Morphogenesis in Plant Tissue Cultures 1999
DOI: 10.1007/978-94-015-9253-6_2
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Plant Regeneration from Cultured Protoplasts

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Cited by 10 publications
(6 citation statements)
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“…S2). High density of protoplasts or use of feeder cells is generally believed to stimulate cell division, by releasing stimulating substances into the medium (Xu and Xue 1999). However, this needs to be balanced since a high density of viable protoplasts will hamper distinct separation of individually developing calluses.…”
Section: Resultsmentioning
confidence: 99%
“…S2). High density of protoplasts or use of feeder cells is generally believed to stimulate cell division, by releasing stimulating substances into the medium (Xu and Xue 1999). However, this needs to be balanced since a high density of viable protoplasts will hamper distinct separation of individually developing calluses.…”
Section: Resultsmentioning
confidence: 99%
“…Similarly, Schween et al (2003) found that the culture of moss protoplasts (Physcomitrella patens) with high plating density at 3 × 10 5 protoplasts mL -1 sustained viability and promoted the development of cells better than with using a lower density. These results may be due to the fact that high plating density protoplasts are able to release a sufficient amount of the physiologically active compounds, including growth factors and/or amino acids, into the medium to stimulate and sustain mitotic division, normally known as the nurse-effect (Davey et al 2005;Fu et al 2009;Schween et al 2003;Tomar and Dantu 2010;Xu and Xue 1999). In addition, Rákosy-Tican et al (2007) also found that plating efficiency could be improved for all sunflower genotypes and culture protocols (L4 and VKM regeneration protocols) when a low plating density used at 5 × 10 4 protoplasts mL -1 was adjusted to 8 × 10 5 protoplasts mL -1 .…”
Section: Discussionmentioning
confidence: 99%
“…Comparative studies have indicated that protoplasts extracted from petals outperform those derived from young leaves [11]. Furthermore, under identical enzymatic conditions, protoplasts isolated from suspension cultures exhibit the highest yield and vigor, followed by sterile seedling leaves, with guaiac tissues yielding the lowest [12]. It is important to note that the optimal choice of isolation materials and enzymatic digestion time can vary among different plant species, necessitating preliminary testing to determine the most suitable materials.…”
Section: Protoplast Isolation 21 Plant Tissues For Protoplast Isolationmentioning
confidence: 99%