Plant volatile ligands for male-biased MmedOBP14 stimulate orientation behavior of the parasitoid wasp Microplitis mediator

Li, Ruijun; Shan, Shuang; Song, Xuan; Khashaveh, Adel; Wang, Shan-Ning; Yin, Zhihui; Lu, Ziyue; Dhiloo, Khalid Hussain; Zhang, Yongjun

doi:10.1016/j.ijbiomac.2022.11.149

Cited by 9 publications

(4 citation statements)

References 82 publications

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“…Plant volatiles can have either attractive or repellent effects on insects, with the effectiveness depending on their concentration. For instance, Li et al [28] reported that Microplitis mediator (Haliday) is strongly attracted to 10 µg/µL β-ionone, but when the concentration reached 100 µg/µL, β-ionone showed repellency to male wasps. Similarly, compared with the control, Chouioia cunea Yang was not attracted to nonadecane at high and low doses, whereas it was more attracted to nonadecane at an intermediate dose [21].…”

Section: Discussionmentioning

confidence: 99%

Deciphering the Chemical Fingerprint of Astragalus membranaceus: Volatile Components Attractive to Bruchophagus huonchili Wasps

Zhang,

Bai,

Kang

et al. 2023

Insects

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Bruchophagus huonchili is a pest that poses a serious threat to the yield and quality of Astragalus membranaceus seeds. In this study, we employed solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) to identify volatile organic compounds (VOCs) in A. membranaceus pods during the pod-filled period. Additionally, we utilized a Y-tube olfactometer to measure the behavioral response of B. huonchili to different individual VOCs and specific VOC-based formulations. The most effective formulations were further evaluated for their effectiveness in attracting wasps in the field. Our findings revealed that A. membranaceus pods emit 25 VOCs, including green leaf volatiles (GLVs) and terpenoid and aromatic compounds. Among these compounds, five were found to be most attractive to B. huonchili at the following concentrations: 10 µg/µL cis-β-ocimene, 500 µg/µL hexyl acetate, 100 µg/µL hexanal, 1 µg/µL decanal, and 10 µg/µL β-caryophyllene, with respective response rates of 67.65%, 67.74%, 65.12%, 67.57%, and 66.67%. In addition, we evaluated 26 mixed VOC formulations, and three of them were effective at attracting B. huonchili. Furthermore, field experiments showed that one of the formulations was significantly more effective than the others, which could be used for monitoring B. huonchili populations.

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Section: Discussionmentioning

confidence: 99%

Deciphering the Chemical Fingerprint of Astragalus membranaceus: Volatile Components Attractive to Bruchophagus huonchili Wasps

Zhang,

Bai,

Kang

et al. 2023

Insects

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“…Among them, PstrOBP12 was cloned into pET‐28a (+) with Nde I and Xho I, PstrOBP16 was cloned into pET‐28a (+) with BamH I and Xho I, PstrOBP13 and PstrOBP19 were cloned into pET‐30a (+) with BamH I and Xho I. Protein was expressed in the competent cells, which were cultured in lysogeny broth at 20°C for 16 h along with induction using 0.5 mM isopropyl β‐d‐1‐thiogalactopyranoside (IPTG), as described previously (Li, Shan, et al, 2022; Wang et al, 2022). After ultrasonic fragmentation and centrifugation, PstrOBPs were obtained as inclusion bodies and subjected to a denaturation and renaturation scheme before purification (Wang et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

Ligand binding properties of three odorant‐binding proteins in striped flea beetle Phyllotreta striolata towards two phthalate esters

Xiao,

Wu,

Lei

et al. 2024

Insect Molecular Biology

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Odorant‐binding proteins (OBPs) initiate insect olfactory perception and mediate specific binding and selection of odorants via uncertain binding mechanisms. We characterized the binding characteristics of four OBPs from the striped flea beetle Phyllotreta striolata (SFB), a major cruciferous crop pest. Tissue expression analysis revealed that the two ABPII OBPs (PstrOBP12 and PstrOBP19) were highly expressed mainly in the antenna, whereas the two minus‐C OBPs (PstrOBP13 and PstrOBP16) showed a broad expression pattern. Competitive binding assays of cruciferous plant volatiles showed that PstrOBP12, PstrOBP16 and PstrOBP19 had very strong binding capacities for only two phthalate esters (Ki < 20 μM), and PstrOBP13 specifically bound to four aromatic volatiles (Ki < 11 μM). Fluorescence quenching assays displayed that two phthalate esters bound to three PstrOBPs via different quenching mechanisms. PstrOBP12/PstrOBP16–diisobutyl phthalate and PstrOBP19–bis(6‐methylheptyl) phthalate followed static quenching, while PstrOBP12/PstrOBP16–bis(6‐methylheptyl) phthalate and PstrOBP19–diisobutyl phthalate followed dynamic quenching. Homology modelling and molecular docking displayed that PstrOBP12–diisobutyl phthalate was driven by H‐bonding and van der Waals interactions, while PstrOBP16–diisobutyl phthalate and PstrOBP19–bis(6‐methylheptyl) phthalate followed hydrophobic interactions. Finally, behavioural activity analysis demonstrated that phthalate esters exhibited different behavioural activities of SFB at different doses, with low doses attracting and high doses repelling. Overall, we thus revealed the different binding properties of the three PstrOBPs to two phthalate esters, which was beneficial in shedding light on the ligand‐binding mechanisms of OBPs.

