Plant regeneration by organogenesis in Glycine max

Abstract

A procedure for the regeneration of fertile plants by organogenesis from tissue cultures of soybeans, Glycine max is described. Seeds were germinated on reduced inorganic salt MS medium containing 5μM BA. Cotyledonary nodes were excised and cultured on the same medium. Presence of BA in the medium during seed germination and culture of nodal explants was required for multiple shoot and shoot-bud formation. Histological analyses established the de novo nature of shoot regeneration. Separate reduction of the concentration of inorganic salts or substitution of sucrose for fructose during culture had minimal effects on the regeneration response. Conversely, if the BA was reduced, the inhibition response could not be overcome by increased salt concentration or altered carbon source.

Plant Regeneration via Adventitious Shoot Formation in Platycodon grandiflorum (Jacq. A. DC.)

“…1E, F (Han et al, 2014;Ko 1999;Ko et al 1993). 이는 많은 작물의 신초발생 연구에서 BA가 매우 효과적이다는 결과와 일치하고 있다(Malik and Barwale et al, 1986;Wright et al, 1986). …”

Soybean: Plant Manipulation to Agrobacterium Mediated Transformation

“…Carbon source (sucrose or fructose) and salt concentration (full MS, ½ MS or 1/4MS) have different effects even hormone concentration is kept constant. They further reported that seedlings germinated on water agar medium were not so responsive for shoot induction (Wright et al, 1986). BAP treatment to embryonic axes does not allow the cell to remain quiescent and cells are reprogrammed to produce multiple somatic foci (Buising et al, 1994).…”

Direct introduction of DNA into embryonic axes of Glycine max by microprojectile bombardment

“…Dramatic cultivar-dependent differences in the number of shoots formed per axis were noted by Barwale et al (1986a;. Wright et al, (1986aWright et al, ( , 1986b characterized de novo multiple shoot production from excised cotyledonary nodes during the period of 6 to 28 days after continuous culture on 5 |iM BAP followed on occasion by transfer to 1 jO-M BAP; proregenerative tissues were produced by 6 days. In addition, Wright et al, (1986a; and Barwale et al, (1986a; reported that BAP interfered with subsequent development and elongation of multiple shoots.…”

“…Wright et al, (1986aWright et al, ( , 1986b characterized de novo multiple shoot production from excised cotyledonary nodes during the period of 6 to 28 days after continuous culture on 5 |iM BAP followed on occasion by transfer to 1 jO-M BAP; proregenerative tissues were produced by 6 days. In addition, Wright et al, (1986a; and Barwale et al, (1986a; reported that BAP interfered with subsequent development and elongation of multiple shoots. Wright et al (1986a) concluded, however, that continuous exposure to BAP was required for additional bud production.…”

“…In addition, Wright et al, (1986a; and Barwale et al, (1986a; reported that BAP interfered with subsequent development and elongation of multiple shoots. Wright et al (1986a) concluded, however, that continuous exposure to BAP was required for additional bud production. They also showed histologically that basal regions of cotyledons and epicotyls developed meristematic regions (histologically defined as densely staining) adjacent to the axillary bud.…”