Plant Tissue Culture

Bhatia, Saurabh

doi:10.1016/b978-0-12-802221-4.00002-9

Cited by 36 publications

(24 citation statements)

References 212 publications

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“…Both PGRs were also used at different concentrations for callus induction from Ephedra foliata (Lodha et al, 2014). Callus induction is necessary as the first step for the initiation of suspension cultures for the production of active constituents in tissue culture experiments (Bhatia, 2015). It has been shown that the induction of secondary metabolite production is more effective in the stationary growth phase.…”

Section: Resultsmentioning

confidence: 99%

Ephedrine production from suspension cultures of Ephedra alata L. callus

Hegazi¹,

Ghareb²,

Gabr³

2020

bta

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Ephedra alata is an important Egyptian medicinal plant with enormous applications in pharmaceutical industry. Shake-flask suspension cultures of E. alata were established from internodal segment-derived callus. The suspension cultures were treated with two biotic elicitors, namely Aspergillus niger and yeast extract, to enhance the synthesis of ephedrine -the bioactive constituent of E. alata. Ephedrine was quantified using the high performance liquid chromatography technique. Ephedrine concentration was not directly related to biomass production. Aspergillus niger and yeast extract enhanced the production of ephedrine by 2.5-to 7-fold after different durations of incubation as compared to ephedrine content in the mother plant. Treatment with the fungal elicitor A. niger extract induced the maximum accumulation of ephedrine at the concentration of 1.67%; it also increased ephedrine biosynthesis by 7-fold in 24-day-old culture as compared to the ephedrine content in the mother plant and caused higher ephedrine biosynthesis than that in the control treatment without elicitors. The present protocol could be applicable for the large-scale production of ephedrine from E. alata.

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Section: Resultsmentioning

confidence: 99%

Ephedrine production from suspension cultures of Ephedra alata L. callus

Hegazi¹,

Ghareb²,

Gabr³

2020

bta

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“…The exact composition of complex organics, such as casein hydrolysate, casamino acids, coconut water, and yeast extract, is typically undefined and varies depending on the manufacturer/supplier and potentially the batch. However, the amino acids, hormones, vitamins, fatty acids, carbohydrates, and other growth supplements they provide may enhance growth and regeneration of plants ( Bhatia, 2015 ). The addition of casein hydrolysate was initially shown to give a more consistent high rate of microcallus formation from tobacco ( Nicotiana tabacum ) protoplasts ( Galun and Raveh, 1975 ), and is currently an addition to protoplast culture media regularly ( Table 2 ).…”

Section: Protoplast Culturementioning

confidence: 99%

Protoplast Regeneration and Its Use in New Plant Breeding Technologies

Reed

Bargmann

2021

Front. Genome Ed.

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The development of gene-editing technology holds tremendous potential for accelerating crop trait improvement to help us address the need to feed a growing global population. However, the delivery and access of gene-editing tools to the host genome and subsequent recovery of successfully edited plants form significant bottlenecks in the application of new plant breeding technologies. Moreover, the methods most suited to achieve a desired outcome vary substantially, depending on species' genotype and the targeted genetic changes. Hence, it is of importance to develop and improve multiple strategies for delivery and regeneration in order to be able to approach each application from various angles. The use of transient transformation and regeneration of plant protoplasts is one such strategy that carries unique advantages and challenges. Here, we will discuss the use of protoplast regeneration in the application of new plant breeding technologies and review pertinent literature on successful protoplast regeneration.

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“…The industrial process of therapeutic compounds production from callus cells revolves around the choice of the right bioreactor for the fermentation process, optimal temperature and pH as well as providing the right amounts of nutrients to suit the cultured plant [13]. Normally, callus cultures are converted into suspension culture systems, which employ either batch or continuous fermentation processes that yield.…”

Section: Callus Culture As Means Of Therapeutic Compounds Productionmentioning

confidence: 99%

Callus Culture for the Production of Therapeutic Compounds

Benjamin¹,

Ishaku²,

Peingurta³

et al. 2019

AJPB

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Plant-derived compounds retain a special place in the treatment of various diseases across the world. Their application cuts across every class of disease, where they are found to be often equal or of greater potency, safer and cheaper than so-called "orthodox" medicines. These advantages have led to great interest in the use of callus culture as a biotechnological tool for the harnessing of these useful therapeutic compounds. Callus culture techniques aim to increase the yield of active constituents in cultured plant cells and to produce novel products on a large scale. These techniques have been applied to produce various classes of therapeutic compounds from diverse plant species through empirical determination of ideal culture conditions and other methods. This review presents at a glance the recent advances being made in the field of callus culture for the production of therapeutic compounds, with the aim of showing that it is time for the full potentials of callus culture to be exploited on a scale that will prove a useful weapon in the arsenal of clinical therapeutics.

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Plant Tissue Culture

Cited by 36 publications

References 212 publications

Ephedrine production from suspension cultures of Ephedra alata L. callus

Ephedrine production from suspension cultures of Ephedra alata L. callus

Protoplast Regeneration and Its Use in New Plant Breeding Technologies

Callus Culture for the Production of Therapeutic Compounds

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