Plant regeneration via somatic embryogenesis from in vitro tissue culture in two Tunisian Cucumis melo cultivars Maazoun and Beji

Rhimi, A.; Fadhel, Najeh Ben; Boussaïd, Mohamed

doi:10.1007/s11240-005-9021-y

Cited by 20 publications

(10 citation statements)

References 9 publications

(7 reference statements)

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“…In the present study asynchronous development of somatic embryogenesis have been reported earlier in some melon species like Cucumis melo (Akasaka Kennedy et al 2004;Rhimi et al 2006;Kageyama et al 1991). Several other investigators have been reported somatic embryogenesis and plant regeneration studies from various explants of cucurbitaceous species like Cucurbita pepo and Cucurbita moschata (Noel et al 1992;Usman et al 2011).…”

Section: Effect Of 24-d Alone or In Combination With Bap On Inductiosupporting

confidence: 77%

High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones

Ramakrishna

Shasthree

2016

Physiol Mol Biol Plants

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We report an efficient somatic embryogenesis and plant regeneration system using leaf cultures of Citrullus colocynthis (L.) and assessed the effect of plant growth regulators on the regeneration process. Initially leaf explants were cultured on Murashige and Skoog medium supplemented with different concentrations of auxins viz., 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid, gibberellic acid alone and along with combination of 6-benzylaminopurine. The different forms of calli such as compact, white friable, creamy friable, brownish nodular, green globular and green calli were induced from the leaf explants on MS medium containing different concentrations of auxins and gibberellins. Subsequently initial callus was subcultured at 1.5 mg L -1 BAP ? 1.0 mg L -1 2,4-D which resulted in 25 % somatic embryos from 85 % nodular embryogenic nodular callus that is highest percentage. Similarly the lowest percentage of somatic embryos was recorded at 2.5 mg L -1 BAP ? 0.5 mg L -1 NAA from 55 % embryogenic globular callus i.e., 16 %. High frequency of embryo development takes place at intermittent light when compared with continuous light in the individual subcultures. The cotyledonary embryos were developed into complete platelets on MS medium. In vitro regenerated plantlets were washed to remove the traces of agar and then transferred to sterile vermiculite and sand (2:1) containing pot.

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Section: Effect Of 24-d Alone or In Combination With Bap On Inductiosupporting

confidence: 77%

High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones

Ramakrishna

Shasthree

2016

Physiol Mol Biol Plants

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“…Subsequently, many transgenic melon plants have been produced (Rhimi et al, 2006;Ren et al, 2012). However, the transformation efficiency in melon is not high enough.…”

Section: Discussionmentioning

confidence: 99%

“…Although melon is susceptible to Agrobacterium infection and responsive to regeneration from explants derived from the cotyledon (Dirks and van Buggenum, 1989) and callus (Moreno et al, 1985), the transformation regeneration frequency varies widely among cultivars (Galperin et al, 2003;Akasaka-Kennedy et al, 2004;Rhimi et al, 2006;Hao et al, 2011) and sources of explants (Fang and Grumet, 1990;Guis et al, 2000;Ren et al, 2012). Moreover, in melon transformation, a high frequency of tetraploids and false-positive regenerants are common phenomena.…”

Section: Introductionmentioning

confidence: 99%

An efficient regeneration protocol for Agrobacterium-mediated transformation of melon (Cucumis melo L.)

Zhang¹,

Gao²,

Wang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. An efficient selection and plant regeneration protocol for Agrobacterium-mediated transformation, using cotyledon node zone-stem connection region of melon, has been developed. The new Agrobacterium-mediated transformation methodology, independent of organ culture, used the entire germinated seed as explants. The transformation system was maximized to maintain the integrity of melon itself, thus avoiding the limitations of traditional tissue culture methods. The transformation was carried out under a non-sterile environment. The incorporation of a selectable marker (neomycin phosphotransferase II) into the genome of transgenic plants was confirmed by PCR and Southern blot analyses. The transformation frequency based on the PCR was 13%. Transgenic melon plants were usually detected by PCR in less than 1 month after Agrobacterium inoculation, and seeds could be harvested in 3 months. The growth characteristics and morphology of the transgenic plants were identical to the untransformed wild-type plants. This method would be beneficial for facilitating the characteristics of gene functions and for boosting the manipulation of melon transformation for commercial purposes.

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“…Melon is susceptible to Agrobacterium infection and responds to regeneration from explants derived from both cotyledon (Dirks and van Buggenum, 1989) and callus (Moreno et al, 1985), although the frequency of transformation regeneration varies widely among cultivars (Galperin et al, 2003;Akasaka-Kennedy et al, 2004;Rhimi et al, 2006;Hao et al, 2011;Akbari et al, 2013) and sources of explants (Fang and Grumet, 1990;Gray et al, 1993;Ren et al, 2012Ren et al, , 2013Bezirganoglu et al, 2013;Zhang et al, 2014;Zhang and Luan, 2015). However, the genes responsible for regeneration of melon continue to be poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Transformation of the CmACS-7 gene into melon (Cucumis melo L.) using the pollen-tube pathway

Zhang

Luan

2016

Genet. Mol. Res.

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ABSTRACT. The aim of the present study was to develop a transformation system that may be useful for introducing agronomically and biotechnologically relevant traits into melon. The production of transplanted melon with maternal inheritance of the transgene could solve problems related to outcrossing between genetically modified crops and conventional crops or their wild relatives. By analyzing the main influencing factors systematically, the pollination time was ascertained and the pollen-tube pathway genetic transformation system was optimized. A screening system for resistant seeds from the T 1 generation was established. The transformed seedlings were grown under standard field conditions and selected using a polymerase chain reaction-based analysis. The resistant plants were detected at a rate of 5%. These results indicate that enhanced production hastens the initiation of bisexual flowers, development of mature bisexual flowers, and fruit set in melon. We have established a melon transformation system based on the pollen-tube method.

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Plant regeneration via somatic embryogenesis from in vitro tissue culture in two Tunisian Cucumis melo cultivars Maazoun and Beji

Cited by 20 publications

References 9 publications

High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones

High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones

An efficient regeneration protocol for Agrobacterium-mediated transformation of melon (Cucumis melo L.)

Transformation of the CmACS-7 gene into melon (Cucumis melo L.) using the pollen-tube pathway

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