Plant regeneration from protoplasts of Japanese lawngrass

Inokuma, Chie; Sugiura, Kiyoyuki; Cho, Chol; Okawara, Ryoji; Kaneko, Seiji

doi:10.1007/s002990050110

Cited by 5 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…By using mature embryos of zoysiagrass in the present study, 97.1% of embryos developed into calli just after 18 days of culture, which was greater than the frequencies of 50–70% in previous researches (Asano et al. 1996; Inokuma et al. 1996; Li et al.…”

Section: Discussioncontrasting

confidence: 67%

“…Mature seeds were also used to induce callus formation on zoysiagrass (Asano et al. 1996; Inokuma et al. 1996) and Zoysia sinica Hancev (Li et al.…”

Section: Discussionmentioning

confidence: 99%

“…For zoysiagrass, while there have been reports of successful establishment of an in vitro culture system (Asano et al. 1996; Inokuma et al. 1996; Dhandapani et al.…”

Section: Introductionmentioning

confidence: 99%

“…The duration of more than 3 months for callus induction was mentioned in experiments using mature seeds of zoysiagrass as explants (Asano et al. 1996; Inokuma et al. 1996; Toyama et al.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonicaSteud.)

2010

View full text Add to dashboard Cite

A rapid and efficient in vitro culture system has been established in zoysiagrass (Zoysia japonica Steud.). Embryos isolated from mature seeds were used to induce callus, which improved callus formation and shortened the culture period. A high concentration of 2,4-dichlorophenoxyacetic acid (5 mg L -1 ) in a combination with low concentration of 6-benzyladenine (0.2 mg L -1 ) was proved to be suitable for callus induction in zoysiagrass. Eight types of calli were observed; friable callus was the most regenerable type. Shoot regeneration efficiency was improved by using 1 mg L -1 of thidiazuron with 1-naphthaleneacetic acid (0.05 mg L -1 ) and gibberellic acid (0.1 mg L -1 ). Zoysiagrass shoots continued to grow in MS medium, and rooted in half-strength MS medium. With this system, it appears the duration of in vitro regeneration could be shortened to 16 weeks for zoysiagrass, i.e. from inoculation of embryos to regeneration of plantlets with shoots and roots.

show abstract

Section: Discussioncontrasting

confidence: 67%

“…Mature seeds were also used to induce callus formation on zoysiagrass (Asano et al. 1996; Inokuma et al. 1996) and Zoysia sinica Hancev (Li et al.…”

Section: Discussionmentioning

confidence: 99%

“…For zoysiagrass, while there have been reports of successful establishment of an in vitro culture system (Asano et al. 1996; Inokuma et al. 1996; Dhandapani et al.…”

Section: Introductionmentioning

confidence: 99%

“…The duration of more than 3 months for callus induction was mentioned in experiments using mature seeds of zoysiagrass as explants (Asano et al. 1996; Inokuma et al. 1996; Toyama et al.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonicaSteud.)

2010

View full text Add to dashboard Cite

show abstract

“…Plant regeneration systems for zoysiagrass have been established from protoplasts (Asano 1989, Khayri et al 1989, Inokuma et al 1996 and embryogenic calluses (Bae et al 2001). Recently, transgenic zoysiagrass plants were produced by polyethylene glycol-mediated (Inokuma et al 1998) and Agrobacterium-mediated transformation (Toyama et al 2003, Ge et al 2006, Zhang et al 2007).…”

Section: Zoysiagrass (1) Characteristics and Recently Released Cultivarsmentioning

confidence: 99%

The Present Status of C<sub>4</sub> Tropical Grasses Breeding and Molecular Approaches

Tsuruta

Shimoda

Kouki³

et al. 2015

JARQ

View full text Add to dashboard Cite

Tropical grasses have been widely utilized as warm-season grasses in the warm temperate zone since the early 20th century because of their high yields as well as for perennial forages in their native tropical areas. The high yield of tropical grasses is mainly due to C4 photosynthesis. However, the soaring demands for animal production sparked by economic development in tropical countries mean genetic improvement of such grasses is urgently needed. Considerable breeding efforts have been made to create and develop new tropical grass cultivars, although direct selection from natural populations remains the main method used for breeding. Cross-breeding has not proliferated due to a lack of genetic information concerning the polyploidy, high sterility, outcrossing, and apomixis of these grasses, although several of these difficulties are starting to be resolved by advanced research using molecular biology tools. These tools are an effective means of achieving genetically improving of tropical grasses, and further development is anticipated. In this review, achievements in the improved guineagrass, brachiariagrass, sorghum, and zoysiagrass are introduced and discussed.

show abstract

Transient gene expression system in zoysiagrass leaf mesophyll protoplasts

Kim

Doan

Lee

et al. 2021

Plant Biotechnol Rep

View full text Add to dashboard Cite

Transient expression of genes in protoplasts is a versatile technique for rapid functional characterization of genes by assessing protein localization or effector-reporter responses. In addition, protoplasts have been widely used for generating transgenic or gene-edited plants in model or crop plants. Zoysiagrass (Zoysia japonica Steud.) is one of the most economically important turf plants used in many living places or natural fields, but its management is labor-intensive. Therefore, genetic manipulation using valuable genes is highly demanded in zoysiagrass. Although transient expression systems in zoysiagrass facilitates the identification of valuable zoysiagrass genes, transient expression use in the zoysiagrass protoplasts is yet to be reported. Here we describe the methodology and feasibility for transient expression of genes in the zoysiagrass protoplasts isolated from green leaves. We obtained more than 70% of transfection efficiency in the zoysiagrass protoplasts using polyethylene glycolmediated transfection. Additionally, we showed the feasibility of cellular, biochemical, and molecular approaches using the zoysiagrass protoplasts transiently expressing genes of interest through fluorescent microscopic observation, immunoblot analysis, and luciferase-based promoter activity assay. Along with the genome draft of the Zoysia family, transient expression will be a valuable tool to rapidly investigate gene functions. An initial assessment of gene function using protoplasts facilitate the identification of valuable genes that can improve the economic value of the Z. japonica or its closely related species by gene manipulation.

show abstract

Plant regeneration from protoplasts of Japanese lawngrass

Cited by 5 publications

References 0 publications

Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonicaSteud.)

Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonicaSteud.)

The Present Status of C<sub>4</sub> Tropical Grasses Breeding and Molecular Approaches

Transient gene expression system in zoysiagrass leaf mesophyll protoplasts

Contact Info

Product

Resources

About