Plant Regeneration Through Somatic Embryogenesis in Taxus wallichiana

Datta, Mukul Manjari; Jha, Sumita

doi:10.1007/bf03263257

Cited by 8 publications

(2 citation statements)

References 33 publications

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“…The last species from this genus with a published SE protocol is T. wallichiana [ 101 ]. The authors described an efficient procedure for regeneration of T. wallichiana plants from embryogenic cultures derived from zygotic embryos using half-strength WPM culture medium supplemented with BAP and NAA.…”

Section: Somatic Embryogenesis In Cupressalesmentioning

confidence: 99%

Somatic Embryogenesis in Conifers: One Clade to Rule Them All?

Fraga,

Moraes,

Vieira

et al. 2023

Plants

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Somatic embryogenesis (SE) in conifers is usually characterized as a multi-step process starting with the development of proembryogenic cell masses and followed by histodifferentiation, somatic embryo development, maturation, desiccation, and plant regeneration. Our current understanding of conifers’ SE is mainly derived from studies using Pinaceae species as a model. However, the evolutionary relationships between conifers are not clear. Some hypotheses consider conifers as a paraphyletic group and Gnetales as a closely related clade. In this review, we used an integrated approach in order to cover the advances in knowledge on SE in conifers and Gnetales, discussing the state-of-the-art and shedding light on similarities and current bottlenecks. With this approach, we expect to be able to better understand the integration of these clades within current studies on SE. Finally, the points discussed raise an intriguing question: are non-Pinaceae conifers less prone to expressing embryogenic competence and generating somatic embryos as compared to Pinaceae species? The development of fundamental studies focused on this morphogenetic route in the coming years could be the key to finding a higher number of points in common between these species, allowing the success of the SE of one species to positively affect the success of another.

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Section: Somatic Embryogenesis In Cupressalesmentioning

confidence: 99%

Somatic Embryogenesis in Conifers: One Clade to Rule Them All?

Fraga,

Moraes,

Vieira

et al. 2023

Plants

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“…Natural regeneration of the species is constrained, mainly due to vertebrate predation, overstory shading, and strong dormancy of the seeds (Difazio et al 1988;Garcia et al 2000;Mysterud and Østbye 2004). For species conservation, several in vitro culture methods such as stem cutting (Chee 1995a;, embryo culturing (Chee 1994;Zhiri et al 1994;Chang and Yang 1996;Liao et al 2006;Zarek 2007), propagation of plants via shoot organogenesis from cultures derived from zygotic embryos (Chee 1995a;Majada et al 2000;Datta et al 2006), and Taxus regeneration from somatic embryos (Datta and Jha 2008) have been reported. Of these methods, in vitro embryo culture overcomes dormancy of Taxus embryos and rapidly develops germinated embryos.…”

Section: Introductionmentioning

confidence: 97%

Rapid germination and development of Taxus baccata L. by in vitro embryo culture and hydroponic growth of seedlings

Tafreshi

Shariati

Mofid

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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A highly promising procedure to obtain seedlings of Taxus baccata L. has been developed, which involves a combination of in vitro embryo culture and growth under hydroponic conditions. Embryos isolated from freshly collected seeds were 100% sterile, even though the seeds were not treated with acid or soaked in water prior to culturing. The embryo germination level of non-leached seeds was slightly lower (85%) than those leached in running water for 7 d (100%). The leached embryos germinated with extended roots while the non-leached embryos had abnormal shapes. The embryos cultured on media supplemented with an absorbent (PVP or activated charcoal) had extended roots and shoots and were a larger size without any browning, as compared to those grown without the supplement; activated charcoal gave better results. There were no significant differences in germination rates of T. baccata embryos between the media with differing strengths of macronutrients; however, for further development of the shoot, it was necessary to sub-culture the seedlings in MS in the light. To obtain seedlings with longer roots, they had to be maintained in one-half strength MS in darkness. Approximately 90% of the plants survived when grown hydroponically for 2 mo. The surviving plants showed well-extended roots and were a good starting material for genomic, proteomic, and conservational studies as well as Taxol permeabilization investigations.

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Advances in Conifer Somatic Embryogenesis Since Year 2000

Klimaszewska

Hargreaves

Lelu‐Walter³

et al. 2016

Methods in Molecular Biology

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This review compiles research results published over the last 14 years on conifer somatic embryogenesis (SE). Emphasis is placed on the newest findings that affect the response of seed embryos (typical explants) and shoot primordia (rare explants) to the induction of SE and long-term culture of early somatic embryos. Much research in recent years has focused on maturation of somatic embryos, with respect to both yield and quality, as an important stage for the production of a large number of vigorous somatic seedlings. Attempts to scale up somatic embryo production numbers and handling have resulted in a few bioreactor designs, the utility of which may prove beneficial for an industrial application. A few simplified cryopreservation methods for embryonal masses (EM) were developed as a means to ensure cost-efficient long-term storage of genotypes during clonal field testing. Finally, recent long-term studies on the growth of somatic trees in the field, including seed production yield and comparison of seed parameters produced by somatic versus seed-derived trees, are described.

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Plant Regeneration Through Somatic Embryogenesis in Taxus wallichiana

Cited by 8 publications

References 33 publications

Somatic Embryogenesis in Conifers: One Clade to Rule Them All?

Somatic Embryogenesis in Conifers: One Clade to Rule Them All?

Rapid germination and development of Taxus baccata L. by in vitro embryo culture and hydroponic growth of seedlings

Advances in Conifer Somatic Embryogenesis Since Year 2000

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