Plant Products in the Treatment and Control of Filariasis and Other Helminth Infections and Assay Systems for Antifilarial/Anthelmintic Activity

Murthy, P. K.; Joseph, Sujith; Murthy, P. S. R.

doi:10.1055/s-0030-1250452

Cited by 41 publications

(28 citation statements)

References 101 publications

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“…Phloroglucinols, such as aspidin, deaspidin, and filixic acid, which can be found in Dryopteris filixmas (Dryopteridaceae) can paralyze helminthes, and this effect is more pronounced on cestodes (Murthy et al, 2011;Wink, 2012).…”

Section: Effects On Mobilitymentioning

confidence: 99%

Botanicals: a natural approach to control ascaridiosis in poultry

Symeonidou

Bonos

Moustakidis

et al. 2018

J Hellenic Vet Med Soc

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Parasites (protozoa, helminthes, arthropods) represent a main threat for poultry worldwide. Among helminthes, nematodes constitute the most important group of parasites of poultry. The nematode Ascaridia galli, the cause of ascaridiosis in poultry, is one of the most important and prevalent parasites, resulting in serious economic losses, associated with the treatment cost, the decreased feed efficiency, and the poor egg and meat production. During the past few decades the indiscriminate use of anthelmintic drugs has generated several cases of resistance in helminthes in poultry, situation which is coupled with the severity of residues in poultry products. For this reason, nowadays attention has been drawn to the use of botanicals in poultry diet, due to their anthelmintic properties. Furthermore, the dietary use eco-friend ly of these plant derived substances compared to conventional synthetic anthelmintic drugs is considered as a natural and ecofriendly approach by the consumers. The focus of the present review is to recapitulate the studies, both in vivo and in vitro, that have demonstrated the anthelmintic efficacy of various dietary botanicals in controlling poultry ascaridiosis.

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Section: Effects On Mobilitymentioning

confidence: 99%

Botanicals: a natural approach to control ascaridiosis in poultry

Symeonidou

Bonos

Moustakidis

et al. 2018

J Hellenic Vet Med Soc

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“…Filariasis is a chronic infection of considerable morbidity in tropical and subtropical countries (Katiyar and Singh 2011;Murthy et al 2011). Setaria cervi is an important filarid inhabiting the peritoneal cavity of buffaloes, causing peritonitis, intestinal occlusion and cerebro-setariasis in rare instances (Sarwar 1945;Innes and Shoho 1953;Pachauri 1972;Tung et al 2003) and neurological disorders in human by Setaria sp.…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of antifilarial effect of Azadirachta indica leaves extract in different solvents on the microfilariae of Setaria cervi

Kausar

2016

J Parasit Dis

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Polar solvent extracts of Azadirachta indica leaves were investigated to evaluate in vitro anthelmintic activity on the microfilariae of Setaria cervi. Four extracts of A. indica leaves were prepared with different solvents in increasing order of polarity (diethyl ether \ chloroform \ ethanol \ methanol). Stock solution of the extract of A. indica leaves was prepared in Ringer's solution at the concentrations of 50, 100 and 200 lg/ml and used against microfilariae in three replicates. Ringer's solution was used as experimental control. Mortality rates of microfilariae were recorded for each concentration after 45, 90 and 135 min, respectively. All the solvent extracts show significant anthelmintic activity at the concentration of 200 lg/ml after 135 min of incubation. Highest mortality rate of microfilariae was observed in methanol and ethanol extracts throughout the incubation period compared to chloroform and diethyl ether extracts which showed low mortality rates. Dose dependent effect of ethanol and chloroform showed highest and lowest ranking when analyzed by regression values and correlation of regression. Solvents extracts of A. indica leaves showed anthelmintic effect and therefore may be used as phytopharmacological agents in future.

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“…Since, DEC and IVM both are microfilaricides with poor or no activity on adult parasites [9], the peripheral blood microfilaremia reappears in patients after a certain period of withdrawal of the drug. This depressing perspective demands, an urgent need for new molecular structures associated with macrofilaricidal activity/or sterilizing the adult worms is therefore needed [8]–[10] as adult parasites not only produce millions of microfilariae (mf) that are picked up by the mosquito vector and transmitted, but are also responsible for the debilitating pathological lesions. Therefore, macrofilaricidal agents are the need of hour, which not only adversely affect the target but should have also very low or no side effect [11].…”

Section: Introductionmentioning

confidence: 99%

In Vitro, In Silico and In Vivo Studies of Ursolic Acid as an Anti-Filarial Agent

et al. 2014

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As part of our drug discovery program for anti-filarial agents from Indian medicinal plants, leaves of Eucalyptus tereticornis were chemically investigated, which resulted in the isolation and characterization of an anti-filarial agent, ursolic acid (UA) as a major constituent. Antifilarial activity of UA against the human lymphatic filarial parasite Brugia malayi using in vitro and in vivo assays, and in silico docking search on glutathione-s-transferase (GST) parasitic enzyme were carried out. The UA was lethal to microfilariae (mf; LC100: 50; IC50: 8.84 µM) and female adult worms (LC100: 100; IC50: 35.36 µM) as observed by motility assay; it exerted 86% inhibition in MTT reduction potential of the adult parasites. The selectivity index (SI) of UA for the parasites was found safe. This was supported by the molecular docking studies, which showed adequate docking (LibDock) scores for UA (−8.6) with respect to the standard antifilarial drugs, ivermectin (IVM −8.4) and diethylcarbamazine (DEC-C −4.6) on glutathione-s-transferase enzyme. Further, in silico pharmacokinetic and drug-likeness studies showed that UA possesses drug-like properties. Furthermore, UA was evaluated in vivo in B. malayi-M. coucha model (natural infection), which showed 54% macrofilaricidal activity, 56% female worm sterility and almost unchanged microfilaraemia maintained throughout observation period with no adverse effect on the host. Thus, in conclusion in vitro, in silico and in vivo results indicate that UA is a promising, inexpensive, widely available natural lead, which can be designed and developed into a macrofilaricidal drug. To the best of our knowledge this is the first ever report on the anti-filarial potential of UA from E. tereticornis, which is in full agreement with the Thomson Reuter's ‘Metadrug’ tool screening predictions.

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Plant Products in the Treatment and Control of Filariasis and Other Helminth Infections and Assay Systems for Antifilarial/Anthelmintic Activity

Cited by 41 publications

References 101 publications

Botanicals: a natural approach to control ascaridiosis in poultry

Botanicals: a natural approach to control ascaridiosis in poultry

In vitro evaluation of antifilarial effect of Azadirachta indica leaves extract in different solvents on the microfilariae of Setaria cervi

In Vitro, In Silico and In Vivo Studies of Ursolic Acid as an Anti-Filarial Agent

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