Plant virology: an RNA treasure trove

Scholthof, Herman B.; Scholthof, Karen-Beth G.

doi:10.1016/j.tplants.2023.06.019

Cited by 1 publication

(1 citation statement)

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“…It has emerged rapidly as a key regulator of gene expression applicable to reverse genetics for plant gene functional studies. Plant scientists discovered gene silencing-related mechanisms while performing plant transformation experiments in which the introduction of a transgene into a desired genome resulted in the silencing of both the transgene and its homologous endogenes [6,7]. As a result of these first observations, plant geneticists and biologists developed this molecular biology approach to address not only gene silencing method questions, but also to explore the complexity of the biological pathways involved, as well as to demonstrate their multilayer relationships with one another.…”

Section: Virus-induced Gene Silencing (Vigs) Systemmentioning

confidence: 99%

Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in Vegetables

Wang,

Cao,

et al. 2023

Horticulturae

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Increased consumption of vegetables has been recommended worldwide as a part of a healthy diet; therefore, determining gene function among breeding materials is crucial for vegetable improvement to meet the sustainable development of new vegetable varieties. However, genetic transformation is time-consuming and laborious, which limits the exploration of gene function for various vegetable crops. Virus-Induced Gene Silencing (VIGS) can perform large-scale and rapid gene silencing in plants due to a reduction in the experimental period and its independence from the stable genetic transformation, providing an excellent opportunity for functional research. VIGS can accelerate model plant research and make it easier to analyze gene function and validation in vegetable crops. Moreover, with the advent of technologies such as virus-mediated heterologous protein expression and the development of CRISPR/Cas9 technology, virus-mediated genetic tools have ushered in a new era in genetics and crop improvement. This study summarizes recent achievements in VIGS and Virus-Induced Gene Editing (VIGE) in vegetables. We also identify several challenges in the current state of VIGS technology in vegetables, serving as a guide for future research.

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Section: Virus-induced Gene Silencing (Vigs) Systemmentioning

confidence: 99%