.16c and TPP2, along with enhancing the expression of AMY1.1, AMY1.2, AMY1.6 and GLIP5, and finally inhibiting the synthesis of cellobiose, cellodextrin, and trehalose while promoting the hydrolysis of sucrose, starch, cellobiose, cellodextrin, and trehalose to glucose.Conclusions: This study identified several key genes and provided new insights into the molecular mechanism of seed dormancy release by GA3 in L. chinensis. These putative genes will be valuable resources for improving the seed germination rate in future breeding studies.

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Sunflower resistance to the vampire weed broomrape

Labrousse

Delmail

2017

Oilseed Crops

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Planteose as a storage carbohydrate required for early stage of germination of Orobanche minor and its metabolism as a possible target for selective control

Abstract: HighlightPlanteose metabolism was uncovered as a key metabolic pathway for germination of Orobanche minor. Inhibition of planteose metabolism by nojirimycin resulted in selective inhibition of O. minor germination.

Cited by 32 publications

References 54 publications

Enhanced production of nojirimycin <i>via</i> <i>Streptomyces ficellus</i> cultivation using marine broth and inhibitory activity of the culture for seeds of parasitic weeds

Enhanced production of nojirimycin <i>via</i> <i>Streptomyces ficellus</i> cultivation using marine broth and inhibitory activity of the culture for seeds of parasitic weeds

A Combined Analysis of the Transcriptome and Metabolome Revealed the Molecular Mechanism by which GA3 Disrupts Dormancy in Leymus Chinensis Seeds

Sunflower resistance to the vampire weed broomrape

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