Plant Pleiotropic Drug Resistance Transporters: Transport Mechanism, Gene Expression, and Function

Nuruzzaman, Mohammed; Zhang, Ru; Cao, Hongzhe; Luo, Zhitian

doi:10.1111/jipb.12196

Cited by 60 publications

(36 citation statements)

References 108 publications

(170 reference statements)

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“…Regarding other heavy metals, some plant full-size ABCG (PDR) transporters confer cadmium and lead tolerance (38)(39)(40). Therefore, this work presents the first description of the role of an ABCG transporter in the resistance of Leishmania to Sb III and As III .…”

Section: Resultsmentioning

confidence: 99%

The LABCG2 Transporter from the Protozoan Parasite Leishmania Is Involved in Antimony Resistance

Perea

Manzano

Castanys

et al. 2016

Antimicrob Agents Chemother

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Treatment for leishmaniasis, which is caused by Leishmania protozoan parasites, currently relies on a reduced arsenal of drugs. However, the significant increase in the incidence of drug therapeutic failure and the growing resistance to first-line drugs like antimonials in some areas of Northern India and Nepal limit the control of this parasitic disease. Understanding the molecular mechanisms of resistance in Leishmania is now a matter of urgency to optimize drugs used and to identify novel drug targets to block or reverse resistant mechanisms. Some members of the family of ATP-binding cassette (ABC) transporters in Leishmania have been associated with drug resistance. In this study, we have focused our interest to characterize LABCG2's involvement in drug resistance in Leishmania. Leishmania major parasites overexpressing the ABC protein transporter LABCG2 were generated in order to assess how LABCG2 is involved in drug resistance. Assays of susceptibility to different leishmanicidal agents were carried out. Analysis of the drug resistance profile revealed that Leishmania parasites overexpressing LABCG2 were resistant to antimony, as they demonstrated a reduced accumulation of Sb III due to an increase in drug efflux. Additionally, LABCG2 was able to transport thiols in the presence of Sb III . Biotinylation assays using parasites expressing LABCG2 fused with an N-terminal green fluorescent protein tag revealed that LABCG2 is partially localized in the plasma membrane; this supports data from previous studies which suggested that LABCG2 is localized in intracellular vesicles that fuse with the plasma membrane during exocytosis. In conclusion, Leishmania LABCG2 probably confers antimony resistance by sequestering metal-thiol conjugates within vesicles and through further exocytosis by means of the parasite's flagellar pocket.

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Section: Resultsmentioning

confidence: 99%

The LABCG2 Transporter from the Protozoan Parasite Leishmania Is Involved in Antimony Resistance

Perea

Manzano

Castanys

et al. 2016

Antimicrob Agents Chemother

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“…OsPDR9 ( Os01g0609300 ) encodes one of the 23 pleiotropic drug resistance (PDR)-like ATP-binding cassette (ABC) transporters in rice [93]. Plant PDR-like ABC transporters are expressed in response to various biotic and abiotic stresses and transport a diverse array of molecules across membranes [94]. OsPDR9 was reported to be induced by jasmonic acid, heavy metals, high salt, hypoxic stress and redox perturbations, suggesting its role in stress responses [95] (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Responses to Imbalanced Carbon: Nitrogen Availabilities in Rice Seedlings

et al. 2016

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The internal C:N balance must be tightly controlled for the normal growth and development of plants. However, the underlying mechanisms, by which plants sense and balance the intracellular C:N status correspondingly to exogenous C:N availabilities remain elusive. In this study, we use comparative gene expression analysis to identify genes that are responsive to imbalanced C:N treatments in the aerial parts of rice seedlings. Transcripts of rice seedlings treated with four C:N availabilities (1:1, 1:60, 60:1 and 60:60) were compared and two groups of genes were classified: high C:low N responsive genes and low C:high N responsive genes. Our analysis identified several functional correlated genes including chalcone synthase (CHS), chlorophyll a-b binding protein (CAB) and other genes that are implicated in C:N balancing mechanism, such as alternative oxidase 1B (OsAOX1B), malate dehydrogenase (OsMDH) and lysine and histidine specific transporter 1 (OsLHT1). Additionally, six jasmonate synthetic genes and key regulatory genes involved in abiotic and biotic stresses, such as OsMYB4, autoinhibited calcium ATPase 3 (OsACA3) and pleiotropic drug resistance 9 (OsPDR9), were differentially expressed under high C:low N treatment. Gene ontology analysis showed that high C:low N up-regulated genes were primarily enriched in fatty acid biosynthesis and defense responses. Coexpression network analysis of these genes identified eight jasmonate ZIM domain protein (OsJAZ) genes and several defense response related regulators, suggesting that high C:low N status may act as a stress condition, which induces defense responses mediated by jasmonate signaling pathway. Our transcriptome analysis shed new light on the C:N balancing mechanisms and revealed several important regulators of C:N status in rice seedlings.

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“…In addition to MRP, other ABC transporters have been shown to be involved in the transport of plant secondary metabolites, such as PDR transporter (Schulz and Kolukisaglu 2006;Crouzet et al 2013;Nuruzzaman et al 2014). In fact, an ABC transporter of Arabidopsis PDR12 (AtPDR12)/ ABCG40 has been reported to be a plasma-membrane ABAuptake transporter in guard cells and other types of plant cells.…”

Section: Discussionmentioning

confidence: 98%

“…Based on structural features, these ABC transporters have been divided into several subfamilies including full size and half size transporters. However, three ABC proteins are known to be responsible for chemoresistance: pleiotropic drug resistance (PDR, or ABCG), multidrug resistance (P-glycoprotein/MDR, or ABCB), and multidrug resistance-associated protein (MRP, or ABCC) (Litman et al 2001;Nuruzzaman et al 2014).…”

Section: Introductionmentioning

confidence: 99%

MRP functional activity and character in the dinoflagellate Prorocentrum lima

et al. 2015

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Using laser scanning confocal microscope (LACM) and flow cytometry (FCM), we found that MK571, a specific inhibitor of multidrug resistance-associated protein (MRP), could increase the accumulation of calcein-AM in Prorocentrum lima, a DSP toxin-producing dinoflagellate. This led to the assumption that the cells of P. lima possess MRP transporters in the plasma membrane. The contents of okadaic acid (OA), a main component of DSP toxins, and MRP activities in P. lima were determined at 4, 20, 28, and 42 days after culture and at 38 day under N-and P-limited conditions. We found that both the OA level and MRP activity in P. lima increased over time; OA concentration and MRP activity conformably increased under N-and P-limited conditions compared with control counterparts. These changes in OA concentration and associated increased MRP activity suggest that MRP might be involved in OA transport in P. lima. However, MK571 and CsA, a broad-spectrum inhibitor of multidrug resistance (MDR)-associated ABC proteins, had no observable effects on OA content in P. lima. It seems that except for MDR-associated ABC proteins, other transporters like PDR might be involved in the transport of OA in P. lima.

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Plant Pleiotropic Drug Resistance Transporters: Transport Mechanism, Gene Expression, and Function

Cited by 60 publications

References 108 publications

The LABCG2 Transporter from the Protozoan Parasite Leishmania Is Involved in Antimony Resistance

The LABCG2 Transporter from the Protozoan Parasite Leishmania Is Involved in Antimony Resistance

Transcriptomic Analysis of Responses to Imbalanced Carbon: Nitrogen Availabilities in Rice Seedlings

MRP functional activity and character in the dinoflagellate Prorocentrum lima

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