Plant regeneration from cotyledons of Prunus persica, Prunus domestica, and Prunus cerasus

Abstract

Shoots were regenerated from the proximal region of immature cotyledons (with the embryonic axis removed) of Prunus persica (peach) and from the same area in mature cotyledons of P. domestica (plum) and P. cerasus (sour cherry) on MS medium containing (in mg 1 ~) thiamine-HC1, 0.4; nicotinic acid, 0.5; pyridoxine-HCl, 0.5; sucrose, 25 000; and 0.7% agar. The medium was supplemented with 0.0-2.5/~M indole-butyric acid and 5-12.5/~M thidiazuron. Cultures were incubated at 24°C under 16 h photoperiod. Shoots regenerated adventitiously over a broad range of thidiazuron concentrations and 2.5/~M indole-butyric acid in 35 days. The presence of the embryonic axis inhibited the development of shoots. Regenerated shoots of peach and plum were rooted on half-strength MS inorganic semi-solid medium with 2.5-5.0/~M indole-butyric acid. Rooted plants were acclimatized and transferred to the greenhouse.

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

“…Genetic transformation in European plum (Prunus domestica L.) has been established through regeneration of the cotyledon and hypocotyl sections of mature embryos (Mante et al, 1989;Scorza et al, 1994), immature embryos and embryonic axes (Tian et al, 2007a;Srinivasan et al, 2012;Petri et al, 2012: Wang et al, 2013. Medium supplemented with thidiazuron (TDZ) 7.5 μM in combination with indole-3-butyric acid (IBA) 0.25 μM has been used for shoot regeneration, with a transformation effi ciency of 1.2% (Scorza et al, 1994;González-Padilla et al, 2003).…”

“…Shoot regeneration has been reported from the proximal region of cotyledons from immature embryos, hypocotyl segments, longitudinal sections of mature embryos (Mante et al, 1989;Pérez-Clemente et al, 2004) and leaves from shoots cultured in vitro (Gentile et al, 2002).…”

“…Regeneration and transformation protocols for P. cerasus (Mante et al, 1989;Tang et al, 2002;Sink, 2005, 2006) and P. avium (Hammatt and Grant, 1998;Tang et al, 2002;Matt and Jehle, 2005;Feeney et al, 2007) were based on adventitious regeneration using leaves and internodes from plants grown in vitro and hypocotyl slices.…”

Review of Literature

“…Different age of explants may have different levels of endogenous hormones and, therefore, the age of explants would have a critical impact on the regeneration success; these results have been reported in many plants, including Prunus (Mante et al 1989), Lachenalia (Niederwieser and Van Staden 1990), Cydonia oblonga (Baker and Bhatia 1993), Aegle marmelos (Islam et al 1993), Malus (Famiani et al 1994), Cercis canadensis (Distabanjong and Geneve 1997), Morus alba (Thomas 2003), and Sapindus trifoliatus (Asthana et al 2011).…”

Regeneration of Plants fromin VitroCulture of Petioles inPrunus DomesticaLindl (European Plum)

“…Additionally, regeneration using cotyledons has been reported for almond (2) cherry (6). For plum, adventitious shoot regeneration from many explants had also been reported in european plum, such as cotyledons (7,20), hypocotyls (19,27), leaves (3,22,23). Some of these explants had been used in transformation, such as hypocotyls (19,24,27).…”