Plant Regeneration in Coreopsis lanceolata L. from Leaf Tissue Cultures

Abstract

<jats:p>Excised leaf sections of lance coreopsis cultured on Murashige Skoog (MS) medium produced adventitious shoots in response to BA. When the combinations of 0, 0.5, 1, or 2 μ<jats:sc>m</jats:sc> NAA with 0, 5, 10, 20, or 40 μ<jats:sc>m</jats:sc> BA were tested, shoots were induced by any of the four BA concentrations used in the medium, regardless of the presence of NAA. The average number of shoots formed per leaf section ranged from 1.4 to 4.3 seven weeks after culture initiation. Roots were induced at the base of individual shoots on the same regeneration medium when cultures were kept longer than 7 weeks. The rooted plants were transferred successfully into soil. The regenerated plants had the same growth and flowering characteristics as the seed-grown plants. Chemical names used: benzyladenine (BA); naphthaleneacetic acid (NAA).</jats:p>

Somaclonal Variation of Coreopsis Regenerated from Leaf Explants

“…Cammareri et al (2002) detected somaclonal variation (capitulum and disk diameter, ligulate flower number) in Aster cordifolius L. 'White Elegans' regenerated from leaf explants. The somaclonal variation described for Aster may be applied to Coreopsis, through a protocol established by Lee et al (1994) using leaf explants. The objectives of this study were to evaluate Coreopsis somaclones, and investigate the persistence of somaclonal variation through vegetative and sexual reproductive cycles.…”