Plant Proteomic Research 4.0: Frontiers in Stress Resilience

Chen, Sixue; Komatsu, Setsuko

doi:10.3390/ijms222413362

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…Novel genes were annotated in the following databases: NR (NCBI non-redundant protein sequences), NT (NCBI non-redundant nucleotide sequences), Pfam (Protein family), KOG/COG (Clusters of Orthologous Groups of proteins), Swiss-Prot (a manually annotated and reviewed protein sequence database), KO (KEGG Ortholog database), and GO (pannzer2 database) [48,49]. TFs were predicted using the plantTF database from https://planttfdb.gao-lab.org (accessed on 15 December 2023) and online software Plant-PAN 4.0 [50,51].…”

Section: Gene Functional Annotationmentioning

confidence: 99%

Integrated Untargeted Metabolome, Full-Length Sequencing and Transcriptome Analyses Reveal the Mechanism of Flavonoid Biosynthesis in Blueberry (Vaccinium spp.) Fruit

Tian,

Liu,

Chen

et al. 2024

IJMS

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As a highly economic berry fruit crop, blueberry is enjoyed by most people and has various potential health benefits, many of which are attributed to the relatively high concentrations of flavonoids. To obtain more accurate and comprehensive transcripts, the full-length transcriptome of half-highbush blueberry (Vaccinium corymbosum/angustifolium cultivar Northland) obtained using single molecule real-time and next-generation sequencing technologies was reported for the first time. Overall, 147,569 consensus transcripts (average length, 2738 bp; N50, 3176 bp) were obtained. After quality control steps, 63,425 high-quality isoforms were obtained and 5030 novel genes, 3002 long non-coding RNAs, 3946 transcription factor genes (TFs), 30,540 alternative splicing events, and 2285 fusion gene pairs were identified. To better explore the molecular mechanism of flavonoid biosynthesis in mature blueberry fruit, an integrative analysis of the metabolome and transcriptome was performed on the exocarp, sarcocarp, and seed. A relatively complete biosynthesis pathway map of phenylpropanoids, flavonoids, and proanthocyanins in blueberry was constructed. The results of the joint analysis showed that the 228 functional genes and 42 TFs regulated 78 differentially expressed metabolites within the biosynthesis pathway of phenylpropanoids/flavonoids. O2PLS analysis results showed that the key metabolites differentially accumulated in blueberry fruit tissues were albireodelphin, delphinidin 3,5-diglucoside, delphinidin 3-O-rutinoside, and delphinidin 3-O-sophoroside, and 10 structural genes (4 Vc4CLs, 3 VcBZ1s, 1 VcUGT75C1, 1 VcAT, and 1 VcUGAT), 4 transporter genes (1 VcGSTF and 3 VcMATEs), and 10 TFs (1 VcMYB, 2 VcbHLHs, 4 VcWD40s, and 3 VcNACs) exhibited strong correlations with 4 delphinidin glycosides. These findings provide insights into the molecular mechanisms of flavonoid biosynthesis and accumulation in blueberry fruit.

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Section: Gene Functional Annotationmentioning

confidence: 99%

Integrated Untargeted Metabolome, Full-Length Sequencing and Transcriptome Analyses Reveal the Mechanism of Flavonoid Biosynthesis in Blueberry (Vaccinium spp.) Fruit

Tian,

Liu,

Chen

et al. 2024

IJMS

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“…The forthcoming World Athletics Championships (Tokyo September 2025) and the Summer Olympic and Paralympic Games (Los Angeles July 2028) will be held in hot summer climates. For context, the hottest temperature recorded on earth (54.4 °C; July 2021) was reported in Death Valley, California, less than 200 miles Extended author information available on the last page of the article (~322 km) from the Olympic and Paralympic site [23]. Elite athletes will inevitably be faced with extreme heat at future events given the increased frequency, length and severity of heatwaves seen and forecast.…”

Section: Introductionmentioning

confidence: 99%

A Review of Elite Athlete Evidence-Based Knowledge and Preparation for Competing in the Heat

Esh,

Carter,

Galan-Lopez

et al. 2024

J. of SCI. IN SPORT AND EXERCISE

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Elite athletes will compete in extreme heat more frequently as global land and sea temperatures increase, alongside more intense, frequent and longer duration heatwaves. Best practices to protect athlete health and performance during competition include heat acclimation/acclimatisation [(HA); i.e., long-term pre-competition preparation] complemented by pre-planned and practised cooling and hydration strategies (i.e., short-term interventions immediately before or during competition). This review explores elite athletes’ current behaviours and practices when preparing for competition in the heat and assesses the level of knowledge that has been exhibited by athletes and their practitioners in this space. Recommendations for future research, discussions of current best practices, and methods to improve translation of research into practice are provided. Available research focuses on small samples of elite endurance athletes during a selection of World Championship/Olympic/Paralympic events (~6% of competing athletes). While generally an increase in the adoption of evidence-based HA is seen chronologically from 2015 onwards, universal adoption is not seen. HA adoption is lowest in those who live/train in cold/temperate environments with cost and access to facilities/equipment being the most commonly reported barriers. Further research is required across the sporting landscape to fully characterise elite athlete behaviours and practices in these spaces. International federations and national governing bodies should continue their efforts to educate athletes and focus on regularly updated and reinvigorated release of evidence-based guidelines (in multiple germane languages) for competing in the heat, to increase the adoption of HA and other heat related best practice.

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Plant Proteomic Research 4.0: Frontiers in Stress Resilience

Abstract: Large-scale high-throughput multi-omics technologies are indispensable components of systems biology in terms of discovering and defining parts of the system [...]

Cited by 2 publications

References 19 publications

Integrated Untargeted Metabolome, Full-Length Sequencing and Transcriptome Analyses Reveal the Mechanism of Flavonoid Biosynthesis in Blueberry (Vaccinium spp.) Fruit

Integrated Untargeted Metabolome, Full-Length Sequencing and Transcriptome Analyses Reveal the Mechanism of Flavonoid Biosynthesis in Blueberry (Vaccinium spp.) Fruit

A Review of Elite Athlete Evidence-Based Knowledge and Preparation for Competing in the Heat

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