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“…12 In the parasitoid wasp Microplitis mediator, MmedOBP14 played an important role in the perception of plant volatiles for male wasps to locate habitat, supplement nutrition, and search for partners. 13 Comparative analyses of evolutionarily conserved insect OBPs provide new insights into the functional study of OBPs. Homologous genes SfurOBP11, NlugOBP8, and LstrOBP2 showed a slower evolution rate and are more conserved in phylogenetic analysis of OBPs from Sogatella furcifera, Nilaparvata lugens, and Laodelphax striatellus.…”

Section: Introductionmentioning

confidence: 99%

“…MperOBP3/7/9 from Myzus persicae coordinate the response to the aphid alarm pheromone E‐β‐farnesene 12 . In the parasitoid wasp Microplitis mediator , MmedOBP14 played an important role in the perception of plant volatiles for male wasps to locate habitat, supplement nutrition, and search for partners 13 . Comparative analyses of evolutionarily conserved insect OBPs provide new insights into the functional study of OBPs.…”

Section: Introductionmentioning

confidence: 99%

Identification of odorant‐binding proteins and functional analysis of antenna‐specific BhorOBP28 in Batocera horsfieldi (Hope)

Yi,

Chen,

et al. 2024

Pest Management Science

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BACKGROUNDThe important wood‐boring pest Batocera horsfieldi has evolved a sensitive olfactory system to locate host plants. Odorant‐binding proteins (OBPs) are thought to play key roles in olfactory recognition. Therefore exploring the physiological function of OBPs could facilitate a better understanding of insect chemical communications.RESULTSIn this research, 36 BhorOBPs genes were identified via transcriptome sequencing of adults' antennae from B. horsfieldi, and most BhorOBPs were predominantly expressed in chemosensory body parts. Through fluorescence competitive binding and fluorescence quenching assays, the antenna‐specific BhorOBP28 was investigated and displayed strong binding affinities forming stable complexes with five volatiles, including (+)‐α‐Pinene, (+)‐Limonene, β‐Pinene, (−)‐Limonene, and (+)‐Longifolene, which could also elicit conformation changes when they were interacting with BhorOBP28. Batocera horsfieldi females exhibited a preference for (−)‐Limonene, and a repellent response to (+)‐Longifolene. Feeding dsOBP19 produced by a bacteria‐expressed system with a newly constructed vector could lead to the knockdown of BhorOBP28, and could further impair B. horsfieldi attraction to (−)‐Limonene and repellent activity of (+)‐Longifolene. The analysis of site‐directed mutagenesis revealed that Leu7, Leu72, and Phe121 play a vital role in selectively binding properties of BhorOBP28.CONCLUSIONBy modeling the molecular mechanism of olfactory recognition, these results demonstrate that BhorOBP28 is involved in the chemoreception of B. horsfieldi. The bacterial‐expressed dsRNA delivery system gains new insights into potential population management strategies. Through the olfactory process concluded that discovering novel behavioral regulation and environmentally friendly control options for B. horsfieldi in the future.This article is protected by copyright. All rights reserved.

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Plant volatile ligands for male-biased MmedOBP14 stimulate orientation behavior of the parasitoid wasp Microplitis mediator

Cited by 9 publications

References 82 publications

Deciphering the Chemical Fingerprint of Astragalus membranaceus: Volatile Components Attractive to Bruchophagus huonchili Wasps

Deciphering the Chemical Fingerprint of Astragalus membranaceus: Volatile Components Attractive to Bruchophagus huonchili Wasps

Ligand binding properties of three odorant‐binding proteins in striped flea beetle Phyllotreta striolata towards two phthalate esters

Identification of odorant‐binding proteins and functional analysis of antenna‐specific BhorOBP28 in Batocera horsfieldi (Hope)

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