Plant Virus Serology

Regenmortel, M.H.V. Van

doi:10.1016/s0065-3527(08)60850-7

Cited by 129 publications

(30 citation statements)

References 200 publications

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“…It was suggested by Aach (1959) that the virion-speci¢c antibodies recognized antigenic determinants that arose by juxtaposition of residues from neighbouring protein subunits. Because the state of aggregation of the viral subunits in£uenced their antigenic properties, a number of terms were introduced to distinguish between the di¡erent antigenic determinants found at the surface of virions and of dissociated subunits (Van Regenmortel 1966). Since antigenic determinants were referred to as epitopes (Jerne 1960), the new epitopes that arise in the polymerized viral subunits as a result of the quaternary structure were called`neotopes'.…”

Section: Intact Tmv Particles and Dissociated Coat Protein Subunits Hmentioning

confidence: 99%

“…Such epitopes, which have been called cryptotopes (Jerne 1960;Van Regenmortel 1966), become accessible to antibodies only after dissociation of the virus particle.…”

Section: Intact Tmv Particles and Dissociated Coat Protein Subunits Hmentioning

confidence: 99%

“…A third label, metatope, was introduced for epitopes found in both dissociated and polymerized forms of the viral protein (Van Regenmortel 1966, 1990. Initially, the existence of antibodies speci¢c for neotopes, cryptotopes and metatopes was established by cross-absorption experiments (Aach 1959;Takahashi & Gold 1960).…”

Section: Intact Tmv Particles and Dissociated Coat Protein Subunits Hmentioning

confidence: 99%

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The antigenicity of tobacco mosaic virus

Regenmortel

1999

Phil. Trans. R. Soc. Lond. B

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The antigenic properties of the tobacco mosaic virus (TMV) have been studied extensively for more than 50 years. Distinct antigenic determinants called neotopes and cryptotopes have been identi¢ed at the surface of intact virions and dissociated coat protein subunits, respectively, indicating that the quaternary structure of the virus in£uences the antigenic properties. A correlation has been found to exist between the location of seven to ten residue-long continuous epitopes in the TMV coat protein and the degree of segmental mobility along the polypeptide chain. Immunoelectron microscopy, using antibodies speci¢c for the bottom surface of the protein subunit, showed that these antibodies reacted with both ends of the stacked-disk aggregates of viral protein. This ¢nding indicates that the stacked disks are bipolar and cannot be converted directly into helical viral rods as has been previously assumed. TMV epitopes have been mapped at the surface of coat protein subunits using biosensor technology. The ability of certain monoclonal antibodies to block the cotranslational disassembly of virions during the infection process was found to be linked to the precise location of their complementary epitopes and not to their binding a¤nity. Such blocking antibodies, which act by sterically preventing the interaction between virions and ribosomes may, when expressed in plants, be useful for controlling virus infection.

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Section: Intact Tmv Particles and Dissociated Coat Protein Subunits Hmentioning

confidence: 99%

“…Such epitopes, which have been called cryptotopes (Jerne 1960;Van Regenmortel 1966), become accessible to antibodies only after dissociation of the virus particle.…”

Section: Intact Tmv Particles and Dissociated Coat Protein Subunits Hmentioning

confidence: 99%

Section: Intact Tmv Particles and Dissociated Coat Protein Subunits Hmentioning

confidence: 99%

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The antigenicity of tobacco mosaic virus

Regenmortel

1999

Phil. Trans. R. Soc. Lond. B

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“…Three of the McAbs reacted only with the virus presented by a first antibody and not with the isolated coat protein [3]. They were considered to be specific for epitopes named neotopes [38,40] which are present only in the quaternary structure of the protein.…”

Section: Introductionmentioning

confidence: 99%

Limitations of different ELISA procedures for localizing epitopes in viral coat protein subunits

Dekker

Porta

Regenmortel

1989

Archives of Virology

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The reactivity of monoclonal antibodies (McAbs) to the coat protein of tobacco mosaic virus (TMVP) with the isolated coat protein, disks, virions and a number of antigenic variants of TMV was tested in eight different ELISA procedures. Although certain McAbs, when used as detecting antibody in the liquid phase, did not react with some of these antigens, they were able to bind to them when used as the capturing antibody on the solid phase. This finding was attributed to the ability of the trapping McAb to induce a complementary conformation in the antigen presented in the liquid phase. In many cases, the reactivity of the McAbs was found to depend on the format of the ELISA. This finding together with the presence of oligomers in viral coat protein preparations made it impossible to map TMVP epitopes on the surface of the viral subunit by means of competitive ELISA.

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“…For these reasons, we thought the monoclonal antibodies recognized determinants internally oriented in the intact particle (i.e. 'cryptotopes' sensu van Regenmortel, 1966). This could explain the cross-reactivity observed in our experiments in contrast to the results of previous attempts to examine serological relationships among these isolates, which used virus-specific capture antibody to sequester the virus from solution (Lister & Rochow, 1979;Rochow & Carmichael, 1979).…”

Section: Discussionmentioning

confidence: 74%

Demonstration of Serological Relationships among Isolates of Barley Yellow Dwarf Virus by Using Polyclonal and Monoclonal Antibodies

Diaco¹,

Lister

Hill

et al. 1986

Journal of General Virology

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SUMMARYHybridomas secreting monoclonal antibodies against three isolates of barley yellow dwarf virus (BYDV) were established. Two monoclonal antibody preparations were generated against the MAV isolate, one against RPV and six against P-PAV. None of the monoclonal antibody preparations reacted with healthy host components. Reactions of monoclonal antibodies, or unlabelled polyclonal antisera, in an indirect enzyme-linked immunosorbent assay (ELISA) indicated that all three virus isolates share a common epitope. BYDV particles dissociated when incubated in carbonatebicarbonate coating buffer at pH 9.6 but could be stabilized by prior dialysis against 2% formaldehyde or 2% glutaraldehyde. In indirect ELISA, unlabelled polyclonal antisera bound to both stabilized and dissociated particles of homologous and heterologous BYDV isolates. However, conjugated polyclonal antisera were incapable of binding to dissociated particles or to stabilized particles of heterologous isolates. Experiments with monoclonal antibodies in a competition ELISA indicated the presence of at least two epitopes on the coat protein of P-PAV.

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Plant Virus Serology

Cited by 129 publications

References 200 publications

The antigenicity of tobacco mosaic virus

The antigenicity of tobacco mosaic virus

Limitations of different ELISA procedures for localizing epitopes in viral coat protein subunits

Demonstration of Serological Relationships among Isolates of Barley Yellow Dwarf Virus by Using Polyclonal and Monoclonal Antibodies

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