Plant virus-specific transport function II. A factor controlling virus host range

Taliansky, M. E.; Malyshenko, S. I.; Pshennikova, E. S.; Atabekov, J.G.

doi:10.1016/0042-6822(82)90232-x

Cited by 65 publications

(22 citation statements)

References 11 publications

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“…The identification of residues, such as glycine 227, proline 193, leucine 160 and aspartic acid 162, and regions that are relatively conserved should facilitate examination of the function of the transport protein by site-directed mutagenesis. In vivo complementation studies (Taliansky et al, 1982) suggested that transport proteins are host-specific rather than virus-specific. Host-specific sequences were not apparent in the alignment and some significance scores for proteins of viruses with different hosts (BMV with TMVC and TMVL) were higher than those for proteins of viruses with the same hosts (TSV with TMVC and TMVL).…”

Section: Discussionmentioning

confidence: 99%

Similarities Between Putative Transport Proteins of Plant Viruses

Melcher

1990

Journal of General Virology

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The nucleic acids of many plant viruses encode proteins with one or more of the following properties: an Mr of approximately 30000, localization in the cell wall of the infected plant and a demonstrated role in cell-tocell transport of infection. A progressive alignment strategy, aligning first those sequences known to be similar, and then aligning the resulting groups of sequences, was used to examine further the relatedness of the amino acid sequences of putative transport proteins of caulimoviruses, of proteins similar to the putative transport protein of alfalfa mosaic virus (AIMV) and of those similar to the tobacco mosaic virus (TMV) 30K protein. The strategy first identified regions in which multiple dipeptides of one group were similar to those of another group. The regions of similarity were brought into alignment by the conservative introduction of gaps. The positions of the introduction of gaps were adjusted to optimize similarity.Statistical significances of the resulting alignments, determined both by comparison with shuffled amino acid sequences and with the sequence alignment off-set by 1 to 15 residues in each direction, suggest that the amino acid sequences of the three groups of viruses are distantly related. Nevertheless, significant relationships between members of the caulimoviral group of sequences and members of each of the AIMV-like and TMV-like groups were found. These relationships and the analysis of the number of insertions/deletions between present sequences and a hypothetical common ancestor suggest that the sequences of the caulimoviral proteins are less diverged from the ancestor than either the AIMV-like or TMV-like proteins. The alignment identified common regions of predicted secondary structure and regions of similar hydropathy, regions possibly crucial for proper functioning of the proteins.

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Section: Discussionmentioning

confidence: 99%

Similarities Between Putative Transport Proteins of Plant Viruses

Melcher

1990

Journal of General Virology

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“…The TPs of bromovirus and tobamovirus can be considered as functionally interchangeable to some extent (Hamilton & Nicols, 1977;Taliansky et al, 1982;De Jong & Ahlquist, 1992). However, it should be emphasized that the efficiency of the heterologous TPs in facilitating foreign virus movement was substantially reduced as compared to normal wild-type virus spread.…”

Section: Discussionmentioning

confidence: 99%

Reduction of tobacco mosaic virus accumulation in transgenic plants producing non-functional viral transport proteins

Malyshenko

Кондакова

Nazarova

et al. 1993

Journal of General Virology

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Transgenic plants producing the 30K temperaturesensitive transport protein (TP) of tobacco mosaic virus (TMV) mutant Ni2519 (affecting cell-to-cell transport) were found to: (i) be susceptible to wild-type TMV U1 at 24 °C (a permissive temperature for Ni2519 TP), (ii) acquire a certain level of resistance to TMV U1 accumulation when maintained at 33 °C (a non-permissive temperature for Ni2519 TP) and (iii) lose the resistance to wild-type TMV after their transfer from 33 °C to 24 °C. It is suggested that reversible temperature-dependent conformational changes in Ni2519 TP are responsible for these phenomena and that production of a TP which is only partially functional in transgenic plants confers on these plants a resistance to the virus owing to reduction of the level of cell-to-cell transport. Transgenic tobacco plants producing the 32K TP ofbrome mosaic virus (BMV) acquired resistance to TMV U 1 suggesting that BMV TP is partially functional in tobacco plants.

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“…Protoplasts from these resistant plants supported virus replication [87]. Preinfection of Tm-2 plants with potato virus X (PVX) allowed systemic movement of TMV [115]. One theory to explain the systemic movement in mixed infection of TMV with PVX is that Tm-2 and possibly Tm-22 cells alter expression of TMV movement protein or are unresponsive to it, whereas putative PVX movement protein functions in a normal manner.…”

Section: Resistance To Virus Diseasementioning

confidence: 99%

Molecular basis for virus disease resistance in plants

Mansky

Hill

1993

Archives of Virology

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Classical studies of virus disease resistance in plants have provided the basis for recent molecular studies of resistance. Three common approaches to the study of resistance have been used. In one approach, nucleotide and/or amino acid sequences of virus strains that overcome disease resistance genes in the host are compared with sequences of strains that do not induce disease in these hosts. In the second approach, resistance/susceptibility of protoplasts is compared with the response of intact plants from which they are derived, to develop hypotheses regarding whether resistance acts at the level of the individual cell or by inhibiting cell-to-cell movement. In the third approach, the mechanism of virus cell-to-cell movement has been studied to clarify one of the basic steps in pathogenesis and to determine the mechanism of disease resistance for certain virus-host interactions.

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Plant virus-specific transport function II. A factor controlling virus host range

Cited by 65 publications

References 11 publications

Similarities Between Putative Transport Proteins of Plant Viruses

Similarities Between Putative Transport Proteins of Plant Viruses

Reduction of tobacco mosaic virus accumulation in transgenic plants producing non-functional viral transport proteins

Molecular basis for virus disease resistance in plants

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