Plant Regeneration In Vitro from Primary Leaf Nodes of Soybean (Glycine max) Seedlings

Kim, JooHag; LaMotte, Clifford E.; Hack, Ethan

doi:10.1016/s0176-1617(11)81341-6

Cited by 37 publications

(23 citation statements)

References 13 publications

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“…Our results are similar to those of Kim et al (1990) in that, although BAP was required for the development of large shoot pads, higher levels were found inhibitory to shoot elongation. In our study, explant exposed to only 5 μM kinetin developed small shoot pads, but elongated shoots rapidly grew into plantlets.…”

Section: Discussionsupporting

confidence: 89%

“…The meristem cells targeted for transformation are different from those targeted in other transformation methods involving organogenesis, which are the cells located at the cotyledonary node of seedlings (Hinchee et al 1988;Townsend and Thomas 1994), the freshly germinated meristem cells of soybean seeds (Chee et al 1989;Martinell 2002;Khan 2004), and callus tissue (Hong et al 2007). In Kim et al (1990), the attachment of the cotyledon to the explant was advantageous for shoot induction and regeneration (presumably due to the storage of nutrients and hormones in the cotyedon) and, as a result, was included as part of the explant for the first 33 d in the transformation protocol.…”

Section: Discussionmentioning

confidence: 99%

“…To date, regeneration has only been observed through somatic embryogenesis or organogenesis from apical or axillary meristematic cells. Besides direct regeneration from the apical meristem cells, organogenic regeneration has been reported in soybean from or near the axillary meristem cells at or near the cotyledonary node (Kimball and Bingham 1973;Cheng et al 1980;Hinchee et al 1988) and higher nodes (Wright et al 1987b;Kim et al 1990), or arising indirectly from organogenic callus tissue (Wright et al 1987a;Sairam et al 2003). Regeneration via somatic embryogenesis in soybean has been reported using a variety of starting explant materials, including embryonic axes, intact zygotic embryos, and excised cotyledons (Christianson et al 1983;Ranch et al 1985;Lazzeri et al 1985).…”

Section: Introductionmentioning

confidence: 99%

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A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings

Olhoft

Bernal

Grist

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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A novel Agrobacterium rhizogenes-mediated transformation method using a primary-node explant from Dairyland cultivar 93061 was developed for soybean using the disarmed Agrobacterium strain SHA17. Transformed plants regenerated from explants inoculated with SHA17 were fertile and phenotypically normal. In a comparative experiment, regeneration frequencies were not significantly different between explants inoculated with A. rhizogenes strain SHA17 and Agrobacterium tumefaciens strain AGL1

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings

Olhoft

Bernal

Grist

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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“…However, arginine and cysteine did not significantly alter shoot length in comparison to the control. Kim et al (1990) reported that L-proline inclusion in culture media enhanced shoot regeneration from excised primary leaf nodes of soybean. According to Shetty et al (1992), partial substitution of certain amino acids for NH 4 NO 3 in MS medium enhanced organogenesis of soybean seedling explants.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Effects of L-arginine and L-cysteine on growth, and chlorophyll and mineral contents of shoots of the apple rootstock EM 26 cultured in vitro

Sotiropoulos¹,

Dimassi²,

Therios³

2005

Biol. plant.

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1, 5, or 10 mM arginine and 25, 50, or 100 µM cysteine were added in the Murashige and Skoog medium. By increasing arginine concentration the number of shoots per explant increased. Inclusion of 50 µM cysteine in the medium resulted in maximum number of shoots but it was not significantly different in comparison to 10 mM arginine. The chlorophyll content was significantly increased in explants treated with 10 mM arginine in comparison to the control, 1 mM arginine and 25 µM cysteine. By increasing arginine and cysteine concentrations of the medium, N, K, and Ca contents of explants increased but no significant changes in P, Mg, Fe, Mn, Zn, and B contents were observed.

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“…There have been several reports of shoot formation from cotyledonary nodes and plumules , Yang et al 1990), epicotyl-hypocotyl areas (Shetty et al 1992), epicotyls (Wright et al 1987a), primary leaf nodes (Kim et al 1990), and stem nodes ) of 3 to 28-day-old soybean seedlings.…”

Section: Introductionmentioning

confidence: 99%

Adventitious Shoot Formation from Hypocotyl Sections of Mature Soybean Seeds.

Yoshida

2002

Breed. Sci.

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A new system for simple and efficient shoot regeneration of soybean (Glycine max (L.) merr.) is reported using the hypocotyl of mature seeds. Two transverses of 1-mm sections of the hypocotyl were cut from mature seeds: sections containing a cotyledonary node and sections 1 to 2 mm from the cotyledonary node. The effects of thidiazuron (TDZ) concentrations, plating methods, and genotypic differences were examined. Shoots were formed from cotyledonary node ends in all conditions examined. Meanwhile, shoots were formed from the ends of hypocotyl sections on B5 media containing TDZ. The optimal TDZ concentration for organogenesis was 2~10 OM. The efficiency of organogenesis varied with the method of plating of explants. The ends of the hypocotyl sections in contact with the media only produced adventitious shoots. Adventitious shoots were formed effectively by placing explants on the media oriented so that the hypocotyl axes were perpendicular to the surface of the media. The efficiency of organogenesis differed with the position of the hypocotyl ends. It was important to use the ends that were 1 mm from the cotyledonary nodes. Genotypic differences were observed in the organogenesis of the hypocotyl ends.

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Plant Regeneration In Vitro from Primary Leaf Nodes of Soybean (Glycine max) Seedlings

Cited by 37 publications

References 13 publications

A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings

A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings

Effects of L-arginine and L-cysteine on growth, and chlorophyll and mineral contents of shoots of the apple rootstock EM 26 cultured in vitro

Adventitious Shoot Formation from Hypocotyl Sections of Mature Soybean Seeds.

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