Plant response and huanglongbing disease development against heat treatments on ‘Siam Purworejo’ (<i>Citrus nobilis</i>(Lour)) and ‘Nambangan’ (<i>C. maxima</i>(Burm.) Merr.) under field condition

Widyaningsih, Sri; Utami, Sri Nuryani Hidayah; Joko, Tri; Subandiyah, Siti

doi:10.1080/03235408.2018.1544193

Cited by 17 publications

(11 citation statements)

References 37 publications

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“…Such qPCR negative results are typical for hot summer months, as the bacteria clears in the shoots due to high temperatures, which were in the range of 100-120 °F during May-August. Although well-documented, this problem substantially complicates qPCR-based diagnostics of HLB [31][32][33][34] . The Raman readings, however, reported stable signal intensity of 1610 cm −1 band above 1.925 ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Raman Spectroscopy vs Quantitative Polymerase Chain Reaction In Early Stage Huanglongbing Diagnostics

Sanchez

Pant

Mandadi

et al. 2020

Sci Rep

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Raman spectroscopy (RS) is an emerging analytical technique that can be used to develop and deploy precision agriculture. RS allows for confirmatory diagnostic of biotic and abiotic stresses on plants. Specifically, RS can be used for Huanglongbing (HLB) diagnostics on both orange and grapefruit trees, as well as detection and identification of various fungal and viral diseases. The questions that remain to be answered is how early can RS detect and identify the disease and whether RS is more sensitive than qPCR, the "golden standard" in pathogen diagnostics? Using RS and HLB as case study, we monitored healthy (qPCR-negative) in-field grown citrus trees and compared their spectra to the spectra collected from healthy orange and grapefruit trees grown in a greenhouse with restricted insect access and confirmed as HLB free by qPCR. Our result indicated that RS was capable of early prediction of HLB and that nearly all in-field qPCR-negative plants were infected by the disease. Using advanced multivariate statistical analysis, we also showed that qPCR-negative plants exhibited HLB-specific spectral characteristics that can be distinguished from unrelated nutrition deficit characteristics. These results demonstrate that RS is capable of much more sensitive diagnostics of HLB compared to qPCR. Plant diseases can be caused by a variety of different pathogens, such as viruses, fungi and bacteria. Huanglongbing (HLB), or citrus greening, is a devastating disease that plagues citrus trees in Asia, Africa, and more recently the Americas 1. In the United States, HLB is associated with Candidatus Liberibacter asiaticus (CLas), a gram-negative bacterium that inhabits the plant phloem in uneven and variable titers 2,3. Limited knowledge is available on CLas bacterium due to our inability to culture it in the laboratory. The bacterium is transmitted by the highly mobile Asian citrus psyllid (Diaphorina citri) enabling proliferation of HLB on large agricultural areas. The disease is also able to spread by grafting. In late stages, HLB-infected plants have asymmetric mottling on the leaves and lopsided and green fruits. HLB causes fruit drop and poor fruit quality, e.g. smaller size, less juice yield, higher acidity and lower sugar content. Early stages of the disease, however, have minimal to no symptoms and the bacteria can remain undetectable even by quantitative polymerase chain reaction (qPCR) in the trees for several years after initial infection 4. This, wherein, lies a significant challenge in regard to HLB management. Farmers are reluctant to remove trees that have normal, healthy appearances and keep producing fruits. However, if such trees have CLas bacteria, they allow for psyllids to quickly spread this devastating disease over large distances. Therefore, it becomes critical to detect HLB at the early (pre-symptomatic) stage and develop appropriate intervention strategies for HLB containment. In disease diagnostics, the most predominant methods used to confirm a pathogen are PCR and/or enzyme-linked immunosorbent as...

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Section: Resultsmentioning

confidence: 99%

Raman Spectroscopy vs Quantitative Polymerase Chain Reaction In Early Stage Huanglongbing Diagnostics

Sanchez

Pant

Mandadi

et al. 2020

Sci Rep

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“…Merr. di Purworejo, namun L. asiaticus masih bertahan pada tanaman terinfeksi (Widyaningsih et al, 2019). Sementara itu, pemangkasan cabang bergejala, termoterapi uap, peningkatan nutrisi, induksi resistensi, serta aplikasi fitohormon dan antibiotik belum mencapai tingkat pengendalian HLB yang tinggi atau tidak layak secara ekonomi (Bassanezi et al, 2020).…”

Section: Abstrakunclassified

The Effect of Citrus Farming Practices on Huanglongbing (HLB) Disease Severity in Sambas, West Kalimantan

