Plant regeneration and thiophene formation in tissue cultures of Tagetes mendocina

Abstract

Plants of the wild species Tagetes rnendocina were regenerated, via organogenesis, from explants and callus cultures, and the occurrence of biocidal metabolites, thiophenes, by such undifferentiated tissues and regenerated plants was investigated. Differences between explant sources were apparent in terms of their competence for dedifferentiation and subsequent organogenesis. Leaf-derived cultures were more responsive than the root-derived ones. Biochemical studies revealed that the plants produced were capable of synthezising thiophene like-compounds following in vitro regeneration.

In vitro callus induction in inflorescence segments of medicinally important endangered plant Rauwolfia serpentina (L.) Benth. ex Kurz – a step towards ex situ conservation

“…2a) as it yields a high contents of secondary metabolites during differentiation (Benavides and Caso, 1993;Maheshwari et al, 2007). The callus retains the capacity to synthesize alkaloid production (Yamamoto and Yamada, 1986;Prem et al, 2002;Anitha and Kumari, 2006).…”

Comparison of seed-derived with micropropagated male-sterile plants ofTagetes erectaL. for F1hybrid seed production

“…Micropropagation of some Tagetes species have been reported (Ram and Mehta, 1982;Kothari and Chandra, 1984;Mukundan and Hjortso, 1991;Benavides and Caso, 1993;Mohamed et al, 2000;Pratibha et al, 2000). However, information on the field performance of Comparison of seed-derived with micropropagated male-sterile plants of Tagetes erecta L. for F 1 hybrid seed production By AJIT KUMAR 1 , S.K.…”

In vitro regeneration technique in Rauwolfia serpentina and quantification of reserpine

“…(Yoshimatsu and Shimomura, 1991) [18] . Sometimes, higher production capacity of producing secondary metabolites observed in callus culture (Benavides and Caso, 1993) [1] and the callus culture can also efficient and reliable to produce alkaloids at high mass (Yamamoto and Yamada, 1986). The methanolic extraction of alkaloids is also reliable in case of callus culture (Kirillova et al, 2001) [6] .…”