Plant Recombinant Human Collagen Type I Hydrogels for Corneal Regeneration

Haagdorens, Michel; Edin, Joel; Groleau, Marc; Shtein, Zvi; Ulčinas, Artūras; Yaari, Amit; Samanta, Ayan; Cėpla, Vytautas; Liszka, Aneta; Tassignon, Marie-José; Simpson, Fiona; Shoseyov, Oded; Valiokas, Ramūnas; Pintelon, Isabel; Ljunggren, Monika Kozak; Griffith, May

doi:10.1007/s40883-021-00220-3

Cited by 15 publications

(25 citation statements)

References 42 publications

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“…Similar strategies are reported for the expression of human collagen type III in the insects and yeast cells [86]. The recombinant human collagen type I (RHCI) derived from tobacco plants have has been used to fabricate the hydrogels for grafting corneal implants [21].…”

Section: Sustainable Production Of Collagen Using Synthetic Biology Platformmentioning

confidence: 94%

“…Various synthetic (non-native) sources of collagen have been developed using synthetic biology tools to produce contaminant-free animal-derived collagen [20,21]. These recombinant sources are devoid of heterogeneity across species, pathogen transfer and immunogenicity.…”

Section: Synthetic Collagenmentioning

confidence: 99%

“…However, due to the faster growth rate and ease of genetic manipulations of yeast expression systems, multiple companies have adopted it as a host for industrial-scale production (Table 2). The other expression systems include plants [21], insects, (isolated) cultured insect cells [46], mammals, and cultured human cells [49]. The transduction of collagen encoding genes in the human cell line is more promising compared to that of non-mammalian cells due to its post-translational modification capacity.…”

Section: Synthetic Collagenmentioning

confidence: 99%

“…In recent years, the synthesis of recombinant collagen is widely experimented in various expression systems including bacteria [83], yeast [84], insects [85], plants [21] and animal cell cultures. The coexpression of type III human collagen in bacteria with prolyl and lysyl hydroxylases have has shown to yield up to 90 mg of collagen per litre [83].…”

Section: Sustainable Production Of Collagen Using Synthetic Biology Platformmentioning

confidence: 99%

“…In the current review, we attempt to summarize the current advances in collagen production from different sources using novel expression systems like plants and algae with industrial consideration. Plant-derived collagen has been reported to be a safe alternative to allografts, animal collagen or recombinant collagen derived from yeast [21] with the advantage of the limitless supply. However, there is a scarcity of literature about the novel expression system for recombinant collagen production using such platforms.…”

Section: Introductionmentioning

confidence: 99%

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A Guide to Collagen Sources, Applications and Current Advancements

Bhadra

Sakpal

Patil

et al. 2021

Systematic Bioscience and Engineering

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The increase in use of collagen products in almost every sector of utility has stipulated the rising demand for collagen. It is an essential fibrous glycoprotein, which is abundantly present in the extracellular matrix and connective tissues in many living organisms. Owing to its unique characteristics such as biocompatibility, bioavailability and weak antigenicity it has gained major interest in the food, pharmaceutical, cosmetic, biomedical and leather industries. The collagen-based composites possess an enhanced capacity to dissipate mechanical energy, strength and stiffness making it the most promising biomolecule for its multifaceted applications. Recently, synthetic biology platforms are getting wide attention for the production of non-native collagen alternatives to meet the rising demand for collagen worldwide. In the initial part, this review aims to explain different sources, structures, biosynthesis of collagen and its types followed by the emerging applications of collagen as a next-generation biomaterial in the later part. The article emphasises the synthesis of non-native collagen using novel expression systems like plant and algae in addition to bacteria and yeast with industrial consideration. In the conclusion section, challenges and opportunities of using synthetic biology tools are described.

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Section: Sustainable Production Of Collagen Using Synthetic Biology Platformmentioning

confidence: 94%

Section: Synthetic Collagenmentioning

confidence: 99%

Section: Synthetic Collagenmentioning

confidence: 99%

Section: Sustainable Production Of Collagen Using Synthetic Biology Platformmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Guide to Collagen Sources, Applications and Current Advancements

Bhadra

Sakpal

Patil

et al. 2021

Systematic Bioscience and Engineering

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Structure and properties of the acellular porcine cornea irradiated with ⁶⁰Co‐γ and electron beam and its histocompatibility

Li,

Sun,

Zhao

et al. 2023

J Biomedical Materials Res

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Acellular porcine cornea (APC) has been used in corneal transplantation and treatment of the corneal diseases. Sterilization is a key step before the application of graft, and irradiation is one of the most commonly used methods. In this paper, APC was prepared by the physical freeze‐thawing combined with biological enzymes, and the effects of the electron beam (E‐beam) and cobalt 60 (60Co‐γ) at the dose of 15 kGy on the physicochemical properties, structure, immunogenicity, and biocompatibility of the APC were investigated. After decellularization, the residual DNA was 20.86 ± 1.02 ng/mg, and the α‐Gal clearance rate was more than 99%. Irradiation, especially the 60Co‐γ, reduced the cornea's transmittance, elastic modulus, enzymatic hydrolysis rate, swelling ratio, and cross‐linking degree. Meanwhile, the diameter and spacing of the collagen fibers increased. In the rat subcutaneous implantation, many inflammatory cells appeared in the unirradiated APC, while the irradiated had good histocompatibility, but the degradation was faster. The lamellar keratoplasty in rabbits indicated that compared to the E‐beam, the 60Co‐γ damaged the chemical bond of collagen to a larger extent, reduced the content of GAGs, and prolonged the complete epithelization of the grafts. The corneal edema was more serious within 1 month after the surgery. After 2 months, the thickness of the APC with the two irradiation methods tended to be stable, but that in the 60Co‐γ group became thinner. The pathological results showed that the collagen structure was looser and the pores were larger, indicating the 60Co‐γ had a more extensive effect on the APC than the E‐beam at 15 kGy.

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