PLANT RESISTANCE TO VIRUSES | Engineered Resistance

Deom, C. M.

doi:10.1006/rwvi.1999.0002

Cited by 3 publications

(4 citation statements)

References 6 publications

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“…The disease agents were obtained from Malawi and Nigeria, two geographically distinct regions of SSA. In most cases, the degree of PDR shows a positive correlation with the level of sequence homology between the transgene and challenge virus (4,14). From the variability studies conducted, the GRAV CP gene is the logical candidate to use for generating transgenic groundnut plants for PDR.…”

Section: Discussionmentioning

confidence: 99%

“…First, expression of GRAV CP in transgenic plants could result in less GRAV available for transmission. This would occur through either gene silencing (RNA-mediated) or CP-mediated resistance (1,4). Second, since the CP of GRAV is required for the encapsidation of the GRV genome and sat-RNA (18,29), the absence of or reduction in GRAV CP would presumably result in diminished packaging of GRV and sat-RNA and, subsequently, reduced aphid transmission.…”

Section: Discussionmentioning

confidence: 99%

“…Pathogen-derived resistance (PDR) (1,4) represents an alternate strategy for controlling GRD through the generation of transgenic groundnut. PDR could potentially be obtained by introducing GRAV or GRV genomic sequences or genes, or sat-RNA-derived sequences that down regulate GRV replication, into suitable groundnut cultivars.…”

mentioning

confidence: 99%

“…PDR could potentially be obtained by introducing GRAV or GRV genomic sequences or genes, or sat-RNA-derived sequences that down regulate GRV replication, into suitable groundnut cultivars. The success of PDR-, RNA-or protein-mediated (1,4) resistance, is highly influenced by the degree of sequence homology between the sequence of the transgene and the challenging virus (4,14).…”

mentioning

confidence: 99%

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Sequence Diversity Within the Three Agents of Groundnut Rosette Disease

Deom¹,

Naidu²,

Chiyembekeza³

et al. 2000

Phytopathology®

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Sequence diversity was examined in the coat protein (CP) gene of Groundnut rosette assistor virus (GRAV), the overlapping open reading frames (ORFs) 3 and 4 of Groundnut rosette virus (GRV), and the satellite RNA (sat-RNA) of GRV obtained from field isolates from Malawi and Nigeria. These three agents cause groundnut rosette disease, a major disease of groundnut in sub-Saharan Africa (SSA). Sequence analysis showed that the GRAV CP gene was highly conserved (97 to 99%) independent of its geographic source. The nucleotide sequence of the overlapping ORFs 3 and 4 of GRV was highly conserved (98 to 100%) from isolates within a geographic region but less conserved (88 to 89%) between isolates from the two distinct geographic regions. Phylogenetic analysis of the overlapping ORFs 3 and 4 show that the GRV isolates cluster according to the geographic region from which they were isolated, indicating that Malawian GRV isolates are distinct from Nigerian GRV isolates. Similarity within the sat-RNA sequences analyzed ranged from 88 to 99%. Phylogenetic analysis also showed clustering within the sat-RNA isolates according to country of origin, as well as within isolates from two distinct regions of Malawi. Because the GRAV CP sequence is highly conserved, independent of the geographic source of the GRAV isolates, the GRAV CP sequence represents the most likely candidate to use for pathogen-derived resistance in groundnut and may provide effective protection against groundnut rosette disease throughout SSA.

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Section: Discussionmentioning

confidence: 99%