Plant secondary metabolite profiling evidences strain-dependent effect in the Azospirillum–Oryza sativa association

Chamam, Amel; Sanguin, Hervé; Bellvert, Floriant; Meiffren, Guillaume; Comte, Gilles; Wisniewski‐Dyé, Florence; Bertrand, Cédric; Prigent‐Combaret, Claire

doi:10.1016/j.phytochem.2012.11.009

Cited by 143 publications

(129 citation statements)

References 76 publications

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…Indeed, the fact that BEs combined with TSP lead in some cases to higher aboveground biomass suggests that BEs can increase root growth and therefore the volume of soil explored for nutrient uptake. This mechanism has been described in several studies [61][62][63][64] and results from the direct or indirect production of root growth regulators such as auxin.…”

Section: Possible Modes Of Actionmentioning

confidence: 92%

Potential of three microbial bio-effectors to promote maize growth and nutrient acquisition from alternative phosphorous fertilizers in contrasting soils

Thonar

Lekfeldt

Cozzolino

et al. 2017

Chem. Biol. Technol. Agric.

View full text Add to dashboard Cite

Background: Agricultural production is challenged by the limitation of non-renewable resources. Alternative fertilizers are promoted but they often have a lower availability of key macronutrients, especially phosphorus (P). Biological inoculants, the so-called bio-effectors (BEs), may be combined with these fertilizers to improve the nutrient use efficiency. Methods:The goal of this study was to assess the potential of three BEs in combination with alternative fertilizers (e.g., composted manure, biogas digestate, green compost) to promote plant growth and nutrient uptake in soils typical for various European regions. Pot experiments were conducted in Czech Republic, Denmark, Germany, Italy, and Switzerland where the same variety of maize was grown in local soils deficient in P in combination with alternative fertilizers and the same set of BEs (Trichoderma, Pseudomonas, and Bacillus strains). Common guidelines for pot experiment implementation and performance were developed to allow data comparison, and soils were analyzed by the same laboratory.Results: Efficiency of BEs to improve maize growth and nutrient uptake differed strongly according to soil properties and fertilizer combined. Promising results were mostly obtained with BEs in combination with organic fertilizers such as composted animal manures, fresh digestate of organic wastes, and sewage sludge. In only one experiment, the nutrient use efficiency of mineral recycling fertilizers was improved by BE inoculation. Conclusions:These BE effects are to a large extent due to improved root growth and P mobilization via accelerated mineralization.

show abstract

Section: Possible Modes Of Actionmentioning

confidence: 92%

Potential of three microbial bio-effectors to promote maize growth and nutrient acquisition from alternative phosphorous fertilizers in contrasting soils

Thonar

Lekfeldt

Cozzolino

et al. 2017

Chem. Biol. Technol. Agric.

View full text Add to dashboard Cite

show abstract

“…Recently, we compared the response of two rice cultivars to the inoculation of an endophytic and a root surface-colonizing Azospirillum PGPR by both metabolic and transcriptomic analyses. Results highlight strain-dependent responses of rice (Chamam et al 2013;Drogue et al 2014).…”

Section: Introductionmentioning

confidence: 92%

“…Recent studies have demonstrated the differential production of plant secondary metabolites, in particular phenolic compounds, when crops are inoculated with Azospirillum or consortia of cooperative microorganisms (El Zeramny et al 2007;Walker et al 2011Walker et al , 2012Chamam et al 2013;Couillerot et al 2013). Much more information is available concerning plant secondary metabolic responses to bacterial pathogens.…”

Section: Introductionmentioning

confidence: 99%

Differential responses of Oryza sativa secondary metabolism to biotic interactions with cooperative, commensal and phytopathogenic bacteria

