Plant Production of Veterinary Vaccines and Therapeutics

Hammond, Rosemarie W.; Nemchinov, Lev G.

doi:10.1007/978-3-540-70868-1_5

Cited by 10 publications

(4 citation statements)

References 93 publications

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“…Plant-based oral vaccines are another delivery system that has been tested in recent years [58] – [61] . Seed crops such as rice, maize, and soybean appear to be suitable expression and delivery systems that offer several advantages, such as resistance to intestinal enzymes, rapid scale-up of exogenous antigens, low-cost production, and a decreased risk of contamination by human pathogens [62] , [63] .…”

Section: Oral Vaccine Delivery Systemsmentioning

confidence: 99%

Enhancing Oral Vaccine Potency by Targeting Intestinal M Cells

et al. 2010

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The immune system in the gastrointestinal tract plays a crucial role in the control of infection, as it constitutes the first line of defense against mucosal pathogens. The attractive features of oral immunization have led to the exploration of a variety of oral delivery systems. However, none of these oral delivery systems have been applied to existing commercial vaccines. To overcome this, a new generation of oral vaccine delivery systems that target antigens to gut-associated lymphoid tissue is required. One promising approach is to exploit the potential of microfold (M) cells by mimicking the entry of pathogens into these cells. Targeting specific receptors on the apical surface of M cells might enhance the entry of antigens, initiating the immune response and consequently leading to protection against mucosal pathogens. In this article, we briefly review the challenges associated with current oral vaccine delivery systems and discuss strategies that might potentially target mouse and human intestinal M cells.

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Section: Oral Vaccine Delivery Systemsmentioning

confidence: 99%

Enhancing Oral Vaccine Potency by Targeting Intestinal M Cells

et al. 2010

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“…Some differences in glycosylation patterns of proteins produced in plant and mammalian cells apparently caused no significant effect on their antigen characteristic or specificity [10] and may be overcome by expression in host of human glycosyltransferases [11]. Numerous examples of immune responses in humans or animals caused by oral delivery of plant-based vaccines were reviewed in recent publications [12][13][14]. Up to now no clear evidence were reported about possible pathogenic action of plant viruses on humans, that makes the recombinant proteins of plant origin safer.…”

Section: During the Last Decade Interferons Are Regarded As Potent Camentioning

confidence: 99%

Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants

et al. 2012

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“…Transmissible gastroenteritis virus (TGEV) is a coronavirus that significantly affects swine production (Hammond and Nemchinov 2009). The disease is highly infectious, and the severe diarrhea and vomiting produce high mortality in young piglets.…”

Section: Transmissible Gastroenteritis Virusmentioning

confidence: 99%

Plant-Derived Antigens as Mucosal Vaccines

Mason

Herbst-Kralovetz

2011

Current Topics in Microbiology and Immunology

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During the last two decades, researchers have developed robust systems for recombinant subunit vaccine production in plants. Stably and transiently transformed plants have particular advantages that enable immunization of humans and animals via mucosal delivery. The initial goal to immunize orally by ingestion of plant-derived antigens has proven difficult to attain, although many studies have demonstrated antibody production in both humans and animals, and in a few cases, protection against pathogen challenge. Substantial hurdles for this strategy are low-antigen content in crudely processed plant material and limited antigen stability in the gut. An alternative is intranasal delivery of purified plant-derived antigens expressed with robust viral vectors, especially virus-like particles. The use of pattern recognition receptor agonists as adjuvants for mucosal delivery of plant-derived antigens can substantially enhance serum and mucosal antibody responses. In this chapter, we briefly review the methods for recombinant protein expression in plants, and describe progress with human and animal vaccines that use mucosal delivery routes. We do not attempt to compile a comprehensive list, but focus on studies that progressed to clinical trials or those that showed strong indications of efficacy in animals. Finally, we discuss some regulatory concerns regarding plant-based vaccines.

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Plant Production of Veterinary Vaccines and Therapeutics

Cited by 10 publications

References 93 publications

Enhancing Oral Vaccine Potency by Targeting Intestinal M Cells

Enhancing Oral Vaccine Potency by Targeting Intestinal M Cells

Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants

Plant-Derived Antigens as Mucosal Vaccines

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