Relevance. The use of microbiological preparations in agriculture is one of the areas of biologization, based on the modern achievement of science. Complex microfertilizers, growth stimulants, humic and bacterial preparations contribute to the activation of production processes of spring barley, especially at earlier phases of ontogenesis: sowing and yielding qualities of seeds, field germination and preservation of plants, rates of accumulation of vegetative mass, density of productive stem stand increase. Methods. The testing of the drugs was carried out in 2022–2023 at the experimental field of the Institute of Seed Production and Agricultural Technologies, a branch of the Federal State Budgetary Scientific Institution “Federal Scientific Agroengineering Center All-Russian Institute of Mechanization”. The objects of the research were spring barley of the varieties Raphael, Znatny, Yaromir, microbiological preparations “Organit N, Zh” and “Organit R, Zh”. The soil of the site is dark-gray forest heavy loamy, humus content is 3.9%. The predecessor is winter wheat. Agricultural techniques of experience generally accepted for the cultivation of crops in the Ryazan region. During the growing season, observations were made on the phases of crop development. The studies were carried out in accordance with the “Field Experiment Methodology”, the Methodological Guidelines for Registration Tests of New Forms of Fertilizers, Biological Preparations and Plant Growth Regulators, and the economic efficiency in accordance with the Accounting Guidelines. Results. It was found that spring barley varieties reacted differently to the studied preparations. The survival rate of plants for harvesting is from 83.5 to 94.3%. Increase in yield up to 19% at Znatny and up to 18.7% at Rafael. The Yaromir variety showed stable productivity in all variants of the experiment, an increase of up to 3.9%, with a control yield of 6.16 t/ha.