Plant regeneration from cotyledons and embryonic axes in apple: Sites of reaction and effect of pre-culture in the light

Keulemans, Johan; Witte, K De

doi:10.1007/978-94-011-0467-8_74

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…Regeneration was successful on cotyledons and embryonic axes of 'Gloster' (Keulemans and de Witte 1994), on etiolated internodal explants of 'Royal Gala' (Liu et al 1998), on cotyledons of 'Golden Delicious' (Barbieri and Morini 1987), and on the roots of intact micropropagated plantlets (Malus prunifolia Borkh. var.…”

Section: Shoot Regeneration In Vitro: Organogenesismentioning

confidence: 98%

The role of cytokinins in shoot organogenesis in apple

Magyar-Tábori

Dobránszki

Silva

et al. 2010

Plant Cell Tiss Organ Cult

132

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Effective regeneration in vitro is a necessary precondition for the implementation of different biotechnological approaches in plant breeding. Numerous studies have reported on regeneration from apple somatic tissues, and organogenesis has been proved to be influenced by several factors including mother shoots (genotype, size, type, and age of explant), in vitro conditions (dark period, light intensity, and quality), and others (wounding, orientation of leaf explants). However, one of the most important factors before and during the regeneration process is the type and concentration of cytokinin applied. Thidiazuron and benzyladenine are the most frequently used cytokinins in the regeneration systems, but their efficiency depends on genotype and other factors. Other cytokinins (e.g., zeatin and kinetin) have also been tested in several experiments and they were found in general to be less active. The organogenic ability of explants can also be increased by a properly selected cytokinin pre-treatment. Cytokinins applied in the pre-treatments can influence the leaf structure, which in turn can alter the regeneration capacity of the leaf explant. Interactions between factors of pre-treatments (hormones, light, and culture conditions) and factors of the regeneration phase should be considered. This review brings into focus the role of different cytokinins during in vitro shoot development, discussing their effects on the histology of leaves developed in vitro, and how this affects the subsequent regeneration process.

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Section: Shoot Regeneration In Vitro: Organogenesismentioning

confidence: 98%

The role of cytokinins in shoot organogenesis in apple

Magyar-Tábori

Dobránszki

Silva

et al. 2010

Plant Cell Tiss Organ Cult

132

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show abstract

“…Mesmo aceitando-se em princípio este dogma, é bem conhecido o fato de certos tecidos serem mais favoráveis à regeneração do que outros. A regeneração in vitro a partir de explantes maduros foi conseguida em diversas espécies frutíferas (Leblay et al, 1991). Meristemas também têm sido utilizados para a transformação e regeneração em espécies frutíferas lenhosas (Druart et al, 1998).…”

Section: Shoots Induction In Apical and Axillary Buds Of The Apple Rounclassified

“…A média de brotações por explante foi de 2,14. Keulemans & Dewitte (1994), trabalhando com macieira cv. Gloster, obtiveram uma média de 1,8 e 2,3 brotações por explante, em ápices cotiledonares e cotilédones feridos, respectivamente.…”

Section: Shoots Induction In Apical and Axillary Buds Of The Apple Rounclassified

Indução de brotações em gema apical e axilar do porta-enxerto de macieira 'EM-9' cultivadas in vitro

Schuch

Erig²,

Silva

2003

Rev. Bras. Frutic.

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O trabalho objetivou comparar dois tipos de explantes (gema apical e gema axilar) do porta-enxerto de macieira 'EM-9' e o tempo de permanência destes explantes no meio de cultura de indução (20, 30 e 40 dias), na capacidade de regeneração in vitro de brotações. O meio de cultura de indução de regeneração constituiu-se dos sais de MS, suplementado com mio-inositol (100 mg.L-1), sacarose (30 g.L-1), ágar (6,0 g.L-1), BAP (4,44 µM) e ANA (0,54 µM). Transcorrido o tempo de permanência no meio de cultura de indução de regeneração, os explantes foram transferidos para meio de cultura de multiplicação, de constituição semelhante ao meio de indução, porém sem a presença de auxina. A partir dos resultados obtidos, concluiu-se que as gemas apicais e axilares do porta-enxerto de macieira 'EM-9', não diferem quanto à capacidade de regeneração in vitro de brotações e, o tempo de permanência das gemas no meio de indução de regeneração, apenas mostrou diferenças para o comprimento médio das brotações, obtendo-se o maior valor com 30 dias.

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In Vitro Plant Regeneration of Andrographis Paniculata Nees Using Mature Zygotic Embryonic Explants

Mohammed¹

2017

ARTOAJ

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A rapid method for the large-scale propagation of Andrographis paniculata through in vitro culture of embryonic explants has been developed. Adventitious shoot regeneration from embryonic explants was possible in case of cotyledons and root decapitated embryonic axis among all the tested embryonic explants. High frequency of adventitious shoot formation (?) was observed within 30 days from embryonic explants and shoot induction followed either direct regeneration on MS medium supplemented with BAP or indirect adventitious shoot regeneration in TDZ. BAP was more effective than other cytokinins used for direct adventitious shoot induction from both the embryonic explants of A. panicualata with BAP 1.5mg/L producing 73% shooting and 12.3 shoots per explants while study in cotyledons showed best response in BAP 3.0 mg/L with 52% shooting and 9.3 shoots per explants. While TDZ 1.0mg was effective in indirect shoot regeneration among the different concentrations tested with 26% shoot regeneration from embryonic axis with 3.3 shoots and 76% from cotyledons with 6 shoots per explants. Shoots elongated in MS media with GA 0.5mg/L are effectively rooted in half strength MS media with IBA 0.5mg/L and a high percentage of (82.5%) plants were successfully hardened in soil, sand and vermiculite (1:1:1).

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Plant regeneration from cotyledons and embryonic axes in apple: Sites of reaction and effect of pre-culture in the light

Cited by 3 publications

References 13 publications

The role of cytokinins in shoot organogenesis in apple

The role of cytokinins in shoot organogenesis in apple

Indução de brotações em gema apical e axilar do porta-enxerto de macieira 'EM-9' cultivadas in vitro

In Vitro Plant Regeneration of Andrographis Paniculata Nees Using Mature Zygotic Embryonic Explants

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