Plant regeneration of common ash (Fraxinus excelsior L.) by somatic embryogenesis

Capuana, M.; Petrini, G.; Marco, A. Di; Giannini, Raffaello

doi:10.1007/s11627-007-9030-0

Cited by 31 publications

(15 citation statements)

References 29 publications

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“…Choi et al [15] assumed that higher concentrations of 2,4-D might result in a greater possibility of somatic mutation. The use of cytokinins in combination with auxins has been reported to promote callus induction [16]. Similarly, we observed that the addition of 0. )…”

Section: −1supporting

confidence: 79%

An Efficient Method for Plant Regeneration from Calli of <i>Swertia mussotii</i>, an Endangered Medicinal Herb

He¹,

Xu²,

Yang³

et al. 2012

AJPS

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Swertia mussotii Franch is an endangered medicinal plant in the Qinghai-Tibet Plateau. Its regeneration from callus culture is very difficult. In this study, an efficient method for plant regeneration was developed from its calli. The calli derived from young stem explants of S. mussotii were cultured at two types of temperature treatments to test their efficiency of shoot regeneration. When the calli were cultured at variable temperature (VT, 20˚C during the day and 10˚C at night) treatment, the adventitious shoots were formed at each combination tested. However, that did not occur when the calli were cultured at constant temperature (CT, 25˚C) treatment. The best response (53.7%) of plantlet regeneration was obtained on Murashige and Skoog (MS) medium containing 3 mg·l −1 6-benzylaminopurine with 0.5 mg·l −1 α-naphthaleneacetic acid in the VT treatment. The regenerated plantlets were rooted on half-strength MS medium without growth regulators. They flowered in the following subculture. The results indicate that the treatment of day-night temperature difference is a critical factor to callus differentiation in S. mussotii. This protocol can be used for conservation as well as mass propagation of this medicinal plant.

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Section: −1supporting

confidence: 79%

An Efficient Method for Plant Regeneration from Calli of <i>Swertia mussotii</i>, an Endangered Medicinal Herb

He¹,

Xu²,

Yang³

et al. 2012

AJPS

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“…These results were in accordance with Sharma et al, 2011(Sharma et al, 2011. The callus induction was also promoted by the combination of cytokinin and auxins (Capuana et al, 2007). TDZ is also considered to be one of the most active cytokinins for the shoot induction in plant tissue culture (Pelah et al, 2002;Mithila et al, 2003).…”

Section: Discussionsupporting

confidence: 73%

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

Elangomathavan¹,

S²,

Hariharan³

et al. 2017

Int. J. Adv. Res. Biol. Sci

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In this study an effective plant regeneration protocol for multiple shoot bud induction was optimized for the micropropagation of plantlets through indirect organogenesis from leaf explants of C. collinus. The young leaves were cultured on Murashige and Skoog (MS) medium with Thidiazuron (TDZ), 6-Benzylaminopurine (BAP), Kinetin (KIN), 2,4-Dichlorophenoxyacetic acid (2,4-D) and 1-Naphthaleneacetic acid (NAA) at 0.5-3.0 mg/l for callus induction. The induced calli was excised and subcultured on MS medium supplemented with BAP, KIN and TDZ (0.5-4.0 mg/l) in combination with NAA (0.5 mg/l) to find out the optimum hormone concentration for shoot bud induction. For rooting, half strength MS medium with various concentrations (0.5-3.0 mg/l) of Indole 3-acetic acid (IAA), Indole 3-butyric acid (IBA) and NAA was used. Initially, a creamy white callus was formed from the cut ends of leaf explants and gradually these mass of unorganised cells transformed to light green regenerable callus. The maximum callus induction (100%) was observed in MS medium with TDZ at 1.0 mg/l concentration. The highest frequency of organogenesis was observed in the combination of BAP (1.0 mg/l) and NAA (0.5 mg/l) with a mean number of 12.1±1.1 shoots followed by TDZ (1.0 mg/l) and NAA (0.5 mg/l) with 10.2±1.0 shoots per explants. The healthy shoots were rooted best on half strength MS medium supplemented with 0.5mg/l IAA. The well developed plantlets with healthy roots were successfully acclimatized. The development of thriving protocol for the callus induction and regeneration would make possible for the mass multiplication and in vitro production of plantlets with somaclonal variations.

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“…Somatic embryogenesis is generally promoted by auxins, either alone or in combination with cytokinins (Fei et al 2002;Shahana & Gupta 2002;Anthony et al 2004;Shu et al 2005). The use of cytokinins in combination with auxins has been reported to promote somatic embryogenesis induction (Liu et al 2008;Capuana et al 2007). …”

Section: Resultsmentioning

confidence: 99%

High frequency somatic embryogenesis and synthetic seed production of the endangered species Swertia chirayita

Kumar

Chandra

2013

Biologia

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An efficient protocol for plant regeneration through somatic embryogenesis was established from in vivo leaf explants of Swertia chirayita, a critically endangered medicinal herb. The highest frequency (76%) of embryogenic callus was induced on Murashige & Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/L kinetin (Kn) from in vivo leaf explants. Globular somatic embryos were induced and further matured from such embryogenic calli by subsequent culture on the same medium. The highest number of somatic embryos (48.83 ± 4.6) was recovered from embryogenic calli derived from leaf explants after 6 weeks of culture. Synthetic seeds were produced by encapsulating of torpedo stage embryos in sodium alginate (4% W/V) gel, dropped into 100 mM calcium chloride (CaCl2 · 2H2O) solution. The synthetic seeds were germinated on MS medium. The highest frequency of synthetic seed germination (84%) was observed on MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L NAA. Regenerants were successfully acclimatized under ex vitro condition. This is the first report on synthetic seed production of S. chirayita. Application of these protocols would be helpful in reducing stress in natural habitat, and in long-term storage of elite genotypes through synthetic seed production.

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Plant regeneration of common ash (Fraxinus excelsior L.) by somatic embryogenesis

Cited by 31 publications

References 29 publications

An Efficient Method for Plant Regeneration from Calli of <i>Swertia mussotii</i>, an Endangered Medicinal Herb

An Efficient Method for Plant Regeneration from Calli of <i>Swertia mussotii</i>, an Endangered Medicinal Herb

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

High frequency somatic embryogenesis and synthetic seed production of the endangered species Swertia chirayita

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Plant regeneration of common ash (Fraxinus excelsior L.) by somatic embryogenesis

Cited by 31 publications

References 29 publications

An Efficient Method for Plant Regeneration from Calli of &lt;i&gt;Swertia mussotii&lt;/i&gt;, an Endangered Medicinal Herb

An Efficient Method for Plant Regeneration from Calli of &lt;i&gt;Swertia mussotii&lt;/i&gt;, an Endangered Medicinal Herb

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

High frequency somatic embryogenesis and synthetic seed production of the endangered species Swertia chirayita

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Product

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An Efficient Method for Plant Regeneration from Calli of <i>Swertia mussotii</i>, an Endangered Medicinal Herb

An Efficient Method for Plant Regeneration from Calli of <i>Swertia mussotii</i>, an Endangered Medicinal Herb