Plant Tissue Culture

Loyola‐Vargas, Víctor M.; De‐la‐Peña, Clelia; Galáz-Ávalos, Rosa M.; Quiroz-Figueroa, Francisco Roberto

doi:10.1007/978-1-60327-375-6_50

Cited by 27 publications

(13 citation statements)

References 212 publications

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“…Numerous factors that affect SE have been investigated in order to understand the basis of this process and manipulate it to develop and establish efficient plant regeneration protocols as a fundamental step for its biotechnological application (Loyola-Vargas et al 2008). A differentiated and specialized somatic plant cell or a group of somatic cells with specific functions must receive a stimulus from a set of plant growth regulators (PGRs), mainly auxins, perceive it, and then initiate the transduction to the nucleus where the specific regulatory and structural genes will be transcribed and subsequently will be translated into proteins involved in the differentiation that ultimately will lead to the formation of a new somatic embryo.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Somatic Embryogenesis. An Overview

Loyola‐Vargas

Ochoa-Alejo

2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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Section: Somatic Embryogenesismentioning

confidence: 99%

Somatic Embryogenesis. An Overview

Loyola‐Vargas

Ochoa-Alejo

2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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“…This process is known as somatic embryogenesis (SE), and is a powerful biotechnological process that allows the mass production of economically important cultivars. Furthermore, SE is also an attractive system to study the morphology, biochemistry, genetic and molecular mechanisms of embryo development [ 2 ]. This system is built on the concept of cellular totipotency, which is the ability of a single cell to divide and produce a complete and functional plant.…”

Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis: Identified Factors that Lead to Embryogenic Repression. A Case of Species of the Same Genus

et al. 2015

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Somatic embryogenesis is a powerful biotechnological tool for the mass production of economically important cultivars. Due to the cellular totipotency of plants, somatic cells under appropriate conditions are able to develop a complete functional embryo. During the induction of somatic embryogenesis, there are different factors involved in the success or failure of the somatic embryogenesis response. Among these factors, the origin of the explant, the culture medium and the in vitro environmental conditions have been the most studied. However, the secretion of molecules into the media has not been fully addressed. We found that the somatic embryogenesis of Coffea canephora, a highly direct embryogenic species, is disrupted by the metabolites secreted from C. arabica, a poorly direct embryogenic species. These metabolites also affect DNA methylation. Our results show that the abundance of two major phenolic compounds, caffeine and chlorogenic acid, are responsible for inhibiting somatic embryogenesis in C. canephora.

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“…This method is characterized by fast reproduction speed and high reproduction coe cient, which can maintain the superiority of the variety [11,12]. However, complicated and time-consuming operating procedures, as well as strict and meticulous technical requirements hinder the wide application of tissue culture [13]. For example, tissue culture requires a well-equipped laboratory, which requires high capital and operating costs [14].…”

Section: Introductionmentioning

confidence: 99%

Acacia Mangium × A. Auriculiformis Micropropagation in a Non-Sterile Environment

LieJian

Hong

et al. 2021

Preprint

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Background: Autoclaving is used to eliminate contamination during tissue culturing, however, it is a complicated process, time-consuming and costly. Chemical sterilization of tissue culture can effectively eliminate contamination, is a simple procedure, and cost effective. However, studies on the chemical sterilization mostly focus on bud induction, while the effects of chemical sterilization overall process of tissue culture, including bud induction, proliferation, and rooting, remain to be determined. Here, we investigate the effect of chemical sterilization on bud induction, proliferation, and rooting of Acacia mangium × A. auriculiformis.Results: The results showed that chlorothalonil (0.2 g/L) was a suitable chemical sterilant, and bud induction medium was 1/8 Murashige and Skoog medium + agar 7 g/L + chlorothalonil 0.2 g/L + 6-benzylaminopurine 0.5 mg/L The highest induction rate (99.54%) was observed in the third to fifth buds’ stem segments collected in October treated with 0.8 g/L carbendazim for 3 min, with a contamination rate of 0. The rooting medium was agar 7 g/L + chlorothalonil 0.2 g/L+ indolebutyric acid 1.5 mg/L + naphthylacetic acid 0.5 mg/L, and the rooting rate was 97.62%. The proliferation rate and subculture duration showed a positive correlation, while the proliferation rate was 3.58 times higher at the fourth subculture rooting. Conclusions: Our results suggest that chlorothalonil can effectively replace autoclaving during bud induction, proliferation, and rooting of A. mangium × A. auriculiformis. The findings of this study provide technical support for rapid seedlings propagation, accelerates the breeding process of Acacia, and can be applied in other tree species.

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Plant Tissue Culture

Cited by 27 publications

References 212 publications

Somatic Embryogenesis. An Overview

Somatic Embryogenesis. An Overview

Somatic Embryogenesis: Identified Factors that Lead to Embryogenic Repression. A Case of Species of the Same Genus

Acacia Mangium × A. Auriculiformis Micropropagation in a Non-Sterile Environment

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