Plant regeneration from embryo-derived tissue cultures of soybeans

Ranch, J. P.; Oglesby, Lynnea; Zieliński, Andrzej; Horsch, Robert B.

doi:10.1007/bf02623299

Cited by 142 publications

(77 citation statements)

References 18 publications

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“…Weak and positive correlation between these phases has also been observed in some research reports (BAILEY et al, 1993, SIMMONDS;DONALDSON, 2000). In the present experiment, these soybean cultivars had marked influence on the induction of somatic embryogenesis as previously described through different protocols (BAILEY et al, 1993;KOMATSUDA;OHYAMA, 1988;KOMATSUDA et al, 1992;MEURER et al, 2001;RANCH et al, 1985;SANTARÉM et al, 1997;SHOEMAKER et al, 1991;SIMMONDS;DONALDSON, 2000).…”

Section: Embryogenesissupporting

confidence: 85%

“…The present responses are also in agreement with these reports because the CD 201 and CD 220, which were efficient during the embryogenesis induction, are clustered in a semi-precocious group of maturation. Otherwise, Ranch et al (1985) and Acta Scientiarum. Agronomy Maringá, v. 33, n. 1, p. 67-74, 2011 Bailey et al (1993) did not find any relationship between maturation groups and somatic embryogenesis.…”

Section: Embryogenesismentioning

confidence: 99%

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Evaluation of soybean cultivars on the embryogenic and organogenic potential

Texeira¹,

Braccini²,

Churata

et al. 2011

Acta Sci. Agron.

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ABSTRACT. The first step in plant transformation using somatic embryogenesis or organogenesis for plant regeneration in vitro has been the identification of genotypes with the best totipotency. Thus, the objective of this experiment was to evaluate the totipotency of ten cultivars and one soybean line from the Coodetec Gene Bank. The somatic embryos developed from immature cotyledons measuring from 4 to 6 mm in length, and the organogenesis was evaluated from mature soybean seeds. The cultivars CD 201, CD 216 and CD 220 were the most promising genotypes to produce somatic embryos, whereas the cultivar CD 217 had the highest quantity of differentiated explants, which represents the best regeneration efficiency for participating in organogenic systems. In the experiment with somatic embryogenesis, time to plant regeneration was ten months, but the plants were regenerated after only four months in the experiment with organogenesis.Keywords: Glycine max, somatic embryogenesis, organogenesis.RESUMO. Avaliação de cultivares de soja quanto ao potencial embriogênico e organogênico. A identificação de genótipos que possuam maior totipotência quando submetidos à embriogênese somática e organogênese é a primeira etapa para a condução de um programa de transformação genética. Este trabalho teve como objetivo avaliar o potencial de embriogênese somática e organogênese em nove cultivares de soja e uma linhagem, do banco de germoplasma da Coodetec. Cotilédones imaturos de soja com 4 a 6 mm de comprimento foram utilizados para produção de embriões somáticos, induzidos com 2,4-D, e sementes maduras de soja foram usadas para avaliação do potencial organogênico. As cultivares CD 201, CD 216 e CD 220 mostram-se promissoras para produção de embriões somáticos, enquanto que para o sistema organogênico a cultivar CD 217 apresentou a maior quantidade de explantes diferenciados e manteve a maior eficiência de regeneração. Para obtenção de uma planta via embriogênese somática foram necessários 10 meses, em virtude do período necessário para obtenção dos explantes. O sistema organogênico se mostrou mais rápido, e plantas completas de soja foram obtidas em quatro meses de cultivo in vitro.Palavras-chave: Glycine max, embriogênese somática, organogênese.

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Section: Embryogenesissupporting

confidence: 85%

Section: Embryogenesismentioning

confidence: 99%

Evaluation of soybean cultivars on the embryogenic and organogenic potential

Texeira¹,

Braccini²,

Churata

et al. 2011

Acta Sci. Agron.

