Plant-produced Bluetongue chimaeric VLP vaccine candidates elicit serotype-specific immunity in sheep

Mokoena, Nobalanda B.; Moetlhoa, Boitumelo; Rutkowska, Daria; Mamputha, Sipho; Dibakwane, Vusi S.; Tsekoa, Tsepo L.; O’Kennedy, Martha M.

doi:10.1016/j.vaccine.2019.08.042

Cited by 16 publications

(14 citation statements)

References 20 publications

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“…Leaves can be dried prior to protein extraction to reduce the volume of buffers required [ 65 ]. Ultrafiltration can be used for concentration, but direct use of filtered plant extract has been reported for VLP vaccines, which would provide a cost-effective method for vaccine preparation [ 66 , 67 ].…”

Section: Pipeline For Production Of Plant-based Vaccinesmentioning

confidence: 99%

“…Crude leaf extracts were used to immunise mice; extracts containing HA oligomers were found to induce higher titres of neutralising antibody than extracts containing HA trimers [ 75 ]. Similarly, more chickens survived a lethal challenge after immunisation with crude leaf extracts containing HA oligomers than with trimers [ 67 ].…”

Section: Pipeline For Production Of Plant-based Vaccinesmentioning

confidence: 99%

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Development of Plant-Based Vaccines for Prevention of Avian Influenza and Newcastle Disease in Poultry

et al. 2022

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Viral diseases, including avian influenza (AI) and Newcastle disease (ND), are an important cause of morbidity and mortality in poultry, resulting in significant economic losses. Despite the availability of commercial vaccines for the major viral diseases of poultry, these diseases continue to pose a significant risk to global food security. There are multiple factors for this: vaccine costs may be prohibitive, cold chain storage for attenuated live-virus vaccines may not be achievable, and commercial vaccines may protect poorly against local emerging strains. The development of transient gene expression systems in plants provides a versatile and robust tool to generate a high yield of recombinant proteins with superior speed while managing to achieve cost-efficient production. Plant-derived vaccines offer good stability and safety these include both subunit and virus-like particle (VLP) vaccines. VLPs offer potential benefits compared to currently available traditional vaccines, including significant reductions in virus shedding and the ability to differentiate between infected and vaccinated birds (DIVA). This review discusses the current state of plant-based vaccines for prevention of the AI and ND in poultry, challenges in their development, and potential for expanding their use in low- and middle-income countries.

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Section: Pipeline For Production Of Plant-based Vaccinesmentioning

confidence: 99%

Section: Pipeline For Production Of Plant-based Vaccinesmentioning

confidence: 99%

Development of Plant-Based Vaccines for Prevention of Avian Influenza and Newcastle Disease in Poultry

et al. 2022

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“…Importantly, VP3 and VP7 are relatively well conserved between serotypes so that outer capsid proteins from different serotypes can be coated onto heterologous inner core proteins, which implies an advantage of this VLP system to generate vaccines to new serotypes by replacing VP2, the most variable BTV protein [ 163 ]. Apart from a baculovirus expression system, a plant-based high-level expression system was also implemented to produce assembled subcore-, core- and virus-like particles of BTV [ 164 , 165 ].…”

Section: Bluetongue Virus (Btv)mentioning

confidence: 99%

Nanoparticle- and Microparticle-Based Vaccines against Orbiviruses of Veterinary Importance

et al. 2022

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Bluetongue virus (BTV) and African horse sickness virus (AHSV) are widespread arboviruses that cause important economic losses in the livestock and equine industries, respectively. In addition to these, another arthropod-transmitted orbivirus known as epizootic hemorrhagic disease virus (EHDV) entails a major threat as there is a conducive landscape that nurtures its emergence in non-endemic countries. To date, only vaccinations with live attenuated or inactivated vaccines permit the control of these three viral diseases, although important drawbacks, e.g., low safety profile and effectiveness, and lack of DIVA (differentiation of infected from vaccinated animals) properties, constrain their usage as prophylactic measures. Moreover, a substantial number of serotypes of BTV, AHSV and EHDV have been described, with poor induction of cross-protective immune responses among serotypes. In the context of next-generation vaccine development, antigen delivery systems based on nano- or microparticles have gathered significant attention during the last few decades. A diversity of technologies, such as virus-like particles or self-assembled protein complexes, have been implemented for vaccine design against these viruses. In this work, we offer a comprehensive review of the nano- and microparticulated vaccine candidates against these three relevant orbiviruses. Additionally, we also review an innovative technology for antigen delivery based on the avian reovirus nonstructural protein muNS and we explore the prospective functionality of the nonstructural protein NS1 nanotubules as a BTV-based delivery platform.

