Plant virus directed fabrication of nanoscale materials and devices

Culver, James N.; Brown, Amanda; Zang, Faheng; Gnerlich, Markus; Gerasopoulos, Konstantinos; Ghodssi, Reza

doi:10.1016/j.virol.2015.03.008

Cited by 98 publications

(77 citation statements)

References 144 publications

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“…Hence, plant viruses have developed into one of the major classes of biological carrier templates so that all of their features described up to here have been reviewed extensively and repeatedly. For a more complete survey on looming plant VNP applications, the reader is referred to book chapters and articles that list appropriate original references, and specific recent reviews (e.g., Aljabali et al, ; Chu, Brown, Culver, & Ghodssi, ; Culver et al, ; Czapar & Steinmetz, ; Eiben et al, ; Fan et al, ; Koch et al, ; Lam & Steinmetz, ; K. L. Lee et al, ; Lomonossoff, ; Mao, Liu, & Cao, ; Steele et al, ; van Rijn & Schirhagl, ; Wege & Lomonossoff, ; Wen et al, ; Wen & Steinmetz, ). Instructive details on numerous of the underlying preparation and application procedures may be retrieved for example, from Khudyakov and Pumpens (); Lin and Ratna (); Steinmetz and Manchester (); Wege and Lomonossoff ().…”

Section: Introductionmentioning

confidence: 99%

From stars to stripes: RNA‐directed shaping of plant viral protein templates—structural synthetic virology for smart biohybrid nanostructures

Wege

Koch

2019

WIREs Nanomed Nanobiotechnol

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The self‐assembly of viral building blocks bears exciting prospects for fabricating new types of bionanoparticles with multivalent protein shells. These enable a spatially controlled immobilization of functionalities at highest surface densities—an increasing demand worldwide for applications from vaccination to tissue engineering, biocatalysis, and sensing. Certain plant viruses hold particular promise because they are sustainably available, biodegradable, nonpathogenic for mammals, and amenable to in vitro self‐organization of virus‐like particles. This offers great opportunities for their redesign into novel “green” carrier systems by spatial and structural synthetic biology approaches, as worked out here for the robust nanotubular tobacco mosaic virus (TMV) as prime example. Natural TMV of 300 x 18 nm is built from more than 2,100 identical coat proteins (CPs) helically arranged around a 6,395 nucleotides ssRNA. In vitro, TMV‐like particles (TLPs) may self‐assemble also from modified CPs and RNAs if the latter contain an Origin of Assembly structure, which initiates a bidirectional encapsidation. By way of tailored RNA, the process can be reprogrammed to yield uncommon shapes such as branched nanoobjects. The nonsymmetric mechanism also proceeds on 3'‐terminally immobilized RNA and can integrate distinct CP types in blends or serially. Other emerging plant virus‐deduced systems include the usually isometric cowpea chlorotic mottle virus (CCMV) with further strikingly altered structures up to “cherrybombs” with protruding nucleic acids. Cartoon strips and pictorial descriptions of major RNA‐based strategies induct the reader into a rare field of nanoconstruction that can give rise to utile soft‐matter architectures for complex tasks. This article is categorized under: Biology‐Inspired Nanomaterials > Protein and Virus‐Based Structures Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Biology‐Inspired Nanomaterials > Nucleic Acid‐Based Structures

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Section: Introductionmentioning

confidence: 99%

From stars to stripes: RNA‐directed shaping of plant viral protein templates—structural synthetic virology for smart biohybrid nanostructures

Wege

Koch

2019

WIREs Nanomed Nanobiotechnol

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“…The rod‐shaped TMV has a high‐aspect‐ratio geometry (300 nm length by 18 nm diameter) and large protein copy number (≈2130) per particle that makes it an ideal substrate for the integration of tunable high‐surface‐area materials or densely arranged motif displays into functional devices. These properties have driven research into the use of TMV for the development of diverse applications, including anti‐reflective photochemical electrodes, super‐hydrophobic surfaces, improved energy storage, and transducer surfaces for sensing applications …”

Section: Introductionmentioning

confidence: 99%

Tobacco Mosaic Virus as a Versatile Platform for Molecular Assembly and Device Fabrication

Chu

Brown

Culver

et al. 2018

Biotechnology Journal

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Viruses are unique biological agents that infect living host cells through molecular delivery of a genomic cargo. Over the past two decades advancements in genetic engineering and bioconjugation technologies have allowed the unprecedented use of these “unfriendly” biological molecules, as nanoscopic platforms for the advancement of an array of nanotechnology applications. This mini‐review focuses on providing a brief summary of key demonstrations leveraging the versatile characteristics of Tobacco mosaic virus (TMV) for molecular assembly and bio‐device integration. A comprehensive discussion of genetic and chemical modification strategies along with potential limiting factors that impact the assembly of these macromolecules is presented to provide useful insights for adapting TMV as a potentially universal platform toward developing advanced nanomaterials. Additional discussions on biofabrication techniques developed in parallel to enable immobilization, alignment, and patterning of TMV‐based functional particles on solid surfaces will highlight technological innovations that can be widely adapted for creating nanoscopic device components using these engineered biomacromolecules. Further exploitation in the design of molecular specificity and assembly mechanisms and the development of highly controllable and scalable TMV‐device integration strategies will expand the library of nanoscale engineering tools that can be used for the further development of virus‐based nanotechnology platforms.

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“…2). These range from conductive to electronically, plasmonically, and catalytically (Manocchi et al, 2010) active parts (for reviews, see Culver et al, 2015;Fan et al, 2013).…”

Section: Modification Of the Internal Channelmentioning

confidence: 99%

TMV Particles: The Journey From Fundamental Studies to Bionanotechnology Applications

Lomonossoff

Wege

2018

Advances in Virus Research

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Ever since its initial characterization in the 19th century, tobacco mosaic virus (TMV) has played a prominent role in the development of modern virology and molecular biology. In particular, research on the three-dimensional structure of the virus particles and the mechanism by which these assemble from their constituent protein and RNA components has made TMV a paradigm for our current view of the morphogenesis of self-assembling structures, including viral particles. More recently, this knowledge has been applied to the development of novel reagents and structures for applications in biomedicine and bionanotechnology. In this article, we review how fundamental science has led to TMV being at the vanguard of these new technologies.

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Plant virus directed fabrication of nanoscale materials and devices

Cited by 98 publications

References 144 publications

From stars to stripes: RNA‐directed shaping of plant viral protein templates—structural synthetic virology for smart biohybrid nanostructures

From stars to stripes: RNA‐directed shaping of plant viral protein templates—structural synthetic virology for smart biohybrid nanostructures

Tobacco Mosaic Virus as a Versatile Platform for Molecular Assembly and Device Fabrication

TMV Particles: The Journey From Fundamental Studies to Bionanotechnology Applications

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