Plant Survival in a Changing Environment: The Role of Nitric Oxide in Plant Responses to Abiotic Stress

Simontacchi, Marcela; Galatro, Andrea; Ramos-Artuso, Facundo; Santa-Marı́a, Guillermo E.

doi:10.3389/fpls.2015.00977

Cited by 222 publications

(120 citation statements)

References 222 publications

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“…In addition, NO in mammals can be produced from nitrite nonenzymatically under acidic conditions (Zweier, Li, Samouilov & Liu, ). In higher plants, the production of NO via an enzymatic l ‐arginine‐dependent synthesis has been also detected, but homologs to known NO synthases have not been identified yet in screens of fully sequenced plant genomes (Simontacchi, Galatro, Ramos‐Artuso & Santa‐María, ). In fact, the major route of NO production in plants is via the reduction of nitrite by nitrate reductase (NR) (Lamotte et al, ; Rockel, Strube, Rockel, Wildt & Kaiser, ; Yamasaki, ; Yamasaki & Sakihama, ).…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide as a developmental and metabolic signal in filamentous fungi

Zhao

Lim

et al. 2020

Molecular Microbiology

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The short‐lived hydrophobic gas nitric oxide (NO) is a broadly conserved signaling molecule in all domains of life, including the ubiquitous and versatile filamentous fungi (molds). Several studies have suggested that NO plays a vast and diverse signaling role in molds. In this review, we summarize NO‐mediated signaling and the biosynthesis and degradation of NO in molds, and highlight the recent advances in understanding the NO‐mediated regulation of morphological and physiological processes throughout the fungal life cycle. In particular, we describe the role of NO in molds as a signaling molecule that modulates asexual and sexual development, the formation of infection body appressorium, and the production of secondary metabolites (SMs). In addition, we also summarize NO detoxification and protective mechanisms against nitrooxidative stress.

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Section: Introductionmentioning

confidence: 99%

Nitric oxide as a developmental and metabolic signal in filamentous fungi

Zhao

Lim

et al. 2020

Molecular Microbiology

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“…Nitric oxide (NO) as a signaling molecule plays a crucial role in these responses acting alone or together with reactive oxygen species (ROS) to regulate hormonal signaling pathways, gene expression changes or protein activities. The regulatory role of NO has been demonstrated in response to abiotic and biotic stresses as well as in plant developmentally processes throughout the entire plant life (Corpas et al, 2011; Yu et al, 2014; Simontacchi et al, 2015). NO can influence protein activity, translocation and protein function by posttranslational modifications.…”

Section: Introductionmentioning

confidence: 99%

ROS-Mediated Inhibition of S-nitrosoglutathione Reductase Contributes to the Activation of Anti-oxidative Mechanisms

et al. 2016

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Nitric oxide (NO) has emerged as a signaling molecule in plants being involved in diverse physiological processes like germination, root growth, stomata closing and response to biotic and abiotic stress. S-nitrosoglutathione (GSNO) as a biological NO donor has a very important function in NO signaling since it can transfer its NO moiety to other proteins (trans-nitrosylation). Such trans-nitrosylation reactions are equilibrium reactions and depend on GSNO level. The breakdown of GSNO and thus the level of S-nitrosylated proteins are regulated by GSNO-reductase (GSNOR). In this way, this enzyme controls S-nitrosothiol levels and regulates NO signaling. Here we report that Arabidopsis thaliana GSNOR activity is reversibly inhibited by H2O2 in vitro and by paraquat-induced oxidative stress in vivo. Light scattering analyses of reduced and oxidized recombinant GSNOR demonstrated that GSNOR proteins form dimers under both reducing and oxidizing conditions. Moreover, mass spectrometric analyses revealed that H2O2-treatment increased the amount of oxidative modifications on Zn2+-coordinating Cys47 and Cys177. Inhibition of GSNOR results in enhanced levels of S-nitrosothiols followed by accumulation of glutathione. Moreover, transcript levels of redox-regulated genes and activities of glutathione-dependent enzymes are increased in gsnor-ko plants, which may contribute to the enhanced resistance against oxidative stress. In sum, our results demonstrate that reactive oxygen species (ROS)-dependent inhibition of GSNOR is playing an important role in activation of anti-oxidative mechanisms to damping oxidative damage and imply a direct crosstalk between ROS- and NO-signaling.

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“…In animals, NO is synthesized by the enzyme nitric oxide synthase (NOS). However, the existence of a plant NOS enzymes encounters debate, because analysis of fully sequenced plant genome suggests no homology with known NOSs (Correa-Aragunde et al 2013;Foresi et al 2010;Simontacchi et al 2015). Thereby, the reports of the source of endogenous NO under Cu stress are conflicting.…”

Section: Introductionmentioning

confidence: 91%

Early generation of nitric oxide contributes to copper tolerance through reducing oxidative stress and cell death in hulless barley roots

2016

J Plant Res

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The objective of this study was to investigate the specific role of nitric oxide (NO) in the early response of hulless barley roots to copper (Cu) stress. We used the fluorescent probe diaminofluorescein-FM diacetate to establish NO localization, and hydrogen peroxide (H2O2)-special labeling and histochemical procedures for the detection of reactive oxygen species (ROS) in the root apex. An early production of NO was observed in Cu-treated root tips of hulless barley, but the detection of NO levels was decreased by supplementation with a NO scavenger, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO). Application of sodium nitroprusside (a NO donor) relieved Cu-induced root inhibition, ROS accumulation and oxidative damage, while c-PTIO treatment had a synergistic effect with Cu and further enhanced ROS levels and oxidative stress. In addition, the Cu-dependent increase in activities of superoxide dismutase, peroxidase and ascorbate peroxidase were further enhanced by exogenous NO, but application of c-PTIO decreased the activities of catalase and ascorbate peroxidase in Cu-treated roots. Subsequently, cell death was observed in root tips and was identified as a type of programed cell death (PCD) by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The addition of NO prevented the increase of cell death in root tips, whereas inhibiting NO accumulation further increased the number of cells undergoing PCD. These results revealed that NO production is an early response of hulless barley roots to Cu stress and that NO contributes to Cu tolerance in hulless barley possibly by modulating antioxidant defense, subsequently reducing oxidative stress and PCD in root tips.

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Plant Survival in a Changing Environment: The Role of Nitric Oxide in Plant Responses to Abiotic Stress

Cited by 222 publications

References 222 publications

Nitric oxide as a developmental and metabolic signal in filamentous fungi

Nitric oxide as a developmental and metabolic signal in filamentous fungi

ROS-Mediated Inhibition of S-nitrosoglutathione Reductase Contributes to the Activation of Anti-oxidative Mechanisms

Early generation of nitric oxide contributes to copper tolerance through reducing oxidative stress and cell death in hulless barley roots

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