Plant Protein Disorder: Spatial Regulation, Broad Specificity, Switch of Signaling and Physiological Status

Hsiao, An‐Shan

doi:10.3389/fpls.2022.904446

Cited by 8 publications

(6 citation statements)

References 69 publications

(85 reference statements)

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“…In recent years, Intrinsically Disordered Proteins (IDPs) have emerged as key players in stress response and plant development (Hsiao, 2022; Hsiao et al, 2020; Zamora-Briseño et al, 2021). After hyperosmolarity or heat stress, a new homeostatic set point is rapidly obtained by establishing an appropriate set of IDP in fibroblasts (Watson et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

The viscoelastic properties ofNicotiana tabacumBY-2 suspension cell lines adapted to high osmolarity

Skrzypczak,

Pochylski,

Rapp

et al. 2024

Preprint

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Plant cells have to maintain the cellular microenvironment within a certain range of its properties to stay alive and grow. The functioning of the cell requires a continuous exchange of mass and energy with an environment. Such system openness could lead to perturbation in cellular microenvironment and disorder in molecular processes. Hyperosmolarity significantly stresses cells, induces ROS, promotes water efflux, and decreases turgor, optionally up to plasmolysis and a cell's shrinkage. However, in time upon exposure to osmotic stress a cellular adaptation could be developed. How the biomechanical balance of the cell is established and maintained in an environment of osmotic stress remains a nurturing question. We show that changes of the viscoelastic properties of protoplasts are element of such adaptation. We used Brillouin light scattering (BLS) and BODIPY-based fluorescence molecular rotors to study Nicotiana tabacum suspension BY-2 cells adapted to high concentrations of NaCl and mannitol. We revealed an increased molecular crowd and viscoelasticity in adapted BY-2 cells, as well as an altered plasma membrane tension. Importantly, viscosity-related changes imposed on adapted BY-2 cells by salt stress were smaller than those observed for control cells. Cellular microenvironments were elucidated in on the background of their own environments, what revealed that alterations of cytoplasm and vacuoles viscoelasticity in adapted cells has also a relative dimension. This work provides the first semi-quantitative data, to our knowledge, for viscoelastic cellular changes in plant cells adapted to growth under salt and osmotic stress conditions and exposed to subsequent short-term stress. Applied methods provide evidence that adaptation to hyperosmotic stress leads to different ratio in the protoplast and environment qualities that helps to maintain cell integrity. Many challenges remain in the field of plant mechanobiology, to which our approach could provide valuable insight into plant cell responses to the environment and the further development of useful methods.

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Section: Discussionmentioning

confidence: 99%

The viscoelastic properties ofNicotiana tabacumBY-2 suspension cell lines adapted to high osmolarity

Skrzypczak,

Pochylski,

Rapp

et al. 2024

Preprint

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“…The protective effect of STRP is very likely ascribed to its biochemical properties. In fact, IDPs are typically involved in seed germination and in the regulation of early stages of plant development by sensing unfavorable environmental conditions and consequently protecting macromolecules and intracellular structures [31,40].…”

Section: Discussionmentioning

confidence: 99%

The Salt Tolerance–Related Protein (STRP) Is a Positive Regulator of the Response to Salt Stress in Arabidopsis thaliana

