Plant-Specific Features of Ribosome Biogenesis

Weis, Benjamin L.; Kovačević, Jelena; Missbach, Sandra; Schleiff, Enrico

doi:10.1016/j.tplants.2015.07.003

Cited by 128 publications

(183 citation statements)

References 106 publications

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“…Although the general scheme of pre-rRNA processing is conserved in eukaryotes, differences do occur in different species in the factors required at specific developmental stages to ensure the successful processing of pre-rRNA (1,3). We suggest that HID2 may represent one of these factors that have plant-specific features.…”

Section: Discussionmentioning

confidence: 87%

“…3), we propose that HID2-rRNA base-pairing is required for HID2 to regulate pre-rRNA processing. In addition, given the disruptions in pre-rRNA processing documented in ribosome biogenesis factor mutants with distinct aberrant prerRNAs (3,7,9), it is conceivable that HID2 may serve as a safeguard responsible for promoting the processing and minimizing the production of aberrant rRNA species in the errorprone processes that require a large set of transacting factors. A complete model of HID2's function has yet to be established in vivo, and, consequently, a number of important questions remain unanswered.…”

Section: Discussionmentioning

confidence: 99%

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Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis

Zhu

Wang

Qin

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Ribosome production in eukaryotes requires the complex and precise coordination of several hundred assembly factors, including many small nucleolar RNAs (snoRNAs). However, at present, the distinct role of key snoRNAs in ribosome biogenesis remains poorly understood in higher plants. Here we report that a previously uncharacterized C (RUGAUGA)/D (CUGA) type snoRNA, HIDDEN TREASURE 2 (HID2), acts as an important regulator of ribosome biogenesis through a snoRNA–rRNA interaction. Nucleolus-localized HID2 is actively expressed in Arabidopsis proliferative tissues, whereas defects in HID2 cause a series of developmental defects reminiscent of ribosomal protein mutants. HID2 associates with the precursor 45S rRNA and promotes the efficiency and accuracy of pre-rRNA processing. Intriguingly, disrupting HID2 in Arabidopsis appears to impair the integrity of 27SB, a key pre-rRNA intermediate that generates 25S and 5.8S rRNA and is known to be vital for the synthesis of the 60S large ribosomal subunit and also produces an imbalanced ribosome profile. Finally, we demonstrate that the antisense-box of HID2 is both functionally essential and highly conserved in eukaryotes. Overall, our study reveals the vital and possibly conserved role of a snoRNA in monitoring the efficiency of pre-rRNA processing during ribosome biogenesis.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis

Zhu

Wang

Qin

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…The accumulation of cytosolic rRNA relative to 311 chloroplast rRNA preceded the resumption of growth and development of Col-0 (Figure 2A-312 B). We concluded that cold acclimation causes an altered balance between cytosolic and 313 chloroplast ribosome biogenesis and therefore represents a condition that is suitable for the 314 analysis of potential cytosolic ribosome biogenesis factors, such as the Arabidopsis REIL 315 proteins , Weis et al, 2015. Cytosolic rRNA accumulation in the reil1-1 316 reil2-1 mutant after exposure to cold was not fully abolished but delayed compared to cold 317 acclimating Col-0 ( Figure 5A-B).…”

Section: Introductionmentioning

confidence: 93%

“…HOMOLOGUE OF YEAST BRX1-1 (BRX1-1, AT3G15460.1) and NUCLEOLIN 2 (NUC2, 568 AT3G18610.1) (Weis et al, 2015). 569…”

Section: C-repeat/dre Binding Factor1/dehydration-responsive Elemenmentioning

confidence: 99%

Plant Temperature Acclimation and Growth Rely on Cytosolic Ribosome Biogenesis Factor Homologs

et al. 2018

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“…The decrease in TE of many ribosomal proteins could also contribute to auxin-related phenotypes, as similar phenotypes have been reported in ribosome mutants. In addition, the phenotypes of hot3-2 are similar to some mutants of plant ribosome biogenesis factors (Weis et al, 2015), and the TE of mRNAs related to ribosome biogenesis is significantly decreased in hot3-2 (Supplemental Data Set 4, "Translation-related genes").…”

Section: The Eif5b1 Allele Hot3-2 Shows Defects In Certain Developmenmentioning

confidence: 90%

Mutations in eIF5B Confer Thermosensitive and Pleiotropic Phenotypes via Translation Defects in Arabidopsis thaliana

et al. 2017

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The conserved eukaryotic translation initiation factor 5B, eIF5B, is a GTPase that acts late in translation initiation. We found that an Arabidopsis thaliana mutant sensitive to hot temperatures 3 (hot3-1), which behaves as the wild type in the absence of stress but is unable to acclimate to high temperature, carries a missense mutation in the eIF5B1 gene (At1g76810), producing a temperature sensitive protein. A more severe, T-DNA insertion allele (hot3-2) causes pleiotropic developmental phenotypes. Surprisingly, Arabidopsis has three other eIF5B genes that do not substitute for eIF5B1; two of these appear to be in the process of pseudogenization. Polysome profiling and RNA-seq analysis of hot3-1 plants show delayed recovery of polysomes after heat stress and reduced translational efficiency (TE) of a subset of stress protective proteins, demonstrating the critical role of translational control early in heat acclimation. Plants carrying the severe hot3-2 allele show decreased TE of auxinregulated, ribosome-related, and electron transport genes, even under optimal growth conditions. The hot3-2 data suggest that disrupting specific eIF5B interactions on the ribosome can, directly or indirectly, differentially affect translation. Thus, modulating eIF5B interactions could be another mechanism of gene-specific translational control.

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Plant-Specific Features of Ribosome Biogenesis

Cited by 128 publications

References 106 publications

Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis

Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis

Plant Temperature Acclimation and Growth Rely on Cytosolic Ribosome Biogenesis Factor Homologs

Mutations in eIF5B Confer Thermosensitive and Pleiotropic Phenotypes via Translation Defects in Arabidopsis thaliana

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