Plant regeneration from carrot (Daucus carota L.) anther culture derived embryos

Górecka, Krystyna; Krzyżanowska, D.; Kiszczak, Waldemar; Kowalska, Urszula

doi:10.1007/s11738-009-0332-1

Cited by 15 publications

(9 citation statements)

References 14 publications

(26 reference statements)

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“…However, the majority of embryos were formed after 3-4 weeks of culture. Górecka et al [21] reported that embryos appeared after 2 weeks from the onset of an experiment, and their formation was observed until the third month. In isolated microspore cultures, the appearance of the first structures was recorded after 2 weeks, but they were not yet visible to the naked eye.…”

Section: Discussionmentioning

confidence: 99%

“…Plant regeneration and acclimatization were conducted according to the method described by Górecka et al [21]. When embryos in flasks ( Fig.…”

Section: Regeneration and Acclimatization Of Plantsmentioning

confidence: 99%

“…To achieve better adaptation success, Tyukavin [28] used a hydroponic system, but only 14.8% of plants became acclimatized. To acclimatize carrot plants derived from androgenesis in isolated microspore cultures, Górecka et al [21] used a peat-based substrate, which resulted in 70% plant survival. On the other hand, Li et al [18] Andersen et al [8] conducted a 3-year study of anther cultures with two carrot cultivars and determined the number of chromosomes of the plants in the regenerated population.…”

Section: Stages Of Androgenesismentioning

confidence: 99%

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Comparison of methods for obtaining doubled haploids of carrot

et al. 2017

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Doubled haploid lines of carrot can be obtained through androgenesis in anther cultures and in isolated microspore cultures. The two methods were compared using three carrot cultivars ('Kazan F1' , 'Feria F1' , and 'Narbonne F1') at the androgenesis induction stage, during plant regeneration from embryos, and during acclimatization of androgenetic plants as well as their characterization. It was found that cultivar was the main factor affecting the efficiency at each stage of plant production in both anther and isolated microspore cultures. The efficiency of androgenesis in anther cultures of 'Feria F1' was considerably higher in comparison with isolated microspore cultures, and more plants were obtained from the embryos of androgenesis-cultured plants. In 'Kazan F1' and 'Narbonne F1' , more acclimatized androgenetic plants were produced from anther cultures. Ploidy assessment of acclimatized plants of 'Narbonne F1' showed that the majority of the plants in the population derived from anther cultures had a doubled chromosome (DH) set. On the other hand, the majority of plants obtained from isolated microspore cultures were haploids. When assessing homozygosity, it was found among plants obtained in anther cultures that the percentage of homozygotes for phosphoglucose isomerase (PGI) and aspartate aminotransferase (AAT) depended on the cultivar. In contrast, the majority of plants derived from isolated microspore cultures were homozygous regardless of cultivar.

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Section: Discussionmentioning

confidence: 99%

“…Plant regeneration and acclimatization were conducted according to the method described by Górecka et al [21]. When embryos in flasks ( Fig.…”

Section: Regeneration and Acclimatization Of Plantsmentioning

confidence: 99%

Section: Stages Of Androgenesismentioning

confidence: 99%

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Comparison of methods for obtaining doubled haploids of carrot

et al. 2017

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“…Currently, the list of cultivated species that have originated from haploids derived through in vitro androgenesis includes barley [58], wheat [59], rape [62], carrot [65][66][67], cucumber [68] and cabbage [69]. Production of embryos from female gametophyte cells via apogamy or parthenogenesis ensures stability of DH lines (while retaining a low rate of albinism).…”

Section: Haploids and Double Haploid Plantsmentioning

confidence: 99%

65 years of in vitro culture in Poland

Zenkteler

2013

Acta Soc Bot Pol

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This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications, have been achieved within the areas of meristem culture and micropropagation of valuable genotypes, somaclonal variation, production of haploids and DH plants, somatic embryogenesis, in vitro culture of embryos and ovules, improvement of plant disease resistance, somatic hybridization and transformation of plants. Due to the fact that in vitro domain is a very broad science the authors are aware that this review might not fully cover all the scientists working with tissue culture and their achievements

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“…Medium composition has been identified as a key factor influencing initial callus induction and subsequent plant regeneration in some model plants such as cereal crops and brassica (Keller at al., 1975;Chen et al, 2005;Gorecka et al, 2009). An auxin has been reported to be absolutely required to initiate and promote microspore embryogenesis and a small amount of cytokinin in addition to auxin improves the yield of embryoids in anther culture of oilseed rape (Loh and Ingram, 1982).…”

Section: Introductionmentioning

confidence: 99%

Plant Regeneration from Anther Culture of Panax ginseng

Lee¹,

Khorolragchaa²,

Sun³

et al. 2013

Korean Journal of Plant Resources

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-The research concerned of the regeneration of plants from embryos obtained from anther cultures of ginseng (Panax ginseng C. A. Meyer). The aim was to determine the influence of the regeneration medium on the efficiency of the regeneration process. We conducted to determine the optimum conditions such as cold pretreatment, plant growth regulators and carbon sources on anther culture of P. ginseng. Highest callus formation rate was obtained when flower buds pretreated at 4℃ for 1 day. Among the treated growth regulators with various degrees of concentration in Murashige and Skoog`s (MS) medium, 4.53 μM of 2.4-dichlorophenoxyacetic acid and 4.44 μM of 6-benzylaminopurine gives the most responsive callus with the frequency of 73.89% and 129.53 g of fresh weight. When we used 3-9% of sucrose and maltose among the different kinds and various concentrations of carbohydrates, callus was formed highest 67.29% in the medium with 3% of sucrose. Shoots induced from callus supplemented with 28.9 μM of gibberellic acid and rooted in Gamborg`s B5 medium supplemented with 14.7 μM of indole-3-butyric acid.

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Plant regeneration from carrot (Daucus carota L.) anther culture derived embryos

Cited by 15 publications

References 14 publications

Comparison of methods for obtaining doubled haploids of carrot

Comparison of methods for obtaining doubled haploids of carrot

65 years of in vitro culture in Poland

Plant Regeneration from Anther Culture of Panax ginseng

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