Plant synthetic biology for molecular engineering of signalling and development

Nemhauser, Jennifer L.; Torii, Keiko U.

doi:10.1038/nplants.2016.10

Cited by 55 publications

(50 citation statements)

References 96 publications

(98 reference statements)

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“…Nemhauser and Torii, 2016;Yang et al, 2016). The dehydration-sensing mechanisms involved in driving the accumulation of the hormone abscisic acid (ABA; see symbols in Table I) are of special importance as it is implicated in stomatal closure during drought (Rodriguez-Dominguez et al, 2016) or increasing vapor pressure deficit , and may contribute to the decline of leaf hydraulic conductance (Shatil-Cohen et al, 2011;Pantin et al, 2013).…”

mentioning

confidence: 99%

ABA Accumulation in Dehydrating Leaves Is Associated with Decline in Cell Volume, Not Turgor Pressure

2017

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confidence: 99%

ABA Accumulation in Dehydrating Leaves Is Associated with Decline in Cell Volume, Not Turgor Pressure

2017

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“…Synthetic biology is a field that aims to bring formal engineering approaches to the design, construction and analysis of biological systems 1 . Most recent advances in synthetic biology have used microbes as a platform.…”

Section: Introductionmentioning

confidence: 99%

Systematic tools for reprogramming plant gene expression in a simple model,Marchantia polymorpha

Sauret-Güeto

Frangedakis

Silvestri

et al. 2020

Preprint

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We present the OpenPlant toolkit, a set of interlinked resources and techniques to develop Marchantia as testbed for bioengineering in plants. Marchantia is a liverwort, a simple plant with an open form of development that allows direct visualization of gene expression and dynamics of cellular growth in living tissues. We describe new techniques for simple and efficient axenic propagation and maintenance of Marchantia lines with no requirement for glasshouse facilities. Marchantia plants spontaneously produce clonal propagules within a few weeks of regeneration, and lines can be amplified million-fold in a single generation by induction of the sexual phase of growth, crossing and harvesting of progeny spores. The plant has a simple morphology and genome with reduced gene redundancy, and the dominant phase of its life cycle is haploid, making genetic analysis easier. We have built robust Loop assembly vector systems for nuclear and chloroplast transformation and genome editing. These have provided the basis for building and testing a modular library of standardized DNA elements with highly desirable properties. We have screened transcriptomic data to identify a range of candidate genes, extracted putative promoter sequences, and tested them in vivo to identify new constitutive promoter elements. The resources have been combined into a toolkit for plant bioengineering that is accessible for laboratories without access to traditional facilities for plant biology research. The toolkit is being made available under the terms of the OpenMTA and will facilitate the establishment of common standards and the use of this simple plant as testbed for synthetic biology.

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“…Although such methods are emerging in plant systems, including fluorescently tagged hormones and metabolites to visualize signaling events (Nemhauser and Torii, 2016), very few small molecule-based orthogonal systems have been described. However, GA and ABA have been used as small molecules in Figure 3.…”

Section: Target-based Screens and Synthetic Biologymentioning

confidence: 99%