Plant sterols: a neutron diffraction study of sitosterol and stigmasterol in soybean phosphatidylcholine membranes

Marsan, M.P.; Bellet-Amalric, E.; Muller, Isabelle S.; Zaccaı̈, Giuseppe; Milon, Alain

doi:10.1016/s0301-4622(98)00188-4

Cited by 21 publications

(14 citation statements)

References 38 publications

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“…We have seen in this work that sitosterol is more effective in ordering a fluid DPPC membrane than stigmasterol. It might be surprising that stigmasterol with a more extended acyl chain than sitosterol would be less efficient in ordering the membrane, however a similar behavior have also been reported for these sterols [14,69]. From our results the possibility that the bend in the sitosterol molecule could fulfill gaps in the more fluid part of the DPPC molecule (from C12 to C16 atoms) facilitating van der Waals interactions and restricting the molecule movement, that would increase the order of the membrane in a liquid ordered phase could be suggested.…”

Section: Discussionsupporting

confidence: 55%

Molecular aspects of the interaction between plants sterols and DPPC bilayers

Aranda

Ortíz

Martı́nez

et al. 2011

Journal of Colloid and Interface Science

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Section: Discussionsupporting

confidence: 55%

Molecular aspects of the interaction between plants sterols and DPPC bilayers

Aranda

Ortíz

Martı́nez

et al. 2011

Journal of Colloid and Interface Science

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“…Stigmasterol is far less efficient than sitosterol in increasing membrane order and has no effect on membrane permeability (Schuler et al 1991). Such a differential behavior is also attested by the two-fold lower ability of stigmasterol to increase the width of the membrane bilayer compared to sitosterol (0.5 A° versus 1 A°) (Marsan et al 1998). However, cholesterol and ∆ 22 -dehydrocholesterol exhibit similar efficiencies, indicating that this differential behavior is strictly dependent on the presence of an alkyl group in the side chain (Krajewski-Bertrand et al 1992).…”

Section: Structural Roles As Membrane Componentsmentioning

confidence: 98%

5 Sterol metabolism and functions in higher plants

Hartmann

2003

Lipid Metabolism and Membrane Biogenesis

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Higher plants synthesize a bewildering array of sterols, with sitosterol, stigmasterol, and 24-methylcholesterol as major compounds. All plant tissues contain free and conjugated sterols. The first steps of sterol biosynthesis occur via the classical acetate/mevalonate pathway, concomitantly with synthesis of many other isoprenoids. A crosstalk between cytosolic and plastid 2-C-methylerythritol phosphate pathways has been clearly demonstrated. Squalene synthase is the first enzyme of the sterol branch. All subsequent steps occur within the endoplasmic reticulum. In addition to their recognized role in regulating membrane properties, sitosterol, and 24-methylcholesterol modulate a variety of metabolic and ontogenetic events. The enzyme 3-hydroxy-3-methylglutaryl CoA reductase is a rate-limiting step for carbon flux toward sterols and also positively or negatively responds to a depletion of endogenous sterols or accumulation of intermediates. Sterol methyltransferases and acyltransferase participate in sterol homeostasis. A coordinated regulation of the biosynthetic pathways of sterols and some specific lipids may occur during membrane biogenesis.

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“…In phospholipid bilayers, sterols modify the fluid-like microviscosity and reduce the permeability to small molecules (Mukherjee and Maxfield, 2000). Membrane properties such as composition, width, and (a)symmetry can be changed by certain sterol types (Marsan et al, 1996(Marsan et al, , 1998. In model membranes, the ratio and chemical structure of sterols determine phase separation, positive or negative curvature, and even vesicle pinching off from the sterol-rich phase (Bacia et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Structural Sterols Are Involved in Both the Initiation and Tip Growth of Root Hairs inArabidopsis thaliana

et al. 2010

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Structural sterols are abundant in the plasma membrane of root apex cells in Arabidopsis thaliana. They specifically accumulate in trichoblasts during the prebulging and bulge stages and show a polar accumulation in the tip during root hair elongation but are distributed evenly in mature root hairs. Thus, structural sterols may serve as a marker for root hair initiation and growth. In addition, they may predict branching events in mutants with branching root hairs. Structural sterols were detected using the sterol complexing fluorochrome filipin. Application of filipin caused a rapid, concentration-dependent decrease in tip growth. Filipin-complexed sterols accumulated in globular structures that fused to larger FM4-64–positive aggregates in the tip, so-called filipin-induced apical compartments, which were closely associated with the plasma membrane. The plasma membrane appeared malformed and the cytoarchitecture of the tip zone was affected. Trans-Golgi network/early endosomal compartments containing molecular markers, such as small Rab GTPase RabA1d and SNARE Wave line 13 (VTI12), locally accumulated in these filipin-induced apical compartments, while late endosomes, endoplasmic reticulum, mitochondria, plastids, and cytosol were excluded from them. These data suggest that the local distribution and apical accumulation of structural sterols may regulate vesicular trafficking and plasma membrane properties during both initiation and tip growth of root hairs in Arabidopsis.

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Plant sterols: a neutron diffraction study of sitosterol and stigmasterol in soybean phosphatidylcholine membranes

Cited by 21 publications

References 38 publications

Molecular aspects of the interaction between plants sterols and DPPC bilayers

Molecular aspects of the interaction between plants sterols and DPPC bilayers

5 Sterol metabolism and functions in higher plants

Structural Sterols Are Involved in Both the Initiation and Tip Growth of Root Hairs inArabidopsis thaliana

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