Plant Polygalacturonases Involved in Cell Elongation and Separation—The Same but Different?

Babu, Yashodar; Bayer, Martin

doi:10.3390/plants3040613

Cited by 34 publications

(22 citation statements)

References 55 publications

(80 reference statements)

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“…Polygalacturonase (PG) activity got lowered in presence of feldspar which directly proportionate to reduced pathogenic effect of RS (Gawade et al, 2017). In our study, lowering of PG in the RS treatments after the addition of Fe also corresponds to reduced loosening of cell wall which otherwise occurs due to breakdown of homogalacturonan by PG (Babu and Bayer, 2014). Pectate lyase (PL) reported for the breakdown of polysaccharides in plant cell wall thereby inducing defense (Vorwerk et al, 2004).…”

Section: Discussionsupporting

confidence: 51%

Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress

et al. 2020

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Silicon (Si), the second most abundant element on earth, remains unavailable for plants' uptake due to its poor solubility. Microbial interventions to convert it in soluble forms are well documented. However, studies on discrimination of Si and P solubilizing microbes due to common estimation method and sharing of solubilization mechanism are still obscure. A defined differential media, i.e. silicon-solubilizing media (NBRISSM) is developed to screen Si solubilizers. NBRISN13 (Bacillus amyloliquefaciens), a Si solubilizer, exhibiting antagonistic property against Rhizoctonia solani, was further validated for disease resistance. The key finding of the work is that NBRISSM is a novel differential media for screening Si solubilizers, distinct from P solubilizers. Dominance of Pseudomonas and Bacillus spp. for the function of Si solubilization was observed during diversity analysis of Si solubilizers isolated from different rhizospheres. Sphingobacterium sp., a different strain has been identified for silicon solubilization other than Pseudomonas and Bacillus sp. Role of acidic phosphatase during Si solubilization has been firstly reported in our study in addition to other pH dependent phenomenon. Study also showed the combinatorial effect of feldspar and NBRISN13 on elicited immune response through (i) increased Si uptake, (ii) reduced disease severity, (iii) modulation of cell wall degrading and antioxidative enzyme activities, and (iv) induced defense responsive gene expression.

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Section: Discussionsupporting

confidence: 51%

Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress

et al. 2020

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“…Polygalacturonase is a major component of the pectin remodeling and disassembly network [47]. It is involved in catalyzing the degradation of pectin in the cell wall via the hydrolytic cleavage of glyosidic bonds [48], probably making seeds soften and shorten the cooking time.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study of Multi-Traits in Common Bean (Phaseolus Vulgaris L.)

Binagwa¹,

Bonsi²,

He³

et al. 2020

Preprint

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Background: Micronutrient deficiencies caused by lack of Iron (Fe), Zinc (Zn) and Vitamin A have negative effects on human health worldwide. Even though common bean meets Fe and Zn deficiencies based on recommended dietary allowances, the cooking time remains an important challenge. Understanding the genome organization, the loci and genes localization controlling multiple traits is crucial for developing enhanced micronutrient content and improved common bean with short cooking time. Results: In this study, GWAS method was used to determine SNPs associated with four traits studied, including water uptake, cooking time, Fe and Zn concentration level, using ~5,000 SNPs for a panel of 206 genotypes. By the analysis of population structure, 206 common bean genotypes were formed in three groups, the Mesoamerican gene pool, the Andean gene pool, and the admixture gene pool using the Bayesian method. With the significance levels between p = 2.94 x 10-08 to p = 8.33 x 10-03 and phenotypic variation of 8.26% to 18.19%, total of 10 SNPs was found to be significantly associated with traits on chromosomes Pv03, Pv04 and Pv10. Among of these SNPs, four SNPs were associated with water uptake, another four SNPs with the cooking time, one SNP with Zn concentration and one of less significant SNP associated with Fe concentration. Within the range or nearby the peak of significant SNP, putative loci encoding pectin degradation, cell structure and cellular exchange/transport were identified, suggesting these loci may play important roles in modulating expression of traits.Conclusion: The resultant data in this study contributed on deciphering the molecular mechanisms associated with water uptake, cooking time and micronutrient contents in common bean. The investigation of association between phenotypic and genotypic data has showed that GWAS approach is a powerful tool to dissect genetic architecture of complex traits and cooperate molecular breeding for advanced genotypes with enhanced micronutrients and short cooking time.