2021

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Beberapa praktik budidaya dilaporkan mempengaruhi kesesuaian agroekosistem bagi perkembangan penyakit tanaman. Namun demikian, praktik-praktik budidaya jeruk yang mempengaruhi perkembangan penyakit huanglongbing (HLB) dan serangga vektornya yaitu kutu loncat jeruk (KLJ) Diaphorina citri belum banyak diketahui. Penelitian ini mempelajari pengaruh praktik budidaya jeruk terhadap intensitas penyakit HLB melalui survei singkat selama dua minggu pada 37 kebun jeruk yang berlokasi di Kabupaten Sambas. Parameter pengamatan adalah intensitas serangan penyakit HLB, kelimpahan KLJ, dan praktik budidaya yang diterapkan. Pengaruh praktik budidaya terhadap intensitas serangan penyakit HLB dianalisis melalui structural equation modeling berbasis partial least squares (SEM-PLS). Hasil penelitian menunjukkan tingkat penggunaan pestisida, kualitas saluran drainase, dan kepadatan tanaman jeruk berpengaruh positif terhadap intensitas serangan penyakit HLB, sedangkan tingkat keanekaragaman vegetasi dan pemberian pupuk kimia berpengaruh negatif terhadap intensitas serangan penyakit HLB. Pengendalian gulma, kualitas tanah, dan pemangkasan pemeliharaan tidak mempengaruhi intensitas HLB. Oleh karena itu, penanaman jeruk sebaiknya menerapkan sistem polikultur, menggunakan beragam varietas, dan jarak tanam tidak terlalu rapat. Pemberian hara yang dapat meningkatkan ketahanan tanaman jeruk terhadap serangan KLJ hendaknya ditingkatkan. Pengendalian hama dan penyakit tanaman disarankan dilakukan secara terpadu sehingga insektisida hanya digunakan ketika populasi hama mencapai ambang ekonomi. Kata kunci: huanglongbing, jeruk, kutu loncat jeruk, praktik budidaya

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“…After 10 days in healing chamber, the rooted grafted and non-grafted tomato plants (control plants) were removed and then planted in field under plastic mulch (Arwiyanto et al 2015). A 30 ml of R. solanacearum inoculum was splashed close to the roots of plants and the bacterial wilt disease intensity was measured every week by calculating the disease intensity (Widyaningsih et al 2019;Solekha et al 2019). The experiment was arranged in a randomized complete block design consisting of 5 treatments and 4 replications with 10 plants within each treatment, and the total number of experimental units was 200 plants (Arwiyanto et al 2018).…”

Section: Grafting and Planting In The Fieldmentioning

confidence: 99%

Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum

et al. 2020

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Abstract. Navitasari L, Joko T, Murti RH, Arwiyanto T. 2020. Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum. Biodiversitas 21: 4888-4895. Bacterial wilt disease caused by Ralstonia solanacearum is a devastating soil-borne vascular disease of tomato leading to a 100% yield loss. One of the alternatives to suppress the infestation of R. solanacearum infestation is the application of grafting techniques, which has been studied and successfully practiced by tomato growers. However, the infestation mode of R. solanacearum and the rhizobacterial community structure in grafted tomato plants are poorly reported. In this study, the rhizobacterial community structure in grafted tomato plants infected by R. solanacearum was investigated. The experiment was conducted on tomato germplasms with the implementation of tube grafting using resistant rootstocks (Amelia from Indonesia, H.7996 from Asian Vegetable Research Development Center/AVRDC) and susceptible scion (Servo from Indonesia). The rhizobacterial community structure was analyzed by metagenomic study under 16S rRNA genes sequencing with a distinct region (16SV3-V4) that was amplified using a specific primer (16SV4: 515F-806R) 5’-GTGCCAGCMGCCGCGGTAA and 5’GGACTACHVHHHTWTCTAAT. The results indicated that the grafted tomato plants and resistant rootstocks that were infected by R. solanacearum showed significantly lower intensity of bacterial wilt disease compared to the susceptible scion. The rhizobacterial community structure in the grafted tomato plants infected by R. solanacearum was indicated by predominant phyla of Proteobacteria, Firmicutes, and Actinobacteria with dominant genera of Pseudomonas and Bacillus. Besides, significant difference was also indicated by species of Geitlerinema sp. in the grafted tomatoes infected by R. solanacearum.

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Plant response and huanglongbing disease development against heat treatments on ‘Siam Purworejo’ (Citrus nobilis(Lour)) and ‘Nambangan’ (C. maxima(Burm.) Merr.) under field condition

Cited by 17 publications

References 37 publications

Raman Spectroscopy vs Quantitative Polymerase Chain Reaction In Early Stage Huanglongbing Diagnostics

Raman Spectroscopy vs Quantitative Polymerase Chain Reaction In Early Stage Huanglongbing Diagnostics

The Effect of Citrus Farming Practices on Huanglongbing (HLB) Disease Severity in Sambas, West Kalimantan

Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum

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