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“…Bluetongue (BT) is a non-contagious, infectious, World Organisation for Animal Health (OIE) notifiable disease of ruminants caused by the Bluetongue virus (BTV, family Reoviridae, genus Orbivirus) and is spread by Culicoides spp. biting midges (Coetzee, Stokstad, Venter, Myrmel, & Van Vuuren, 2012;Kar, Ghosh, & Roy, 2004;Mokoena et al, 2019;Schulz et al, 2016). There are 27 distinct known BTV serotypes determined by the outer viral-capsid protein VP2 encoded by segment 2 of the dsRNA genome (Coetzee et al, 2012;Mokoena et al, 2019;Schulz et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…biting midges (Coetzee, Stokstad, Venter, Myrmel, & Van Vuuren, 2012;Kar, Ghosh, & Roy, 2004;Mokoena et al, 2019;Schulz et al, 2016). There are 27 distinct known BTV serotypes determined by the outer viral-capsid protein VP2 encoded by segment 2 of the dsRNA genome (Coetzee et al, 2012;Mokoena et al, 2019;Schulz et al, 2016). Furthermore, BTV infected sheep often show severe clinical signs, while cattle, goats and camelids are usually asymptomatic, although some clinical cases in cattle have been observed during the North European outbreak of BTV-8 (Backx, Heutink, van Rooij, & van Rijn, 2007;Caporale et al, 2014;Dal Pozzo, De Clercq, et al, 2009;Dal Pozzo, Saegerman, & Thiry, 2009;Eschbaumer et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Soluble Expression, Purification and Characterization of the VP3 of the 1st Serotype Bluetongue Virus

Wang

Han

Jia

et al. 2020

Preprint

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Bluetongue (BT) is a non-contact infectious disease of domestic or wild ruminants caused by the Bluetongue virus (BTV). It is transmitted by Culicoides biting midges. BTV mainly infect sheep, and animals infected with BTV usually show clinical symptoms such as fever, mucosal edema, and ulcers. BTV virus is an icosahedral symmetric RNA virus with 27 different serotypes. BTV consists of 7 structural proteins (VP1-VP7) and 4 non-structural proteins (NS1, NS2, NS3/NS3a, NS4, NS5). The VP3 encoded by the L3 gene is relatively high conserved structural protein, and constitute the inner symmetric icosahedral core shell of virus particle. In this study, VP3 recombinant protein of bluetongue I virus was successfully expressed and purified by prokaryotic expression system. Moreover, to increase the amount of soluble protein, we used chaperone protein ptf16 and two fusion proteins NusA and TRX. The results show that chaperone protein can increase the solubility of VP3. Then the expression conditions were optimized and VP3 was purified by nickel affinity chromatography. The VP3 immunize Balb/c mice and the results show that the serum titer is 1:1.28×105. In the Dot-ELISA assay, we found recombinant protein VP3 react with the serum from immunized mice by inactivate BTV-1. The results of IFA showed that the antibody produced by immunized mice with recombinant protein VP3 could react with the VP3 protein expressed in 293T cell. In conclusion, we expressed the recombinant protein VP3 with the same conformation as eukaryotic expression system and had same immunogenicity with inactivated virus, which laid a foundation for further study on the structure and function of BTV.

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Plant-produced Bluetongue chimaeric VLP vaccine candidates elicit serotype-specific immunity in sheep

Cited by 16 publications

References 20 publications

Development of Plant-Based Vaccines for Prevention of Avian Influenza and Newcastle Disease in Poultry

Development of Plant-Based Vaccines for Prevention of Avian Influenza and Newcastle Disease in Poultry

Nanoparticle- and Microparticle-Based Vaccines against Orbiviruses of Veterinary Importance

Soluble Expression, Purification and Characterization of the VP3 of the 1st Serotype Bluetongue Virus

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