Plant Tissue Culture and DNA Delivery Methods

Soman, Jayanthi; Jagadeesan, Hema; Subramanian, Selvi

doi:10.1007/978-981-13-9624-3_1

Cited by 3 publications

(1 citation statement)

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“…The complete process comprising the introduction, integration, and expression of foreign gene(s) in the host is termed genetic transformation or transgenesis [ 137 ]. Together, the development and application of molecular genetic engineering, gene transfer methods, and in vitro tissue culture techniques have allowed for the efficient genetic transformation of a broad diversity of crop plants [ 138 , 139 ].…”

Section: Molecular Toolsmentioning

confidence: 99%

Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops

Villalobos-López

Arroyo-Becerra

Quintero-Jiménez

et al. 2022

IJMS

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The major challenges that agriculture is facing in the twenty-first century are increasing droughts, water scarcity, flooding, poorer soils, and extreme temperatures due to climate change. However, most crops are not tolerant to extreme climatic environments. The aim in the near future, in a world with hunger and an increasing population, is to breed and/or engineer crops to tolerate abiotic stress with a higher yield. Some crop varieties display a certain degree of tolerance, which has been exploited by plant breeders to develop varieties that thrive under stress conditions. Moreover, a long list of genes involved in abiotic stress tolerance have been identified and characterized by molecular techniques and overexpressed individually in plant transformation experiments. Nevertheless, stress tolerance phenotypes are polygenetic traits, which current genomic tools are dissecting to exploit their use by accelerating genetic introgression using molecular markers or site-directed mutagenesis such as CRISPR-Cas9. In this review, we describe plant mechanisms to sense and tolerate adverse climate conditions and examine and discuss classic and new molecular tools to select and improve abiotic stress tolerance in major crops.

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Section: Molecular Toolsmentioning

confidence: 99%