Fiorillo

Manai

Visconti

et al. 2023

Plants

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Salt stress is a major abiotic stress limiting plant survival and crop productivity. Plant adaptation to salt stress involves complex responses, including changes in gene expression, regulation of hormone signaling, and production of stress-responsive proteins. The Salt Tolerance–Related Protein (STRP) has been recently characterized as a Late Embryogenesis Abundant (LEA)–like, intrinsically disordered protein involved in plant responses to cold stress. In addition, STRP has been proposed as a mediator of salt stress response in Arabidopsis thaliana, but its role has still to be fully clarified. Here, we investigated the role of STRP in salt stress responses in A. thaliana. The protein rapidly accumulates under salt stress due to a reduction of proteasome–mediated degradation. Physiological and biochemical responses of the strp mutant and STRP–overexpressing (STRP OE) plants demonstrate that salt stress impairs seed germination and seedling development more markedly in the strp mutant than in A. thaliana wild type (wt). At the same time, the inhibitory effect is significantly reduced in STRP OE plants. Moreover, the strp mutant has a lower ability to counteract oxidative stress, cannot accumulate the osmocompatible solute proline, and does not increase abscisic acid (ABA) levels in response to salinity stress. Accordingly, the opposite effect was observed in STRP OE plants. Overall, obtained results suggest that STRP performs its protective functions by reducing the oxidative burst induced by salt stress, and plays a role in the osmotic adjustment mechanisms required to preserve cellular homeostasis. These findings propose STRP as a critical component of the response mechanisms to saline stress in A. thaliana.

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“…The binding plasticity of disorder regions assists in the attachment to other gene families like NAC, GRAS, and dehydrins. These interactions generate a signaling cascade in developmental processes like seed development, response to hormonal changes, and drought stress ( Hsiao, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, comprehensive characterization of transcription factors, cis-regulatory elements, protein homology, and protein interaction network of DREB gene family in Solanum lycopersicum

et al. 2022

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Tomato is a drought-sensitive crop which has high susceptibility to adverse climatic changes. Dehydration-responsive element-binding (DREB) are significant plant transcription factors that have a vital role in regulating plant abiotic stress tolerance by networking with DRE/CRT cis-regulatory elements in response to stresses. In this study, bioinformatics analysis was performed to conduct the genome-wide identification and characterization of DREB genes and promoter elements in Solanum lycopersicum. In genome-wide coverage, 58 SlDREB genes were discovered on 12 chromosomes that justified the criteria of the presence of AP2 domain as conserved motifs. Intron–exon organization and motif analysis showed consistency with phylogenetic analysis and confirmed the absence of the A3 class, thus dividing the SlDREB genes into five categories. Gene expansion was observed through tandem duplication and segmental duplication gene events in SlDREB genes. Ka/Ks values were calculated in ortholog pairs that indicated divergence time and occurrence of purification selection during the evolutionary period. Synteny analysis demonstrated that 32 out of 58 and 47 out of 58 SlDREB genes were orthologs to Arabidopsis and Solanum tuberosum, respectively. Subcellular localization predicted that SlDREB genes were present in the nucleus and performed primary functions in DNA binding to regulate the transcriptional processes according to gene ontology. Cis-acting regulatory element analysis revealed the presence of 103 motifs in 2.5-kbp upstream promoter sequences of 58 SlDREB genes. Five representative SlDREB proteins were selected from the resultant DREB subgroups for 3D protein modeling through the Phyre2 server. All models confirmed about 90% residues in the favorable region through Ramachandran plot analysis. Moreover, active catalytic sites and occurrence in disorder regions indicated the structural and functional flexibility of SlDREB proteins. Protein association networks through STRING software suggested the potential interactors that belong to different gene families and are involved in regulating similar functional and biological processes. Transcriptome data analysis has revealed that the SlDREB gene family is engaged in defense response against drought and heat stress conditions in tomato. Overall, this comprehensive research reveals the identification and characterization of SlDREB genes that provide potential knowledge for improving abiotic stress tolerance in tomato.

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Plant Protein Disorder: Spatial Regulation, Broad Specificity, Switch of Signaling and Physiological Status

Cited by 8 publications

References 69 publications

The viscoelastic properties ofNicotiana tabacumBY-2 suspension cell lines adapted to high osmolarity

The viscoelastic properties ofNicotiana tabacumBY-2 suspension cell lines adapted to high osmolarity

The Salt Tolerance–Related Protein (STRP) Is a Positive Regulator of the Response to Salt Stress in Arabidopsis thaliana

Genome-wide identification, comprehensive characterization of transcription factors, cis-regulatory elements, protein homology, and protein interaction network of DREB gene family in Solanum lycopersicum

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