Plant regeneration from shoot callus of rosewood (Dalbergia latifolia Roxb.)

Abstract

In vitro propagation of trees using cell, tissue and organ culture is a fast emerging area. We report here the clonal propagation of Indian rosewood (Dalbergia latifolia Roxb.) from shoot callus cultures of 5 year old trees. Bud regeneration was obtained on MS media supplemented with BA and NAA. About 35% of the cultures showed organogenesis. Shoots measuring about 3-5 cm can be excised and rooted in White's medium supplemented with 1-2 mg/L IAA. Rooted plants were successfully established in soil.

Review of Literature

“…Regeneration via adventitious shoots in woody species has been reported in Dalbergia latifolia (Lakshmisita et al 1986), Aegle marmelos (Islam et al 1993), Sesbania grandiflora (Detrez et al 1994), Liquidambar styraciflua (Kim et al 1997), Prunus avium (Hammatt and Grant 1998), D. sissoo (Pradhan et al 1998;Chand et al 2002), Azadirachta indica (Eswara et al 1998;Sharma et al 2002), Salix nigra (Lyyra et al 2006), Paulownia tomentosa (Corredoira et al 2008), Platanus occidentalis (Sun et al 2009), Jatropha curcas (Kumar et al 2010), Cassia angustifolia (Siddique et al 2010),Tabebuia donnell-smithii rose (GonzalezRodriguez et al 2010), Pterocarpus marsupium (Husain et al 2010), and Albizia lebbeck (Perveen et al 2011). …”