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“…Somatic embryos of soybean are induced from immature cotyledons cultured on medium containing a moderately high concentration of synthetic auxin, either 2,4-dichlorophenoxyacetic acid or a-naphthaleneacetic acid. These embryos are able to generate proliferative embryogenic cultures, and whole plants can be recovered from them through differentiation, maturation, and germination (Lazzeri et al 1985;Ranch et al 1985). Immature somatic embryos can retain regenerative properties for Ͼ1 year, with differentiation and development being readily induced when required (Finer and Nagasawa 1988;Parrott et al 1988).…”

mentioning

confidence: 99%

Application of somatic embryos to rapid and reliable analysis of soybean seed components by RNA interference-mediated gene silencing

Nishizawa

Takagi

Teraishi

et al. 2010

Plant Biotechnology

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“…These embryos are able to generate proliferative embryogenic cultures, and whole plants can be recovered from them through differentiation, maturation, and germination (Lazzeri et al 1985;Ranch et al 1985;Finer and Nagasawa 1988;Parrott et al 1988). Immature somatic embryos can be maintained with regenerative capacity for more than a year, with differentiation and maturation being readily induced when required (Finer and Nagasawa 1988;Parrott et al 1988;Walker and Parrott 2001).…”

mentioning

confidence: 99%

Maturation of somatic embryos as a model for soybean seed development

Nishizawa

Ishimoto

2009

Plant Biotechnology

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Soybean somatic embryos have attracted attention both as a model of zygotic embryos and as explants for the generation of stable transgenic plants. We have now characterized the maturation of soybean somatic embryos in detail by examining both the accumulation of the major seed storage proteins b-conglycinin and glycinin as well as changes in cellular organization. Protein storage vacuoles and oil bodies, which are the main depositories of seed storage reserves, formed within cells during the maturation of somatic embryos. The seed storage proteins were gradually synthesized and accumulated in the protein storage vacuoles in a manner similar to that apparent in seeds. The a and a Ј subunits of bconglycinin were detected earlier than the b subunit of this protein and glycinin. In addition, The a and a Ј subunits of bconglycinin accumulated in both the cotyledons and the hypocotyl of somatic embryos, whereas the b subunit of bconglycinin and glycinin accumulated only in the cotyledons. These temporal and spatial characteristics of storage protein production in maturating somatic embryos are similar to those in developing seeds, although the maturation of somatic embryos ceases prematurely without attainment of the final stages of development. Our findings suggest that somatic embryos are suitable for verification of seed-specific traits such as the biosynthesis of seed components. Key words: Seed development, somatic embryogenesis, soybean (Glycine max [L.] Merr.), storage protein.Plant Biotechnology 26, 543-550 (2009) Original Paper a Present address: National Institute of Crop Science, Tsukuba, Ibaraki 305-8518, Japan. Abbreviations: BSA, bovine serum albumin; PAGE, polyacrylamide gel electrophoresis; PBS, phosphate-buffered saline; PCR, polymerase chain reaction; PSV, protein storage vacuole; RT, reverse transcription; a-TIP, a-tonoplast intrinsic protein. This article can be found at http://www.jspcmb.jp/ Mcmullen 1991; Khalafalla et al. 2005). Transgenic immature somatic embryos can also be maintained in culture, and homogeneous masses of such embryos can be readily and repeatedly subjected to induction of differentiation and maturation. Somatic embryos have therefore been used to screen for transgene expression and to examine transgene effects before recovery of whole plants (Cahoon et al. 1999;Cahoon et al. 2000;Cahoon et al. 2002;Herman et al. 2003;Chen et al. 2006;Kajikawa et al. 2008). In addition to the use of somatic embryos as a provisional screening system, we hypothesized that such embryos could be substituted for seeds for validation of gene function in the synthesis of seed components. Mature somatic embryos of soybean have been found to contain seed storage proteins (Komatsuda et al. 1992;Stejskal and Griga 1995), and the fatty acid composition of such embryos is similar to that of seeds (Shoemaker and Hammond 1988;Dahmer et al. 1991). However, relatively little information has been available regarding common features of somatic embryos and seeds.We have now investigated in detail the...

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Plant regeneration from embryo-derived tissue cultures of soybeans

Cited by 142 publications

References 18 publications

Evaluation of soybean cultivars on the embryogenic and organogenic potential

Evaluation of soybean cultivars on the embryogenic and organogenic potential

Application of somatic embryos to rapid and reliable analysis of soybean seed components by RNA interference-mediated gene silencing

Maturation of somatic embryos as a model for soybean seed development

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