Plant responses to metals stress: microRNAs in focus

Islam, Waqar; Naveed, Hassan; Idress, Atif; Ishaq, Daha Umar; Kurfi, Binta G.; Zeng, Fang

doi:10.1007/s11356-022-22451-9

Cited by 4 publications

(3 citation statements)

References 142 publications

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“…However, the molecular mechanisms of miRNAs that regulate the development of potato roots have seldom been reported. Recently, small-RNA sequencing technologies have become an effective method to identify and evaluate the expression profiles of miRNAs in many plants [39,40]. In this research, small-RNA high-throughput sequencing was performed to investigate miRNAs in the early root stage and mature root stage of potatoes.…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of Small-RNA and Degradome Sequencing Reveals Role of Novel stu-miR8006 in Regulating Root Development in Solanum tuberosum L.

Duan,

Yang,

Zhang

et al. 2023

Agronomy

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MicroRNAs are a class of endogenous, non-coding, small-RNA molecules with important functions in plant development and stress response processes. Root systems are important because they allow plants to absorb nutrients and water from the soil and are fundamental for anchoring the plant and responding to environmental signals. However, the roles of miRNAs underlying root development remain poorly investigated in potato. In this research, small-RNA sequencing was performed to thoroughly detect underlying miRNAs and their roles in regulating root development between the early root (ER) stage and the mature root (MR) stage of potato roots. A total of 203 known and 137 novel miRNAs were obtained, and 64 differentially expressed miRNAs (DEMs) were identified between the ER and MR stages. The expression patterns of 12 DEMs were also determined via qRT-PCR. In addition, a mixed degradome library was constructed from the ER and the MR stages to identify the targets of the identified miRNAs, and 2400 target genes were verified to be the targets of 131 miRNAs. Based on target annotation, we identified that nine target genes of six DEMs were probably involved in potato root development, and eight targets of six DEMs were validated via 5’-RLM-RACE assays. These targets may participate in root development by regulating cell proliferation, root cultures (PGSC0003DMT400013837), root meristem growth (PGSC0003DMT400079970), root morphogenesis (PGSC0003DMT400040282), post-embryonic root development (PGSC0003DMT400021612), root hair elongation (PGSC0003DMT400034518), cell wall repair (PGSC0003DMT400074930), and auxin polar transport (PGSC0003DMT400079970), and by negatively regulating cell proliferation (PGSC0003DMT400009997) and cell growth (PGSC0003DMT400003464). The qRT-PCR analysis indicated that most miRNAs have opposing expression patterns to their targets. It is widely accepted that potato root development is regulated by miRNAs, among which stu-miR8006-p5-1ss9AT is substantially down-regulated during root development. We show here that the suppression of stu-miR8006-p5-1ss9AT led to an alteration in the potato root architecture and that it targeted auxin induction in the root culture protein 12-encoding gene that is potentially involved in the regulation of root development. In addition, the suppression of stu-miR8006-p5-1ss9AT led to a significant alteration in the potato root architecture. Altogether, our results might provide some useful insights into stu-miR8006-p5-1ss9AT and the crucial role that it plays in potato root development; they could also facilitate the molecular genetic breeding of potato.

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Section: Discussionmentioning

confidence: 99%

Comparative Study of Small-RNA and Degradome Sequencing Reveals Role of Novel stu-miR8006 in Regulating Root Development in Solanum tuberosum L.

Duan,

Yang,

Zhang

et al. 2023

Agronomy

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“…Under normal biological circumstances, plants maintain an equilibrium between ROS generation and elimination. However, under harsh conditions such as drought, cold, salinity, and heat stress, there is an excessive production of ROS that can lead to oxidative damage in plant cells, affecting membrane lipids, proteins, and nucleic acids (Islam et al 2022a;Zhang et al 2022) Islam et al 2022b). miR398-mediated thermotolerance is linked to alterations in ROS levels during heat stress (Liu et al 2017a).…”

Section: Mirnas Reduce Ros Damagementioning

confidence: 99%

“…The cleavage of SOD genes ( CSD1 and CSD2 ) by miR398 is well‐established in response to oxidative stress. Arabidopsis plants possessing the miR398‐resistant CSD2 gene exhibit enhanced resistance to ultraviolet radiation, heavy metals, and other oxidative stresses (Xu et al 2014; Noman et al 2019; Islam et al 2022b). miR398‐mediated thermotolerance is linked to alterations in ROS levels during heat stress (Liu et al 2017a).…”

Section: Mirnas Mechanisms In Temperature Stressmentioning

confidence: 99%

Plant responses to temperature stress modulated by microRNAs

Islam,

Adnan,

Alomran

et al. 2023

Physiologia Plantarum

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Due to the increasing impact of worldwide environmental changes, temperature stress has become a major factor resulting in crop yield losses. The discovery of temperature‐stress‐responsive protein‐coding genes has made significant progress in understanding plants' complex stress response systems involving microRNAs (miRNAs). The miRNAs are triggered by heat or cold, thus confirming their significant functional role in cold or heat tolerance. Such dependable recommendations significantly broaden our understanding of the regulatory role of miRNAs in plant stress responses. This article presents novel perspectives on the substantial roles of plant miRNAs in responding to and acclimatizing to heat and cold stress. It comprehensively elaborates on miRNAs responsive to temperature stress, their regulatory mechanisms, and their targeted functions in plants. Additionally, the article investigates how miRNAs contribute to safeguarding plant reproductive tissues, mitigating damage caused by reactive oxygen species, modulating heat shock proteins, transcription factors, and phytohormones in the context of temperature stress. The conclusion outlines potential avenues for future research, highlighting the utilization of miRNAs and their regulatory functions to develop economically vital crops with enhanced tolerance to temperature stress, thereby ensuring future food security.

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The possibility of nanoclays for water remediation and biofuel synthesis: a review

Abba,

2024

Nanoclay-Based Sustainable Materials

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Plant responses to metals stress: microRNAs in focus

Cited by 4 publications

References 142 publications

Comparative Study of Small-RNA and Degradome Sequencing Reveals Role of Novel stu-miR8006 in Regulating Root Development in Solanum tuberosum L.

Comparative Study of Small-RNA and Degradome Sequencing Reveals Role of Novel stu-miR8006 in Regulating Root Development in Solanum tuberosum L.

Plant responses to temperature stress modulated by microRNAs

The possibility of nanoclays for water remediation and biofuel synthesis: a review

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