Plant regeneration from soybean cotyledonary node segments in culture

Cheng, Tsai-Ying; Saka, Hitoshi; Voqui-Dinh, Thanh H.

doi:10.1016/0304-4211(80)90084-x

Cited by 129 publications

(37 citation statements)

References 21 publications

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“…Although these multiple shoot-buds developed in MS medium without growth regulators, it is often reported that cytokinins such as BAP added singly into the growth medium is required to induce multiple shoot development. Multiple shoot development from embryos has been reported in soybeans (Cheng et al 1980) and almonds (Hisajima 1982). Further research is needed to establish the effects of cytokinins on the development of multiple shoot-buds in P. cynaroides.…”

Section: Discussionmentioning

confidence: 99%

A protocol for direct somatic embryogenesis of Protea cynaroides L. using zygotic embryos and cotyledon tissues

Toit

Reinhardt

2007

Plant Cell Tiss Organ Cult

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We describe a protocol for somatic embryogenesis of Protea cynaroides, with potential for high frequency production of this important horticultural species. Somatic embryos formed directly on both P. cynaroides mature zygotic embryos and excised cotyledons cultured on MS medium without growth regulators. The addition of growth regulators such as naphthalene acetic acid (NAA) (5; 13 and 27 μM) and 2,4-dichlorophenoxyacetic acid (2,4-D) (5; 11 and 23 μM), in combination with thidiazuron (TDZ) (1 μM), benzylaminopurine (BAP) (1 μM) or kinetin (1 μM) suppressed the formation of somatic embryos. After eight weeks in culture, formation of somatic embryos was observed.Zygotic explants formed the most embryos when cultured in a 12-h photoperiod in comparison to explants cultured in the dark. Up to 83% of these embryos germinated after transferal to the germination medium containing 0.3 μM GA 3 . Significantly fewer embryos germinated in MS medium with no growth regulators, or supplemented with higher concentrations of GA 3 , while low germination percentages were also observed in MS media containing casein hydrolysate and coconut water. The germination of normal somatic embryos (two separate cotyledons and a single radicle) was observed only in media containing either no growth regulators, 0.3 μM GA 3 or 1 μM GA 3 . All embryos that germinated in high concentrations of GA 3 were malformed.Abbreviations 2,4D 2,4-Dichlorophenoxyacetic acid -BAP 6-Benzylaminopurine -GA 3 Gibberellic acid -NAA 1-Naphthalene acetic acid -PAR Photosynthetic active radiation -TDZ Thidiazuron

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Section: Discussionmentioning

confidence: 99%

A protocol for direct somatic embryogenesis of Protea cynaroides L. using zygotic embryos and cotyledon tissues

Toit

Reinhardt

2007

Plant Cell Tiss Organ Cult

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“…Besides using immature cotyledons and embryos, a lot of work has also been carried out using cotyledonary node explants from seeds or plantlets after few days of germination. First report of plant regeneration from soybean cotyledonary node segment of seedlings grown in the presence of BAP was by Cheng et al, 1980. They obtained multiple shoot bud formation on medium containing high concentration of BAP but better bud growth was noticed when cultures were transferred to low concentration of BAP.…”

Section: Soybean Tissue Culture Strategiesmentioning

confidence: 99%

“…They further concluded that explant, inoculation medium and appropriate concentration/ combination of growth hormone are also essential for better regeneration efficiency. Stem node segments were also cultured on different basal medium for shoot bud formation (Saka et al, 1980). Combination of MS salts and B5 vitamins supplemented with BAP was found better choice to produce shoot buds.…”

Section: Soybean Tissue Culture Strategiesmentioning

confidence: 99%

Soybean: Plant Manipulation to Agrobacterium Mediated Transformation

Zia¹

2011

Soybean - Genetics and Novel Techniques for Yield Enhancement

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“…In addition to shoot induction, cytokinins are reported to have a stimulatory or inhibitory effect on different developmental processes such as root growth and branching, apical dominance in the shoot, chloroplast development and leaf senescence (Mok and Mok 2001). According to Cheng et al (1980), treatment with BA caused diversion of normal shoot apex development to form multiple shoot development in Glycine max. So far, early events of multiple shoot formation have not been characterized in kenaf.…”

Section: Introductionmentioning

confidence: 99%

Light and Scanning Electron Microscopic Analysis of Benzyl Adenine Induced Multiple Shoot Regeneration in Kenaf (Hibiscus cannabinus L.)

Herath

Suzuki²,

Hattori

2005

Breed. Sci.

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Morphological changes in shoot apices of kenaf treated with benzyl adenine (BA) were compared with those of untreated control plants using light and scanning electron microscopy (SEM). Seven days after the start of BA treatment (day 7), meristematic regions appeared adjacent to the axillary buds, and on the abaxial surface of the primary leaf primordia. Some of these meristematic regions were later developed into shoots. Further, the cells in the primary shoot apical meristem were reprogrammed to induce several meristematic loci. At day 28 in culture, supernumerary vegetative shoot buds were observed in and near the axillary buds. In the control plants neither axillary nor adventitious buds developed. These results suggest that the treatment with BA reprogrammed the developmental fate of a large number of cells in the shoot apex of kenaf. Further, it reconfirmed the ability of BA to overcome the apical dominance of shoots.

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Plant regeneration from soybean cotyledonary node segments in culture

Cited by 129 publications

References 21 publications

A protocol for direct somatic embryogenesis of Protea cynaroides L. using zygotic embryos and cotyledon tissues

A protocol for direct somatic embryogenesis of Protea cynaroides L. using zygotic embryos and cotyledon tissues

Soybean: Plant Manipulation to Agrobacterium Mediated Transformation

Light and Scanning Electron Microscopic Analysis of Benzyl Adenine Induced Multiple Shoot Regeneration in Kenaf (Hibiscus cannabinus L.)

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