Plant Transformation: Needs and Futurity of the Transgenes

Darbani, Behrooz; Farajnia, Safar; Noeparvar, Shahin; Stewart, C. Neal; Mohammadi, Seyed Abolghasem; Zakerbostanabad, Saeed

doi:10.3923/biotech.2008.403.412

Cited by 7 publications

(1 citation statement)

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“…Genetically modified plants are rapidly becoming a common feature of modern agriculture in many parts of the world. In 1996, 1.7 million hectares (M ha) of genetically modified crops were grown worldwide, and the area increased to 81.0 M ha in 2004 (Chapman et al, 2006), 90.0 M ha in 2005 (Darbani et al, 2008a), and 181.5 M ha in 2014 (James, 2014). The most commonly used methods for genetically modified plants are the biolistics method (particle bombardment) and Agrobacteriummediated transformation (Shou et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation of five inbred maize lines with the Brittle 2 gene

Nguyen¹,

Tran²,

Nguyen³

2016

Turk J Biol

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The Brittle 2 (Bt2) gene encodes a small subunit of ADP-glucose pyrophosphorylase (AGPase) enzyme, one of the enzymes that play an important role in endosperm starch biosynthesis. We carried out a study to transfer the Bt2 gene into several inbred maize lines and regenerate transgenic maize plants possessing the transgene, which can be used to further study the role of the Bt2 gene in starch biosynthesis. Agrobacterium tumefaciens strains C58 and EHA105 harboring the transformation vector pCambia2300/Ubi-Bt2-Nos were used to transfer the Bt2 gene into five inbred maize lines, H14, H26, H240, H95, and N18. Transformed calli were selected on selection medium containing 100 mg/L of kanamycin. The stable integration of the Bt2 gene into transgenic plants was confirmed by Southern hybridizations. We recorded the different transformation frequencies in the experiments with A. tumefaciens strains C58 and EHA105. Transgenic plants were regenerated from transformed calli of all five studied maize lines. Transformation frequencies varied from 1.09% to 2.33%, depending on maize genotypes. The results obtained show that our transformation system is effective for numerous maize genotypes. H95 and H240 are good lines for gene transfer in maize.

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Section: Introductionmentioning

confidence: 99%