Plant regeneration through direct somatic embryogenesis from immature zygotic embryos of the medicinal plant, Paris polyphylla Sm.

Raomai, Shiveirou; Kumaria, Suman; Tandon, Pramod

doi:10.1007/s11240-014-0496-2

Cited by 29 publications

(22 citation statements)

References 51 publications

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“…The addition of 1.0 mg L -1 GA 3 to the medium significantly increased the germination of embryos. The beneficial effect of GA 3 on SE germination has been reported for other plant species (Cangahuala Inocente et al 2007;Sivanesan et al 2011;Siddiqui et al 2011;Peńa-Ramírez et al 2011;Raomai et al 2014).…”

Section: Resultsmentioning

confidence: 64%

Somatic embryogenesis of Pelargonium sidoides DC.

Kumar

Moyo

Staden

2015

Plant Cell Tiss Organ Cult

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Pelargonium sidoides is a high value medicinal plant endemic to South Africa and the Lesotho Highlands. Establishing an efficient regeneration system through somatic embryogenesis is necessary for its conservation. An efficient reproducible protocol for in vitro plant regeneration via somatic embryogenesis of Pelargonium sidoides, an important medicinal plant, is reported for the first time. Embryogenic callus was obtained on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg L -1 picloram, 0.5 mg L -1 thidiazuron and 20 mg L -1 glutamine. Different developmental stages of somatic embryos (SEs: globular embryos, heart shaped embryos, torpedo shaped embryos and cotyledon embryo with radicle) were directly obtained and further matured from embryogenic callus by subsequent subculture on the same medium. The highest frequency of somatic embryos (25.89 ± 1.50) was recovered after 6 weeks. Scanning electron microscopic (SEM) analysis revealed the presence of embryogenic cell clusters that formed cotyledonary embryos. Mature somatic embryos germinated and developed into plantlets after 4 weeks on half-strength MS medium. High plant regeneration frequency (94.4 %) was achieved on half-strength MS medium supplemented with 1.0 mg L -1 gibberellic acid. Rooted plantlets were successfully acclimatized in the greenhouse with a survival rate of 90 %. The protocol developed would be helpful in reducing stress on natural habitats, provide a system for germplasm conservation, regeneration of large numbers of high value clonal plants for commercial production, the isolation of bioactive compounds and genetic transformational studies.

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Section: Resultsmentioning

confidence: 64%

Somatic embryogenesis of Pelargonium sidoides DC.

Kumar

Moyo

Staden

2015

Plant Cell Tiss Organ Cult

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“…Using immature zygotic embryos as initial explants, direct somatic embryogenesis has been achieved using agar solidified 0.5× MS media. A good embryogenic response (higher than 30 %) was obtained using hormone-free media (Raomai et al 2014). Moreover, when primary embryos were isolated and cultured on the same media, secondary, morphologically normal, embryos were formed.…”

Section: Other Select Cases Of Medicinal Plantsmentioning

confidence: 96%

“…Moreover, when primary embryos were isolated and cultured on the same media, secondary, morphologically normal, embryos were formed. This response, however, was reduced after few subcultures, but pretreatment with 1 M mannitol for 12 h re-established the embryogenic potential (Raomai et al 2014). Cotyledonary Paris embryos germinated to form entire seedlings, showing shoots, rhizomes, and radicles when they were exposed to 0.5 mg/L GA 3 .…”

Section: Other Select Cases Of Medicinal Plantsmentioning

confidence: 97%

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Application of Somatic Embryogenesis to Secondary Metabolite-Producing Plants

Vázquez‐Flota

Monforte‐González

Miranda-Ham

2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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Plants display an amazing biosynthetic capacity. To date, around 200,000 different chemical compounds have been isolated from them. Only a relatively few of these compounds are common to all plant species, since they are involved in basic or primary cell processes, such as energy metabolism. However, the broader plant chemical diversity corresponds to those compounds showing a restricted distribution among only a few taxonomically related species and which are not involved in primary metabolic pathways. These compounds are called secondary or specialized metabolites and they have important roles in numerous plant-environment interactions. Aside from these functions, secondary metabolites, and the plants bearing them, represent highly regarded commercial products given their pharmaceutical, flavoring, aromatic, coloring, and poisonous properties. In here, we present some selected examples of secondary metabolite-producing plants for which efficient protocols of somatic embryogenesis have been developed. The review covers mainly plants producing fine chemicals, used either in pharmaceutical or food industries. As shown, the development of somatic embryogenesis procedures could respond to two main goals: the genetic transformation of a given plant species, or the massive propagation of selected materials. Furthermore, the use of such protocols for the generation of diversity through indirect embryo formation is also presented.

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“…In addition, in vitro method can also be adopted for multiplication of this species as this technique has proven to be an alternate and effective means of rapid propagation that can facilitate the recovery of Rare Endangered Threatened (RET) Himalayan herbs ( . Recently, in vitro plant regeneration through direct somatic embryogenesis for this species as well as protocol using thin cell layer culture were developed (Raomai et al 2014(Raomai et al , 2015. Propagation by conventional as well as in vitro methods can be employed for mass scale multiplication of quality propagules for systematic cultivation in locations closer to their natural habitats (in alpine and sub-alpine areas) near village clusters.…”

Section: Propagation Cultivation and Market Scenario:-mentioning

confidence: 99%