Plant regeneration from callus cultures of salt marsh hay, Spartina patens, and its cellular-based salt tolerance

Li, Xianggan; Seliskar, Denise M.; Moga, Jennifer A.; Gallagher, John L.

doi:10.1016/0304-3770(95)00454-8

Cited by 22 publications

(15 citation statements)

References 12 publications

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“…La misma vía de regeneración de vástagos fue previamente informada para S. alternifl ora, S. cynosuroides y S. patens (Wang et al, 2003;Li et al, 1995Li et al, , 1996. En otras especies, ej.…”

Section: Resultados Y Discusiónunclassified

“…En gramíneas se ha regenerado plantas utilizando embriones inmaduros, infl orescencias inmaduras y cariopses maduros (Dutta Grupta et al, 1999;Echenique et al, 2001 (Li et al, 1995, Li andGallager, 1996;Wang et al, 2003). Sin embargo, en S. argentinensis, la contaminación de sus cariopses por Alternaria alternata, Fusarium graminearum y Bipolaris sorokiniana y otros hongos difi culta la utilización de los mismos para iniciar los cultivos.…”

Section: Introductionunclassified

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Desinfección de cariopses y regeneración de plantas de Spartina argentinensis

2007

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. The highest proportion (20%) of shoot regeneration was achieved with MS media plus 0.25 mg·L -1 of bencyl aminopurine (BAP), whereas 0.5 mg·L -1 naphtalenacetic acid (NAA) was used for root induction. Plants were regenerated via organogenesis. To our knowledge, this is the fi rst report of a protocol for in vitro plant regeneration of S. argentinensis, and it is the initial stage required for obtaining somaclonal variations.

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Section: Resultados Y Discusiónunclassified

Section: Introductionunclassified

Desinfección de cariopses y regeneración de plantas de Spartina argentinensis

2007

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“…The green callus produced on the BND medium proliferated vigorously; therefore, it was maintained on this medium in subsequent subcultures. The morphology of the green callus indicated that it bad the greatest potential for regeneration in this genus (Li et al 1995), and it was selected for further propagation.…”

Section: Callus Induction and Maintenancementioning

confidence: 99%

“…Attempts to simplify the regeneration technique for S. cynosuroides were successful, resulting in a less complicated protocol for this species than lbr S. patens (Li et al 1995). In the simplified protocol, callus induced on ADM medium was maintained on BND medium.…”

Section: Shoot and Root Regenerationmentioning

confidence: 99%

“…Adventitious roots then form from the base of the mature shoots on root regeneration media. Mature callus cultures of S. patens (Li et al 1995) and S. cynosuroides develop convoluted green areas containing shoot meristems, as well as areas of white masses (Figures 1 and 4). Shoot regeneration of S. patens from the green spots is initiated when the auxin concentration of the media is decreased and high levels of cytokinin added.…”

Section: Comparison With Other Wetlands Plantsmentioning

confidence: 99%

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Tissue culture and plant regeneration of big cordgrass,Spartina cynosuroides: implications for wetland restoration

Gallagher

1996

Wetlands

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Big cordgrass, Spartina cynosuroides (Poaceae), grows in extensive stands in brackish marshes along the Atlantic and Gulf coasts of the United States and along the margins of tidal streams where freshwater wetland plants dominate the back marsh. Since big cordgrass is a species of ecological importance in wetland replenishment, a tissue culture and regeneration protocol was developed t'or it. This protocol provides a mechanism for producing improved varieties. Spartina cynosuroides callus was initiated from the mesocotyl of sterile seedlings grown on both a medium containing Murashige and Skoog salts + 3% sucrose + 0.5 mg 1 -~ 6-benzylaminopurine + 1 mg I -~ 1-Naphthaleneacetic acid + 0.5 mg I ~ 2,4-diehlorophenoxyacetic acid and 5% coconut water and a medium containing Murashige and Skoog salts + 3% sucrose + 1 nag 1-~ lndole-3-acetic acid and 1 mg 1-' 2,4-dichlorophenoxyacetic acid, but not from seedlings germinated on the prcvious medium plus 5% coconut water. Shoots formed from callus after it was transferred to a shoot regeneration medium containing Murashige and Skoog salts plus 1.0 mg l '~ 6-benzytaminopurine. Roots tbrmed from the base of these shoots after the larger shoots were transferred to root regeneration medium containing reduced-strength Murashige and Skoog medium. The mode of plant regeneration was via organogenesis, and the regenerated plants have set viable seeds in the greenhouse. This callus culture and regeneration procedure will be useful for producing S. cynosuroides somaclonat variants with superior characteristics for wetland restoration and creation in stressful habitats.

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Cellular Responses To Salinity Of Two Coastal Halophytes With Different Whole Plant Tolerance: Kosteletzkya Virginica (L.) Presl. And Sporobolus Virginicus (L.) Kunth

Seliskar

Gallagher

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Plant regeneration from callus cultures of salt marsh hay, Spartina patens, and its cellular-based salt tolerance

Cited by 22 publications

References 12 publications

Desinfección de cariopses y regeneración de plantas de Spartina argentinensis

Desinfección de cariopses y regeneración de plantas de Spartina argentinensis

Tissue culture and plant regeneration of big cordgrass,Spartina cynosuroides: implications for wetland restoration

Cellular Responses To Salinity Of Two Coastal Halophytes With Different Whole Plant Tolerance: Kosteletzkya Virginica (L.) Presl. And Sporobolus Virginicus (L.) Kunth

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