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“…Polygalacturonases are another group of genes coding for enzymes involved in degradation of the cell wall, specifically homogalacturonan type of pectin. These enzymes function in a wide range of developmental processes, including cell separation and cell elongation to control the shape of the plant architecture (Gonzalez-Carranza et al, 2007;Babu and Bayer, 2014). They play important roles in the primary cell wall of meristematic and elongating cells, before secondary cell walls can fortify the fibers (Zhong and Ye, 2007).…”

Section: Genetic Architecture Underlying Hemp Fiber Qualitymentioning

confidence: 99%

“…They play important roles in the primary cell wall of meristematic and elongating cells, before secondary cell walls can fortify the fibers (Zhong and Ye, 2007). In Arabidopsis, 69 polygalacturonases has been identified and the alteration of several of those was shown to produce aberrations in plant developmental processes [reviewed in Babu and Bayer (2014)]. For instance, polygalacturonase involved in expansion1 (pgx1) was shown to be involved in hypocotyl elongation.…”

Section: Genetic Architecture Underlying Hemp Fiber Qualitymentioning

confidence: 99%

Elucidating the Genetic Architecture of Fiber Quality in Hemp (Cannabis sativa L.) Using a Genome-Wide Association Study

et al. 2020

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Hemp (Cannabis sativa L.) is a bast-fiber crop with a great potential in the emerging bio-based economy. Yet, hemp breeding for fiber quality is restricted and that is mainly due to the limited knowledge of the genetic architecture of its fiber quality. A panel of 123 hemp accessions, with large phenotypic variability, was used to study the genetic basis of seven cell wall and bast fiber traits relevant to fiber quality. These traits showed large genetic variance components and high values of broad sense heritability in this hemp panel, as concluded from the phenotypic evaluation across three test locations with contrasting environments. The hemp panel was genotyped using restriction site associated DNA sequencing (RAD-seq). Subsequently, a large set (> 600,000) of selected genome-wide single nucleotide polymorphism (SNP) markers was used for a genome-wide association study (GWAS) approach to get insights into quantitative trait loci (QTLs) controlling fiber quality traits. In absence of a complete hemp genome sequence, identification of QTLs was based on the following characteristics: (i) association level to traits, (ii) fraction of explained trait variance, (iii) collinearity between QTLs, and (iv) detection across different environments. Using this approach, 16 QTLs were identified across locations for different fiber quality traits, including contents of glucose, glucuronic acid, mannose, xylose, lignin, and bast fiber content. Among them, six were found across the three environments. The genetic markers composing the QTLs that are common across locations are valuable tools to develop novel genotypes of hemp with improved fiber quality. Underneath the QTLs, 12 candidate genes were identified which are likely to be involved in the biosynthesis and modification of monosaccharides, polysaccharides, and lignin. These candidate genes were suggested to play an important role in determining fiber quality in hemp. This study provides new insights into the genetic architecture of fiber traits, identifies QTLs and candidate genes that form the basis for molecular breeding for high fiber quality hemp cultivars.

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Plant Polygalacturonases Involved in Cell Elongation and Separation—The Same but Different?

Cited by 34 publications

References 55 publications

Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress

Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress

Genome-Wide Association Study of Multi-Traits in Common Bean (Phaseolus Vulgaris L.)

Elucidating the Genetic Architecture of Fiber Quality in Hemp (Cannabis sativa L.) Using a Genome-Wide Association Study

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