Chamam

Wisniewski‐Dyé

Comte

et al. 2015

Planta

Self Cite

View full text Add to dashboard Cite

Profiling of plant secondary metabolite allows to differentiate the different types of ecological interactions established between rice and bacteria. Rice responds to ecologically distinct bacteria by altering its content of flavonoids and hydroxycinnamic acid derivatives. Plants' growth and physiology are strongly influenced by the biotic interactions that plants establish with soil bacterial populations. Plants are able to sense and to respond accordingly to ecologically distinct bacteria, by inducing defense pathways against pathogens to prevent parasitic interactions, and by stimulating the growth of root-associated beneficial or commensal bacteria through root exudation. Plant secondary metabolism is expected to play a major role in this control. However, secondary metabolite responses of a same plant to cooperative, commensal and deleterious bacteria have so far never been compared. The impact of the plant growth-promoting rhizobacteria (PGPR) Azospirillum lipoferum 4B on the secondary metabolite profiles of two Oryza sativa L. cultivars (Cigalon and Nipponbare) was compared to that of a rice pathogen Burkholderia glumae AU6208, the causing agent of bacterial panicle blight and of a commensal environmental bacteria Escherichia coli B6. Root and shoot rice extracts were analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC). Principal component analyses (PCAs) pinpointed discriminant secondary metabolites, which were characterized by mass spectrometry. Direct comparison of metabolic profiles evidenced that each bacterial ecological interaction induced distinct qualitative and quantitative modifications of rice secondary metabolism, by altering the content of numerous flavonoid compounds and hydroxycinnamic acid (HCA) derivatives. Secondary metabolism varied according to the cultivars and the interaction types, demonstrating the relevance of secondary metabolic profiling for studying plant-bacteria biotic interactions.

show abstract

“…Amounts (10 mg) of freeze-dried roots were transferred in 7-ml glass vials and 2.0 ml of LC-MS grade methanol were added, as reported by several research teams as a viable option to analyse secondary metabolites in plant roots [4,21,22]. The sample was sonicated at room temperature for 30 min to favour extraction, and then the residue was allowed to sediment for further 30 min.…”

Section: Extraction Of Metabolitesmentioning

confidence: 99%

Phytochemical profiling of tomato roots following treatments with different microbial inoculants as revealed by IT-TOF mass spectrometry

Nebbioso¹,

Martino

Eltlbany

et al. 2016

Chem. Biol. Technol. Agric.

View full text Add to dashboard Cite

Background: In light of the growing interest for eco-compatible fertilization, tomato plant roots were treated with four different strains of microorganisms (B1-B4) capable of positively affecting plant growth. The methanolic extracts from treated roots were analysed by reverse phase ultra-high-performance liquid chromatography hyphenated with ion trap time-of-flight high-resolution mass spectrometry to compare their metabolites with that of control plants (B0). Results:We found, in both treated and control plants, several primary metabolites, such as fatty acids and coumaric acid, and other compounds associated with secondary metabolism pathways such as that of cyclopentaneoctanoic acid (CPOA) or hydroxyoctadecadienoic acid (HODE), and additional molecules which were not characterizable with the available data. A semiquantitative assessment of all metabolites became the basis for further processing the metabolic results by principal component analysis, which highlighted significant differences in the PC1 and PC2 components. The PC1 was particularly affected by the presence of arachidonic acid, myristic acid, and two unidentified metabolites. It effectively differentiated control plants from all bioeffectors treatments, and, in particular, the B4 treatment from the rest (B1-B3). The PC2 was mainly affected by palmitic acid, heptadecanoic acid, two CPOAs, one HODE and two unidentified metabolites. These metabolites successfully differentiated the B0 control from all the bioeffectors treatments, and, especially, showed a difference between B1 and B2. Conclusions:Our findings suggest that changes in secondary pathways of lipid metabolism may underlie the biostimulation exerted by the four microbial bioeffectors of this study, and that LC-MS coupled by multivariate analysis can easily fingerprint the metabolic alterations induced by bioeffectors in tomato roots.

show abstract

Plant secondary metabolite profiling evidences strain-dependent effect in the Azospirillum–Oryza sativa association

Cited by 143 publications

References 76 publications

Potential of three microbial bio-effectors to promote maize growth and nutrient acquisition from alternative phosphorous fertilizers in contrasting soils

Potential of three microbial bio-effectors to promote maize growth and nutrient acquisition from alternative phosphorous fertilizers in contrasting soils

Differential responses of Oryza sativa secondary metabolism to biotic interactions with cooperative, commensal and phytopathogenic bacteria

Phytochemical profiling of tomato roots following treatments with different microbial inoculants as revealed by IT-TOF mass spectrometry

Contact Info

Product

Resources

About